Phenolphthalein Monophosphate as a Substrate for Serum Alkaline Phosphatase

Babson, Arthur L.; Greeley, Sharon; Coleman, Charles M.; Phillips, George E.

doi:10.1093/clinchem/12.8.482

Cited by 205 publications

(63 citation statements)

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“…Concentrations of the enzyme alkaline phosphatase were determined in serum in trials 111 to VIII and in urine in trials VII and VIII, according to the method of BABSON et al [3]. Lacticdehydrogenase (LDH) concentrations in serum in trials V to VII and urine in trial VII were determined according to the method of BERGER and BROIDA [7].…”

Section: Methodsmentioning

confidence: 99%

Ochratoxicosis in Beagle Dogs. 1. Clinical and Clinicopathological Features

1973

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Abstunct. Rice culture of Aspergillus ochraceus that contained ochratoxin A or pure crystalline ochratoxin A was administered orally daily to 23 young male Beagle dogs. Six other young male Beagles served as controls. Most dogs were killed in extuenzis on or before day 14 after receiving a daily oral dose of ochratoxin A, 0.2-3.0 nig/kg body weight. Consistent clinical features of the mycotoxicosis were anorexia, weight loss, emesis, tenesmus, passage of clots of blood-stained mucus from the rectum, rectal temperature up to 107 "F, tonsillitis, dehydration and prostration. The prominent clinicopathological features of the toxicosis were a reflection of renal damage. The urine had a specific gravity as low as 1.004 and contained granular casts, necrotic renal epithelium, and concentrations above normal of protein, glucose, lactic dehydrogenase, isocitric dehydrogenase, leucine aminopeptidase, glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase, and alkaline phosphatase. Serum concentrations of these enzymes did not change. Concentrations of blood urea nitrogen remained, with two exceptions, within the normal range, but theconcentrations of blood glucose declined terminally. Serum concentrations of the liver-specific enzyme, y-glutamyl transpeptidase and sulfabromophthalein dye retention time did not indicate hepatic dysfunction.

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Section: Methodsmentioning

confidence: 99%

Ochratoxicosis in Beagle Dogs. 1. Clinical and Clinicopathological Features

1973

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“…Ca, and Kraml (1966) for P and by a modification of the Babson (1965) phenolphthalein monophosphate method for alkaline phosphatase. The Ca, P and Zn concentrations in liver and bone ash and Zn concentration in plasma were determined by atomic absorption spectrophotometry.…”

Section: Methodsmentioning

confidence: 99%

Effect of Dietary Calcium and Zinc Levels on Weight Gain and Blood and Tissue Mineral Concentrations of Growing Columbia- and Suffolk-Sired Lambs

Wg¹

1983

Journal of Animal Science

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One hundred and sixty Columbia- and Suffolk-cross intact male and female growing lambs (8 wk of age) were used in an 84-d feeding trial to determine the effect of two levels dietary Ca and Zn on weight gain, feed utilization and several blood and tissue traits. Two levels of Ca (.5 and .8%) and two levels of Zn (20 and 100 ppm) were fed in a 2 X 2 factorial arrangement of dietary treatments. There was no effect of diet on performance or on blood traits. Columbia lambs had heavier fleeces at slaughter than Suffolks (P less than .01) and males had heavier fleeces than females (P less than .01). Based on the similarity in performance among lambs fed the four diets differing in Ca and Zn level, it is concluded that a dietary Ca level of .5% and a Zn level of 19 to 26 ppm is adequate for normal weight gain, feed utilization, liver and humerus ash concentrations and blood plasma concentrations of Ca, P, Zn, total protein, albumin, alkaline phosphatase and blood hemoglobin and hematocrit. Further, it appears that an elevated level of dietary Ca does not precipitate a clinical Zn deficiency in the growing lamb, in contrast to the general observation in growing swine.

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“…The aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities were determined according to Reitman and Frankel (1957) colometric method using Randox kits, while alkaline phosphatase activity was determined according to phenolphthalein monophosphate method (Babson et al, 1966).…”

Section: Serum Enzymesmentioning

confidence: 99%

Growth, nutrient utilization, haematology and biochemical parameters of African catfish (<i>Clarias gariepinus</i>, Burchell, 1822) fed with varying levels of <i>Bacillus subtilis</i>

Lawal¹,

Lawal²,

Adewumi³

et al. 2019

Agrosearch

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This study examined the growth response, nutrient utilization, biochemical and haematological properties of Clarias gariepinus juveniles, fed with graded levels of Bacillus subtilis. Five diets were formulated (35% crude protein; 3127 kCal/kg energy), comprising 0 (T1), 20 mg/100g oxytetracycline (T2), 10 5 (T3), 10 7 (T4) and 10 9 (T5) B. subtilis CFU/ml. African catfish, C. gariepinus (n=150; mean weight =94.33±0.67g) were allotted to 15 rectangular tanks and fed experimental diets apparently to satiation for 8 weeks. Growth performance, nutrient utilization, haematological and biochemical parameters were examined using standard methods. The results showed that fish fed with Diet T5 recorded significantly high values for mean weight gain (MWG) (116.67±5.70g), specific growth rate (SGR) (1.58±0.07%) and percentage weight gain (PWG) (133.62±7.47%), while Diet T1 had least values for MWG (89.00±0.58g), SGR (1.36±0.01%) and PWG (94.35±0.91g). Feed conversion ratio (FCR) and protein efficiency ratio (PER) were significantly different (p<0.05) across the test diets, with Diet T5 having the best values for FCR (1.17±0.04) and PER (3.27±0.18). No significant differences (p>0.05) were observed in the haematological, AST, ALP and GSH indices between the fish fed graded levels of probiotic and control diets. The excellent growth performance recorded at the highest inclusion level (T5) of B. subtilis showed that the probiotic could be favourably incorporated into the diet of C. gariepinus juveniles.

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Phenolphthalein Monophosphate as a Substrate for Serum Alkaline Phosphatase

Cited by 205 publications

References 0 publications

Ochratoxicosis in Beagle Dogs. 1. Clinical and Clinicopathological Features

Ochratoxicosis in Beagle Dogs. 1. Clinical and Clinicopathological Features

Effect of Dietary Calcium and Zinc Levels on Weight Gain and Blood and Tissue Mineral Concentrations of Growing Columbia- and Suffolk-Sired Lambs

Growth, nutrient utilization, haematology and biochemical parameters of African catfish (<i>Clarias gariepinus</i>, Burchell, 1822) fed with varying levels of <i>Bacillus subtilis</i>

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