Phenotype, genotype, and serotype distribution of macrolide resistant invasive and non-invasive Streptococcus pneumoniae strains, in Sousse, Tunisia

Marzouk, Manel; Ferjani, Asma; Amamou, S.; Alibí, Sana; Ali, Mahboob; Boukadida, Jalel

doi:10.1016/j.medmal.2014.07.016

Cited by 6 publications

(6 citation statements)

References 20 publications

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“…Similar to the other reports from Middle East countries [3, 25] the frequencies of our serotypes included in PCV7 and PCV10 were 89% for clinical, 50% for normal flora and 68% for total sisolates. Serotype 3 was the only non-PCV7 serotype found in our clinical study.…”

Section: Discussionsupporting

confidence: 90%

“…Similar to the results obtained in Asia and some European countries [3, 14–16], we also found that the most common mechanism (48%) for macrolide resistance in our pneumococcal isolates was ermB-encoded ribosome methylase activity. On the contrary, efflux mediated pump gene ( mef ), which has been reported to be the dominant macrolide resistant in North American countries [17–18] and Germany [19], was found only in 20% of our isolates.…”

Section: Discussionsupporting

confidence: 90%

“…Macrolides are the most frequently prescribed antibiotic among CA pneumococcal infections [3]. Macrolide-resistance (MR) in pneumococci is mediated by two major mechanisms including target site modification by methylases, encoded by ermB (MLS B phenotype) and efflux pump mechanism mediated by mef (M phenotype) genes with various transposons [4–6].…”

Section: Introducationmentioning

confidence: 99%

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Determination of Characteristics of Erythromycin Resistant Streptococcus pneumoniae with Preferred PCV Usage in Iran

et al. 2016

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Amongst 100 Streptococcus pneumoniae isolated from clinical cases and nasopharynx of healthy individuals, 60 erythromycin resistant strains were isolated and characterized using MLST, PFGE, transposon analysis and Quellung reaction. Most of the S. pneumoniae erythromycin resistant (80%) were found to be attributable to the ermB-edncoded ribosome methylase activity which differs from the dominant mechanism of macrolide resistance seen in North America.The most predominant transposons were; Tn1545/6003 (27%), Tn6002 (22%), Tn2009 (20%), Tn2010 (17%). Number of the clinical isolates carrying Tn2010 was more significant than the normal flora. The serotypes found were; 14 (33%), 3 (22%), 23F (15%), 19F (15%), 19A (7%), 6A (3%), 9V (3%) and 6B (2%). The most prevalent serotypes among the clinical (n = 28) and normal flora (n = 32) isolates were serotypes 14 (46%) and 3 (31%), respectively. The most prevalent vaccine serotypes amongst the clinical isolates and the healthy individuals were pneumococcal conjugate vaccines (PCV) 13 and PCV10, respectively. PFGE revealed 34 pulsotypes with 9 common and 25 single types. Significant number of the normal isolates belonged to CT5 and CT6. On the other hand, significant number of clinical isolates belonged to CT8 as compared to the normal flora isolates. MLST showed 2 dominant sequence types. ST3130 (23%) and ST180 (22%) were the most predominant sequence types in the clinical and normal isolates, respectively. There was no significant difference in other sequence types between clinical and normal flora isolates. Three polyclonal complexes including Sweden15A -25, Spain23F-1 and Spain9V-3 constituted 58% of the isolates. Our results suggest that the genetic diversity and transposon distribution were high among S. pneumoniae, particularly in the isolates containing erm(B) and double antibiotic resistant genes (erm/mef). The results presented here could influence the change in the current vaccination practices in Iran which currently calls for vaccination with PCV7 or PCV10.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 90%

Section: Introducationmentioning

confidence: 99%

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Determination of Characteristics of Erythromycin Resistant Streptococcus pneumoniae with Preferred PCV Usage in Iran

et al. 2016

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“…Of interest, the presence of serotype 3 (9.2%) was noted in this study. This serotype, described in several countries, has presented a low prevalence in reports from Tunisia and North Africa (Ziane et al, 2016;Charfi et al, 2012;Rachdi et al, 2011;Marzouk et al, 2014Marzouk et al, , 2015Raddaoui et al, 2015;Diawara et al, 2015). As has been reported previously (Martens et al, 2004), serotype 3 was most often isolated from non-invasive diseases in the present study (82.1%).…”

Section: Discussionsupporting

confidence: 81%

“…The choice of vaccine in each country must take into account the circulating serotypes. Current local data on pneumococcal serotypes in Tunisia are limited, and only a few reports from Tunisia have been published in the last decade (Charfi et al, 2012;Rachdi et al, 2011;Marzouk et al, 2014Marzouk et al, , 2015Raddaoui et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Serotype distribution and antibiotic susceptibility of Streptococcus pneumoniae strains in the south of Tunisia: A five-year study (2012–2016) of pediatric and adult populations

Ktari

Jmal

Mroua

et al. 2017

International Journal of Infectious Diseases

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High prevalence of multidrug-resistant international clones among macrolide-resistant Streptococcus pneumoniae isolates in immunocompromised patients in Tunisia

Raddaoui

Tanfous

Chebbi

et al. 2018

International Journal of Antimicrobial Agents

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Phenotype, genotype, and serotype distribution of macrolide resistant invasive and non-invasive Streptococcus pneumoniae strains, in Sousse, Tunisia

Cited by 6 publications

References 20 publications

Determination of Characteristics of Erythromycin Resistant Streptococcus pneumoniae with Preferred PCV Usage in Iran

Determination of Characteristics of Erythromycin Resistant Streptococcus pneumoniae with Preferred PCV Usage in Iran

Serotype distribution and antibiotic susceptibility of Streptococcus pneumoniae strains in the south of Tunisia: A five-year study (2012–2016) of pediatric and adult populations

High prevalence of multidrug-resistant international clones among macrolide-resistant Streptococcus pneumoniae isolates in immunocompromised patients in Tunisia

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