Phenotypic and genotypic characterisation of Pasteurella multocida strains isolated from pigs in Hungary

Varga, Zsuzsanna; Sellyei, Boglárka; Magyar, Tibor

doi:10.1556/avet.55.2007.4.2

Cited by 16 publications

(15 citation statements)

References 24 publications

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“…On the other hand, although the determination of biovars is based on several (9-12) fermentation characters, the majority of the strains have been reported to belong only to a low number of biovars. Biovar 3 was shown to be the most common biovar, the presence of which seems largely independent of the host species (Fegan et al, 1995;Blackall et al, 1997Blackall et al, , 1998Townsend et al, 1998;Varga et al, 2007). In our study, biochemical fermentation patterns identified 7 different biovars (Table 2).…”

Section: Resultssupporting

confidence: 59%

Characterisation and comparison of avian Pasteurella multocida strains by conventional and ERIC-PCR assays

Sellyei

Varga

Ivánics

et al. 2008

Acta Veterinaria Hungarica

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Sixty-one avian strains of Pasteurella multocida were characterised and compared by biochemical tests, capsular PCR typing and ERIC-PCR. The strains were recovered from various avian species (goose, duck, Muscovy duck, turkey, chicken and pheasant) and represented different geographic locations in Hungary. Forty-two strains (69%) were identified as P. multocida subsp. multocida and 19 strains (31%) as P. multocida subsp. septica. The strains were grouped into 7 different biovars (1, 2, 3, 4, 5, 6 and 7). The most prevalent biovars were 1 (25%), 3 (21%) and 6 (21%). Most of the duck isolates (90%) belonged to biovar 1 or 6. The most frequent capsular type was A (93.5%). Type F represented only a small number (6.5%) of the strains. Other capsular types were not identified. From the 61 isolates 24 different fingerprint patterns were generated by ERIC-PCR assay. Based on cluster analysis the strains could be grouped into four larger and four miniclusters that showed considerable correlation with the geographical origin and the host species. The results indicate that ERIC-PCR may be a suitable technique for studying the host adaptation of P. multocida and the epidemiology of fowl cholera. Key words: Pasteurella multocida, poultry, capsular type, biovar, ERIC-PCRPasteurella multocida is a Gram-negative, non-motile, non-spore-forming, facultative anaerobic bacterium that can infect a wide range of wild and domesticated mammals and avian species, causing a variety of diseases. As a primary infectious agent it is responsible for fowl cholera in poultry (Rimler and Rhoades,

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Section: Resultssupporting

confidence: 59%

Characterisation and comparison of avian Pasteurella multocida strains by conventional and ERIC-PCR assays

Sellyei

Varga

Ivánics

et al. 2008

Acta Veterinaria Hungarica

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“…Sixteen PMT-negative and 8 PMT-positive strains were isolated from swine suffering from pneumonia. The strains had been characterised for other phenotypic and genotypic features earlier (Varga et al, 2007;Sellyei et al, 2008).…”

Section: Bacterial Strainsmentioning

confidence: 99%

Antimicrobial susceptibility of Pasteurella multocida isolated from swine and poultry

Sellyei

Varga

Szentesi-Samu

et al. 2009

Acta Veterinaria Hungarica

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Pasteurella multocida causes infectious diseases in a wide range of animal species. Antimicrobial therapy is still an effective tool for treatment. Generally, P. multocida isolates are susceptible to most of the widely used commercial antimicrobial agents but their excessive and unjustified use accelerates the emergence of resistant strains. We defined the antimicrobial sensitivity pattern of 56 P. multocida strains isolated from poultry (20) and swine [16 P. multocida toxin (PMT) positive and 20 PMT negative] to 16 widely applied antibiotics (apramycin, cefquinome, chloramphenicol, colistin, doxycycline, enrofloxacin, erythromycin, florfenicol, flumequine, neomycin, oxolinic acid, penicillin, trimethoprim potentiated sulphamethoxazole, sulphonamide compounds, tetracycline, tulathromycin) by the disk diffusion method. The majority of the strains was susceptible to most of the antimicrobial agents tested. However, the resistance to sulphonamides, tetracyclines, first-generation quinolones and aminoglycosides was remarkable, and thus the use of these compounds for the treatment of infection caused by P. multocida is not recommended. On the other hand, the antimicrobial activity of the classical penicillin, the newer macrolide (tulathromycin), the third-generation fluoroquinolone (enrofloxacin) and the fourth-generation cephalosporin (cefquinome) proved to be satisfactory against this bacterium.

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“…Bisgaard et al (3) determined the subspecies by their carbohydrate fermentation patterns. Based on the diversity to fermentation of different carbohydrates, Fegan et al (13) described 10 biovars among Pm strains, and later Blackall et al (4) added four other biovars to the classification system (37).…”

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confidence: 99%

Phenotypic and Genotypic Characterisation of Pasteurella Multocida Strains Isolated from Pigs in Poland

Stępniwska

Markowska-Daniel

2013

Bulletin of the Veterinary Institute in Pulawy

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A total of 319 Pasteurella multocida (Pm) strains isolated from pigs in Poland were examined. Phenotypic characterisation included: biochemical tests (to determine species, subspecies, and biovar), capsular typing, and antimicrobial susceptibility. Genotypic characterisation included detection of the toxA gene by PCR. All tested Pm strains were classified as Pm subsp. multocida: 87.2% biovar 3, 10.7%-2 and 0.9%-12. One strain was classified as biovar 1. Three strains of Pm did not suit any of the biovars. Using capsular typing methods, 77% of Pm strains isolated from nasal swabs belonged to type D and 33% to type A. Among Pm strains isolated from internal organs, 59.5% belonged to type A and 40.5% to type D. All the isolates showed a high susceptibility to β-lactams: ampicillin and amoxicilin with clavulonic acid (97.8%), penicillin (86.7%), doxicilline (100%), oxytetracycline (97.8%), and tetracycline (93.2%). It was found that all strains were susceptible to norfloxacin, 97.8% to enrofloxacin, and 95.6% to SxT. 24.4% and 15.6% of the strains were resistant to linco-spectin and tiamulin, respectively. The presence of toxA gene was confirmed by PCR in 20.8% of the strains isolated from nasal swabs and 29.1% of isolates from internal organs.

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Phenotypic and genotypic characterisation of Pasteurella multocida strains isolated from pigs in Hungary

Cited by 16 publications

References 24 publications

Characterisation and comparison of avian Pasteurella multocida strains by conventional and ERIC-PCR assays

Characterisation and comparison of avian Pasteurella multocida strains by conventional and ERIC-PCR assays

Antimicrobial susceptibility of Pasteurella multocida isolated from swine and poultry

Phenotypic and Genotypic Characterisation of Pasteurella Multocida Strains Isolated from Pigs in Poland

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