1966
DOI: 10.1093/clinchem/12.8.482
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Phenolphthalein Monophosphate as a Substrate for Serum Alkaline Phosphatase

Abstract: A rapid and precise procedure for serum alkaline phosphatase employs phenolphthalein monophosphate as the substrate. This compound is readily hydrolyzed and directly provides a chromogen for colorimetric determination. The color produced is linear with respect to both enzyme concentration and incubation time and is stable. Reagent and serum blanks are negligible. Various factors which affect alkaline phosphatase activity have been evaluated. The importance of the proper selection of buffer species, concentrati… Show more

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Cited by 205 publications
(63 citation statements)
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“…Concentrations of the enzyme alkaline phosphatase were determined in serum in trials 111 to VIII and in urine in trials VII and VIII, according to the method of BABSON et al [3]. Lacticdehydrogenase (LDH) concentrations in serum in trials V to VII and urine in trial VII were determined according to the method of BERGER and BROIDA [7].…”
Section: Methodsmentioning
confidence: 99%
“…Concentrations of the enzyme alkaline phosphatase were determined in serum in trials 111 to VIII and in urine in trials VII and VIII, according to the method of BABSON et al [3]. Lacticdehydrogenase (LDH) concentrations in serum in trials V to VII and urine in trial VII were determined according to the method of BERGER and BROIDA [7].…”
Section: Methodsmentioning
confidence: 99%
“…Ca, and Kraml (1966) for P and by a modification of the Babson (1965) phenolphthalein monophosphate method for alkaline phosphatase. The Ca, P and Zn concentrations in liver and bone ash and Zn concentration in plasma were determined by atomic absorption spectrophotometry.…”
Section: Methodsmentioning
confidence: 99%
“…The aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities were determined according to Reitman and Frankel (1957) colometric method using Randox kits, while alkaline phosphatase activity was determined according to phenolphthalein monophosphate method (Babson et al, 1966).…”
Section: Serum Enzymesmentioning
confidence: 99%