Characterisation and comparison of avian Pasteurella multocida strains by conventional and ERIC-PCR assays

Abstract

Sixty-one avian strains of Pasteurella multocida were characterised and compared by biochemical tests, capsular PCR typing and ERIC-PCR. The strains were recovered from various avian species (goose, duck, Muscovy duck, turkey, chicken and pheasant) and represented different geographic locations in Hungary. Forty-two strains (69%) were identified as P. multocida subsp. multocida and 19 strains (31%) as P. multocida subsp. septica. The strains were grouped into 7 different biovars (1, 2, 3, 4, 5, 6 and 7). The most prevalent biovars were 1 (25%), 3 (21%) and 6 (21%). Most of the duck isolates (90%) belonged to biovar 1 or 6. The most frequent capsular type was A (93.5%). Type F represented only a small number (6.5%) of the strains. Other capsular types were not identified. From the 61 isolates 24 different fingerprint patterns were generated by ERIC-PCR assay. Based on cluster analysis the strains could be grouped into four larger and four miniclusters that showed considerable correlation with the geographical origin and the host species. The results indicate that ERIC-PCR may be a suitable technique for studying the host adaptation of P. multocida and the epidemiology of fowl cholera. Key words: Pasteurella multocida, poultry, capsular type, biovar, ERIC-PCRPasteurella multocida is a Gram-negative, non-motile, non-spore-forming, facultative anaerobic bacterium that can infect a wide range of wild and domesticated mammals and avian species, causing a variety of diseases. As a primary infectious agent it is responsible for fowl cholera in poultry (Rimler and Rhoades,

Comparison and Utilization of Repetitive-Element PCR Techniques for Typing Lactobacillus Isolates from the Chicken Gastrointestinal Tract

“…The current research extends the application of ERIC-PCR, which has previously been used for typing a wide variety of gram-negative bacteria in chickens, including Arcobacter spp. (5), Escherichia coli (17), Salmonella enterica (13), Campylobacter jejuni (70), Pasteurella multocida (52), and Ornithobacterium rhinotracheale (60). Collectively, the current research and these past studies highlight the utility of ERIC-PCR in the typing of a variety of Isolates of enterococci were also able to be typed to the species and ERIC type levels, supporting a previous assessment of the ERIC-PCR for the differentiation of E. faecium isolates (18).…”

Antimicrobial susceptibility of Pasteurella multocida isolated from swine and poultry

“…Sixteen PMT-negative and 8 PMT-positive strains were isolated from swine suffering from pneumonia. The strains had been characterised for other phenotypic and genotypic features earlier (Varga et al, 2007;Sellyei et al, 2008).…”

Sequencing-independent method for the differentiation of the main phylogenetic lineages ofPasteurella multocida

“…11,13 The presumption is that P. multocida subsp. gallicida strains are dulcitol and sorbitol positive but trehalose negative; subsp.…”