High prevalence of multidrug-resistant international clones among macrolide-resistant Streptococcus pneumoniae isolates in immunocompromised patients in Tunisia

Raddaoui, Anis; Tanfous, Farah Ben; Chebbi, Yosra; Achour, Wafa; Baaboura, Rekaya; Benhassen, Assia

doi:10.1016/j.ijantimicag.2018.04.015

Cited by 18 publications

(18 citation statements)

References 23 publications

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“…The incidence of different ESBLs is described by Kharrat et al following epidemiological and molecular characterisation of Enterobacteriaceae-infected haematopoietic stem cell transplant recipients hospitalised at the Tunisian National Bone Marrow Transplant Center [3] . Another study by Raddaoui et al on Gram-positive bacteria reports a high prevalence of multidrug-resistant international clones among macrolide-resistant Streptococcus pneumoniae strains isolated in immunocompromised patients in Tunisia [4] . In addition to β-lactamase production, porin alteration is a significant resistance mechanism.…”

Section: Recent Advances In Antimicrobial and Bacterial Resistancementioning

confidence: 97%

Recent advances in antimicrobial and bacterial resistance

Chouchani¹,

Rolain

Ghrairi

2018

International Journal of Antimicrobial Agents

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HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

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Section: Recent Advances In Antimicrobial and Bacterial Resistancementioning

confidence: 97%

Recent advances in antimicrobial and bacterial resistance

Chouchani¹,

Rolain

Ghrairi

2018

International Journal of Antimicrobial Agents

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“…Generally, β-lactams are the preferred drugs for the treatment of Streptococcus agalactiae infection, but for allergic patients, macrolides and related drugs are effective alternatives. At present, the mainly known mechanism of macrolide antibiotics inhibiting Streptococcus is the target modi cation of 23s rRNA methylase encoded by [7] ribosome methylation erm gene [8]. The methylation of rRNA can lead to the change of ribosome con guration, resulting in the decrease in the binding ability of macrolides, glycosamides, and streptogramins B to bacterial ribosome targets [7].…”

Section: Introductionmentioning

confidence: 99%

“…The methylation of rRNA can lead to the change of ribosome con guration, resulting in the decrease in the binding ability of macrolides, glycosamides, and streptogramins B to bacterial ribosome targets [7]. Since these antibiotics have the same or overlapping target sites, bacteria can simultaneously cross-resistant to these three types of antibiotics, known as macrolide-lincosamide-streptogramin B (MLS B ) resistance [8,9]. The common drug-resistance related genes in Streptococcus are ermA, ermB, and ermC [10].…”

Section: Introductionmentioning

confidence: 99%

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Infection and drug resistance of Streptococcus agalactiae among perinatal pregnant women in Xinjiang

Guo

Luo

Jia

et al. 2021

Preprint

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Background: Group B streptococcus (Streptococcus agalactiae) is one of the most common pathogens causing meningitis, bacteremia and pneumonia. The drug resistance mechanisms of group B streptococcus in different countries and regions also show regional differences.Method: The study population was comprised of 1877 pregnant women of 34-38 weeks who underwent prenatal examination in the gynecology and obstetrics outpatient clinic of Xinjiang People's Hospital, between January 1, 2019 and January 31, 2020. Clinic specimens were collected and identified by the API bacteria Rapid Identification card for the downstream group B Streptococcus (Streptococcus agalactiae) isolation. Drug susceptibility of the Streptococcus agalactiae isolated was detected by Kirby-Bauer disk diffusion method. Macrolide–lincosamide–streptogramin B (MLSB) resistance was determined by D test. Real-time quantitative polymerase chain reaction and Gene sequencing was performed for the resistance genes ermA, ermB, mreA, erm (47), mefA/E and Lin B.Results: 149 Streptococcus agalactiae-positive strains were identified by clinical isolation, with a positive rate of 7.94%. Group B Streptococcus showed 100% susceptibility to linezolid, penicillin, vancomycin, meropenem, ampicillin, ceftriaxone, 44.97%, 35.57%, 56.38% and 29.53% susceptibility to levofloxacin, erythromycin, tetracycline and clindamycin, respectively. Among the 149 isolates, 127 strains showed macrolide resistance phenotype. The detection rate of intrinsic resistance phenotype (cMLS) was 40.94% (59/127), active efflux resistance phenotype (MS) 9.45% (12/127), and induced resistance phenotype (iMLS) 22.83% (29/127).Conclusion: The ermB gene-mediated 50s ribosome target site change co-existing with mreA gene for macrolide resistance efflux may play a major role in the mechanism of Streptococcus agalactiae resistance macrolide resistance of in perinatal women in Xinjiang. The change of 50s ribosomal target site mediated by ermB gene may be the main reason for drug cross-resistance.

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“…The problem of antibiotic abuse has become prominent, especially in China, and emerging pathogenic bacteria have developed resistance to single and even multiple antibiotics (6)(7)(8)(9). Moreover, super bacteria, including methicillinresistant Staphylococcus aureus (MRSA) (10,11), multidrugresistant Streptococcus pneumoniae (MDRSP) (12), and multidrug resistance Acinetobacter baumannii (MRAB) (13) have recently emerged and rapidly developed a high prevalence. According to drug resistance monitoring data in China, up to 33% of isolated strains in 3-A grade hospitals are multi-drug resistant (14).…”

Section: Introductionmentioning

confidence: 99%

Application of next generation sequencing-based rapid detection platform for microbiological diagnosis and drug resistance prediction in acute lower respiratory infection

Chao¹,

Li²,

Zhang³

et al. 2020

Ann Transl Med

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Background: Acute lower respiratory infections (ALRIs) have a high mortality rate. We aimed to apply a platform that rapidly detects 36 microorganisms and 49 antibiotic resistance markers in the clinical diagnosis of ALRI and drug resistance prediction.Methods: Multicenter collection of clinical samples from patients with ALRIs was carried out from 2017 to 2018. Sputum culture (SC) was performed, which provided two outcomes: the detected pathogens and the resistance to different antibiotics. Additionally, each sputum sample was used to extract deoxyribonucleic acids (DNAs) followed by high-throughput sequencing.Results: Eleven commonly observed pathogens were surveyed, and for all samples with positive SC results (137 cases), the overall coverage was 95.62% according to the sequencing results. The receiver operating characteristic (ROC) curve was drawn, and cutoff reads of the most frequently detected pathogens were acquired. Overall, sequencing exhibited significantly higher sensitivity in the detection of pathogens compared with the traditional SC method, with a generally satisfactory specificity. Furthermore, we investigated the correlation between antibiotic resistance gene phenotypes and the actual outcomes of the drug sensitivity test, and some significant correlations were found, especially for the resistance to Amikacin in the presence of blaOXA7.Conclusions: Sequencing-based sputum metagenomics can reveal a profile of the lung pathogen microbiome. The sequencing method offers both sufficient accuracy and significantly higher sensitivity in the detection of pathogens, and can be at least a complementary approach to traditional SC reporting. The sequencing technique also revealed some novel potential correlations between the presence of different pathogens, as well as new antimicrobial-resistant genes.

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High prevalence of multidrug-resistant international clones among macrolide-resistant Streptococcus pneumoniae isolates in immunocompromised patients in Tunisia

Cited by 18 publications

References 23 publications

Recent advances in antimicrobial and bacterial resistance

Recent advances in antimicrobial and bacterial resistance

Infection and drug resistance of Streptococcus agalactiae among perinatal pregnant women in Xinjiang

Application of next generation sequencing-based rapid detection platform for microbiological diagnosis and drug resistance prediction in acute lower respiratory infection

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