Phenolic Composition and Antioxidant Activity in Seed Coats of 60 Chinese Black Soybean (Glycine max L. Merr.) Varieties

Zhang, Rui Fen; Zhang, Fang Xuan; Zhang, Ming Wei; Wei, Zhen; Yang, Chun Ying; Zhang, Yan; Tang, Xiao; Deng, Yanrui; Wei, Jian He

doi:10.1021/jf201593n

Cited by 135 publications

(107 citation statements)

References 31 publications

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“…Anthocyanins are the second most important phytochemicals in black soybean besides isoflavones (Zhang et al 2011). As revealed in previous studies, anthocyanin contents in black soybeans ranged from ＜1.0 to 20.4 mg/g (Xu and Chang 2008a;2008b).…”

Section: Discussionmentioning

confidence: 66%

Anthocyanin and Isoflavone Contents in Korean Black Soybean Landraces and Their Antioxidant Activities

Lee

et al. 2016

Plant Breed. Biotech.

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Anthocyanin and isoflavone contents and antioxidant activities of 56 Korean black soybean landraces were examined in this study. Total isoflavone content of 56 Korean black soybean landraces ranged from 43.8 to 347.5 mg/100 g. Total anthocyanin content ranged from 19.8 to 1,420.4 mg/100 g, with cyaniding-3-glucoside being the most abundant anthocyanin in all landraces, ranging from 12.2 to 1,207.3 mg/100 g. Antioxidant activities based on 2,2-diphenyl-1-picrylhydrazyl, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid), ferric-reducing antioxidant power, and total polyphenol content assays showed wide variations, ranging from 17.1 to 76.6 (IC50), 53.5 to 127.7 (IC50), 5.4 to 34.2 mg ascorbic acid/g, and 9.7 to 36.8 mg gallic acid equivalents/g), respectively. Using relative antioxidant capacity index, IT156132 sample had the highest antioxidant activity. In clustering analysis, the 56 Korean black soybean landraces were classified into five groups. Group I consisted of four landraces with high isoflavone contents and antioxidant activities.

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Section: Discussionmentioning

confidence: 66%

Anthocyanin and Isoflavone Contents in Korean Black Soybean Landraces and Their Antioxidant Activities

Lee

et al. 2016

Plant Breed. Biotech.

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“…However, although research has demonstrated the antioxidant properties of soybean with black coat (ZHANG et al, 2011), it is important to note that the concentration of phenolic compounds varies from genotype to genotype and depends on tissue development stage and may also change, depending on the environment (LEPINIEC et al, 2006). The results obtained in this study do not fully agree with Lepiniec et al (2006), since there was no difference in the content of phenolic compounds as well as in the antioxidant potential between the genotypes with black seed coats, IAC and TP (Table 2).…”

Section: Resultsmentioning

confidence: 99%

Chemical composition and structural characterization of contrasting colors of soybean seed coats

et al. 2015

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Seed coats provide protection to seeds and present differential traits between soybean genotypes. The aim of this study was to determine the chemical composition and analyze the internal coat structure of soybean genotypes with contrasting seed coat color, as well as to evaluate the rate of water absorption by seeds. Four genotypes were tested: two genotypes with black seed coat (IAC and TP lines) and two genotypes with yellow seed coat (BMX Potência RR and CD 202 cultivars).The seeds of the four genotypes were grown during the 2012/0213 crop season, in a greenhouse at the Embrapa Clima Temperado -Estação Experimental Terras Baixas. After the harvest, the seeds were stored in controlled conditions for five months. After this period, concentration of phenolic compounds, antioxidant potential, anthocyanins, carotenoids, and lignin in seed coats were determined at the Universidade Federal de Pelotas. Internal coat structure was examined on anatomical slides and documented with image capturing software using a microscope at the Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo. Finally, water absorption of contrasting seeds was evaluated at 42 hours of imbibition. Experimental design was completely randomized with five replicates. Data were subjected to analysis of variance and the means were compared by Tukey's test, at 5% probability level. Coat structure was characterized by the image analysis. Imbibition data were subjected to regression analysis. The concentration of phenolic compounds, antioxidant potential, anthocyanins, carotenoids, and lignin are higher in black coated genotypes. Their palisade layer and hourglass cells are thicker and the former are more lignified in black seed coats compared to yellow seed coats. The water gain by the seeds is dependent on the conditions in which the seeds were formed and varied between genotypes, independent of coat coloration and lignin concentration. Key words: Anthocyanins, internal structure, lignin, water absorption ResumoOs tegumentos desempenham importante função de proteção às sementes, e podem apresentar características diferenciais entre os genótipos de soja. O objetivo do trabalho foi determinar a composição química e analisar a estrutura interna dos tegumentos de genótipos de soja com cores contrastantes, assim como avaliar a absorção de água destas sementes. Foram testados quatro genótipos sendo dois de tegumento preto (linhagens IAC e TP) e dois de tegumento amarelo (cultivares BMX Potência RR e CD 202). Inicialmente, as sementes dos quatro genótipos foram multiplicadas, na safra 2012/2013, em casa de vegetação, na Embrapa Clima Temperado -Estação Experimental Terras Baixas. Após a colheita, as sementes ficaram armazenadas, sob condições controladas, durante cinco meses. Depois desse período, junto à UFPel, se determinou a concentração de compostos fenólicos, potencial antioxidante, antocianinas, carotenóides e lignina dos tegumentos. Também se analisou a estrutura interna destes, por meio de cortes anatômicos, sendo a docume...

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“…A diferença nas tonalidades sugere a diferença de concentração deste composto em função do tempo de extração. Os valores de antocianinas encontrados para a soja preta utilizada neste estudo estão de acordo com os resultados apresentados por Zhang et al (2011) que avaliaram as antocianinas presentes em sessenta variedades de soja preta originárias da China, obtendo valores entre de 98,8 a 2132,5 mg/100g. Quanto a atividade antioxidante os extratos obtidos da soja preta nas extrações de 20 e 30 minutos apresentaram valores de EC 50 entre 0,12 e 0,14 mg/L.…”

Section: Resultsunclassified

ATIVIDADE ANTIRRADICAL LIVRE DE COMPOSTOS BIOATIVOS DE SOJA PRETA (Glycine max L. Merril)

Esteves¹,

Faria-Machado²,

Felberg³

et al. 2015

Anais Do XX Congresso Brasileiro De Engenharia Química

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RESUMO -A soja preta (Glycine max L. Merril), pouco conhecida no ocidente, tem despertado a atenção de pesquisadores e consumidores para seu potencial promotor de saúde devido à presença de antocianinas na casca. Considerando a importância do Brasil na produção mundial de soja, o conhecimento desta cultivar apresenta-se como uma oportunidade de desenvolvimento desta cultura no país. Este trabalho teve como objetivo extrair compostos bioativos de soja preta e estimar sua atividade antioxidante. Os compostos bioativos foram extraídos com solução metanólica 60 % com 1 % de HCl em ultrassom por 20 e 30 minutos em duas etapas, o teor de antocianinas totais foi determinado pela absorbância a 535 nm e a atividade antioxidante estimada por meio do ensaio de DPPH. A extração por 30 minutos foi mais adequada apresentando concentração média de antocianinas de 90,8 mg/100g, tendo o extrato apresentado EC50 de 0,14 mg/L. Os resultados confirmaram o importante potencial antioxidante da soja preta.

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Phenolic Composition and Antioxidant Activity in Seed Coats of 60 Chinese Black Soybean (Glycine max L. Merr.) Varieties

Cited by 135 publications

References 31 publications

Anthocyanin and Isoflavone Contents in Korean Black Soybean Landraces and Their Antioxidant Activities

Anthocyanin and Isoflavone Contents in Korean Black Soybean Landraces and Their Antioxidant Activities

Chemical composition and structural characterization of contrasting colors of soybean seed coats

ATIVIDADE ANTIRRADICAL LIVRE DE COMPOSTOS BIOATIVOS DE SOJA PRETA (Glycine max L. Merril)

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