Serotype distribution and antibiotic susceptibility of Streptococcus pneumoniae strains in the south of Tunisia: A five-year study (2012–2016) of pediatric and adult populations

Ktari, Sonia; Jmal, Ikram; Mroua, Manel; Maâlej, Sami; Ayed, Nour El Houda Ben; Mnif, Basma; Rhimi, Faouzia; Hammami, Adnène

doi:10.1016/j.ijid.2017.10.015

Cited by 22 publications

(12 citation statements)

References 28 publications

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“…Besides erythromycin resistance, tetracycline and co-trimoxazole resistance was found to be 42.9% and 35.9%, respectively. Similar resistance rates have also been reported in a related study among children less than 5 years of age (Ktari et al, 2017;Ganaie et al, 2016). MDR S. pneumoniae (MDRSP) is also being reported progressively in Malaysia and other Asian countries (Ganaie et al, 2016;Song et al, 2004).…”

Section: Antibioticsupporting

confidence: 87%

“…In this study, a high rate of resistance to antibiotics other than penicillin was found. The erythromycin resistance rate at 42.9% is still comparatively lower than the rates reported in studies performed in India (71.4%), Tunisia (64.7%), and Korea (73.3%) (Ktari et al, 2017;Ganaie et al, 2016;Song et al, 2004). Besides erythromycin resistance, tetracycline and co-trimoxazole resistance was found to be 42.9% and 35.9%, respectively.…”

Section: Antibioticmentioning

confidence: 76%

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Pneumococcal serotype distribution and antibiotic susceptibility in Malaysia: A four-year study (2014–2017) on invasive paediatric isolates

Arushothy

Ahmad

Amran

et al. 2019

International Journal of Infectious Diseases

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This study was performed to analyze the serotype distribution of Streptococcus pneumoniae causing invasive pneumococcal disease (IPD) in children aged 5 years and under in Malaysia and to assess the antimicrobial resistance. Methods: From 2014 to 2017, a total of 245 invasive S. pneumoniae isolates from children 5 years of age were received from hospitals all around Malaysia. All isolates were identified and subjected to serotyping and antimicrobial susceptibility testing. Results: Of the 245 isolates, 117 (48.0%) were from children aged <1 year, 46 (19.05%) were from children aged 1-2 years, and 82 (33.0%) were from children aged 2-5 years. The most common serotypes were 14 (26.9%), 6B (19.6%), 19A (11.8%), 6A (10.6%), and 19F (6.9%) and vaccine coverage was 88.2% for PCV13, 64.1% for PCV10, and 63.3% for PCV7. Resistance to penicillin was 0.2% for non-meningitis cases and 22.2% for meningitis cases; erythromycin resistance was reported in 42.9%, co-trimoxazole in 35.9%, and tetracycline in 42.9%. Conclusions: Serotypes 14, 6B, 19A, 6A, and 19F were the most common serotypes isolated from children with IPD in Malaysia during this pre-vaccination era. The lack of reports on the serotype distribution has limited action for the implementation of PCV in the national immunization programme (NIP). The information from this study may benefit future policies for the introduction of PCV in the Malaysian NIP and ultimately may reduce the morbidity and mortality among children in Malaysia.

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Section: Antibioticsupporting

confidence: 87%

Section: Antibioticmentioning

confidence: 76%

Pneumococcal serotype distribution and antibiotic susceptibility in Malaysia: A four-year study (2014–2017) on invasive paediatric isolates

Arushothy

Ahmad

Amran

et al. 2019

International Journal of Infectious Diseases

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“…Pneumococcal strains were non invasive and belonged to a variety of capsular types (14, 19A, 3 and 9V/A). These serotypes were commonly found in our region, South of Tunisia [28]. It has been reported that serotype distribution among Opt r S. pneumoniae strains is mostly dependent on the epidemiological profile of each area [5][6][7].…”

Section: Discussionmentioning

confidence: 66%

Clinical optochin resistant Streptococcus pneumoniae and Streptococcus pseudopneumoniae strains in Tunisia

Ktari

Ayed

Maâlej

et al. 2021

J Infect Dev Ctries

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Introduction: Streptococcus pneumoniae can be responsible for severe human infections. Optochin resistance has been a potential cause of misidentification of pneumococcus and other members of the mitis group. Hence, rapid and easy optochin resistant (Optr) S. pneumoniae identification is essential. Methodology: Atypical pneumococci were characterized using optochin susceptibility, bile solubility based on spectrophotometric reading, serotyping, pulsed field gel electrophoresis (PFGE), 16S rRNA sequencing and PCR-based assays targeting pneumococcal genes lytA, ply, pspA, cpsA, Spn9802 and Spn9828. Results: Optical density values for the bile solubility test suggest the identification of four Optr S. pneumoniae and one Streptococcus pseudopneumoniae. All Optr pneumococci harbored cpsA, lytA, ply, Spn9802, Spn9828 and pspA genes. Only ply, spn9802 and Spn9828 genes were detected in S. pseudopneumoniae. The 16S rRNA sequencing differentiates between these two species. Optr S. pneumoniae strains belonged to different genotypes and serotypes (14, 19A, 3 and 9V). Three Optr S. pneumoniae isolates were typed as pspA family 2, while one belonged to pspA family 1. Sequencing of the atpA and atpC gene of the Optr variants revealed three mutations in the ATPase a-subunit (L99I, M23V and V52I) and one mutation in ATPase c-subunit (V48I). Conclusions: Our data indicate that bile OD-values provides an accurate, fast and easy method to discriminate between Optr S. pneumoniae and other Streptococcus mitis group. Moreover molecular techniques, confirming the bile test, can be used in order to prevent these atypical pneumococci and alert clinical microbiologists of the presence of these strains in the community.

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“…1; Table 3). Across these publications, data were provided for Algeria (n = 1) [43], Egypt (n = 5) [43][44][45][46][47], Iran (n = 2) [48,49], Kuwait (n = 3) [50][51][52], Lebanon (n = 3) [53][54][55], Morocco (n = 2) [43,56], Oman (n = 1) [57], Saudi Arabia (n = 2) [51,58], Tunisia (n = 6) [43,[59][60][61][62][63], Turkey (n = 5) [64][65][66][67][68], and Yemen (n = 1) [40].…”

Section: Streptococcus Pneumoniaementioning

confidence: 99%

The Burden of Invasive Vaccine-Preventable Diseases in Adults in the Middle East and North Africa (MENA) Region

et al. 2021

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Serotype distribution and antibiotic susceptibility of Streptococcus pneumoniae strains in the south of Tunisia: A five-year study (2012–2016) of pediatric and adult populations

Cited by 22 publications

References 28 publications

Pneumococcal serotype distribution and antibiotic susceptibility in Malaysia: A four-year study (2014–2017) on invasive paediatric isolates

Pneumococcal serotype distribution and antibiotic susceptibility in Malaysia: A four-year study (2014–2017) on invasive paediatric isolates

Clinical optochin resistant Streptococcus pneumoniae and Streptococcus pseudopneumoniae strains in Tunisia

The Burden of Invasive Vaccine-Preventable Diseases in Adults in the Middle East and North Africa (MENA) Region

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