Phenotypic and Genotypic Characterisation of Pasteurella Multocida Strains Isolated from Pigs in Poland

Abstract

A total of 319 Pasteurella multocida (Pm) strains isolated from pigs in Poland were examined. Phenotypic characterisation included: biochemical tests (to determine species, subspecies, and biovar), capsular typing, and antimicrobial susceptibility. Genotypic characterisation included detection of the toxA gene by PCR. All tested Pm strains were classified as Pm subsp. multocida: 87.2% biovar 3, 10.7%-2 and 0.9%-12. One strain was classified as biovar 1. Three strains of Pm did not suit any of the biovars. Using capsular typing methods, 77% of Pm strains isolated from nasal swabs belonged to type D and 33% to type A. Among Pm strains isolated from internal organs, 59.5% belonged to type A and 40.5% to type D. All the isolates showed a high susceptibility to β-lactams: ampicillin and amoxicilin with clavulonic acid (97.8%), penicillin (86.7%), doxicilline (100%), oxytetracycline (97.8%), and tetracycline (93.2%). It was found that all strains were susceptible to norfloxacin, 97.8% to enrofloxacin, and 95.6% to SxT. 24.4% and 15.6% of the strains were resistant to linco-spectin and tiamulin, respectively. The presence of toxA gene was confirmed by PCR in 20.8% of the strains isolated from nasal swabs and 29.1% of isolates from internal organs.

The Prevalence of Pasteurella Multocida from Farm Pigs in Serbia

“…A lower percentage of 33% isolation of P. multocida from lung pigs was found in Serbia (11). The PCR method confirms that all 15 investigated strains of P. multocida belong to type A, which is in agreement with the reports of Stepniewska (12).…”

Virulence gene profiling of porcine Pasteurella multocida isolates of Assam

“…During the present study, some of the P. multocida isolates could not produce mortality in mice following inoculation with P. multocida isolate having virulence gene, either alone or in combination. This might be due to repeated subculturing of the isolates in laboratory media that result in the suppression of gene function or due to gene mutation, resulting in non-expression of the genes in vivo [ 31 , 32 ]. Detection of a high proportion of toxigenic capsular type A P. multocida from pigs was also reported by other workers [ 11 , 20 , 30 , 33 ].…”

Development of Experimental Oil based Inactivated HS Vaccine from field Isolates of Pasteurella multocida from Cattle in Bangladesh

“…Hemorrhagic septicemia is an economically important bacterial disease of cattle and buffaloes. 6 The disease remains a significant obstacle to sustainable livestock production in most parts of tropical Asia and Africa. It is caused by Pasteurella multocidaa natural inhabitant of the mucosal surfaces of upper part of the respiratory tract of ruminants, and under predisposing environmental or management conditions which constitute stress for the animals such as transport (shipping fever), marketing, change of feed, climate or ventilation.…”

“…The oil based vaccine which persisted for more than six months due to viscosity of the adjuvants was low (easily injectable) and the oil phase did not separate upon centrifugation at 1,000g for 30minutes or standing at room temperature for 30days. [5][6][7] Keeping in view the above facts, the present study has been undertaken to developed oil based inactivated HS vaccine from the field isolates ofPasteurella multocida in cattle in Bangladesh and determination of PHA titres of the experimentally prepared oil based inactivated HS vaccine in bovines.…”