Phase-contrast X-ray computed tomography of non-formalin fixed biological objects

Takeda, Takeshi; Momose, Atsushi; Wu, Jin; Zeniya, Tsutomu; Yu, Quanwen; Thet-Thet-Lwin,; Itai, Yuji

doi:10.1016/s0168-9002(01)00656-8

Cited by 6 publications

(5 citation statements)

References 11 publications

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“…In recent years, a novel x-ray imaging technique, x-ray phase contrast imaging (XPCI) with contrast based on the refractive index rather than on absorption, has demonstrated its unique forte when dealing with weakly absorbing samples, such as soft tissues and polymers (Momose 1995, Davis et al 1995, Chapman et al 1997, Snigirev et al 1995, Yokosuka et al 2002. Its potential applications in diverse fields, especially in the fields of clinical and material science have been heavily reported in the last 10 years (Keyrilaeinen et al 2005, Rocha et al 2005, Gao et al 1999, Takeda et al 2001, Stevenson et al 2003, Arfelli et al 2003, Tsai et al 2002, Hwu et al 2002, Besseghini et al 2004.…”

Section: Introductionmentioning

confidence: 99%

Application of x-ray phase contrast imaging to microscopic identification of Chinese medicines

Wei

Xiao²,

Liu³

et al. 2005

Phys. Med. Biol.

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In the last decade, x-ray phase contrast imaging (XPCI) has received considerable attention as a novel imaging technique, which has proved to be suitable for weakly absorbing materials such as biomedical samples and polymers. In this paper, the microstructures of traditional Chinese medicines (TCMs), which are used as judging criteria in the identification of TCMs, were investigated by XPCI based on a nano-focus x-ray tube. The results demonstrated that XPCI is a promising new method for the identification of TCMs, with advantages such as nondestructivity, no special sample preparation and suitability for thick samples.

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Section: Introductionmentioning

confidence: 99%

Application of x-ray phase contrast imaging to microscopic identification of Chinese medicines

Wei

Xiao²,

Liu³

et al. 2005

Phys. Med. Biol.

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“…In addition, the high spatial resolution as by micro x-ray imaging leads to the less integrated linear attenuation in target regions, and this also induces the difficulty in detecting the minute density difference of the inner structures on the object. On the other hand, the phase-contrast X-ray imaging technique has high sensitive to depict the inner soft tissue structures of human and animals [6][7][8][9][10][11][12][13], and recently we can observe the biological object with higher spatial resolution by the micro-phase-contrast CT [16,17,25].…”

Section: Discussionmentioning

confidence: 99%

“…Since 1995, the phase-contrast X-ray computed tomography with an X-ray interferometer (phase-contrast CT) has been reported [4,5] and applied to image the histopathological structures of biomedical objects such as various cancers of human [6][7][8], brain [9,10], VX-2 cancer [11,12] and hepatic vessels [13] of small animal. Especially, the phase-contrast CT images can be acquired three dimensionally, so the observation and analysis become much easier in biomedical objects.…”

Section: Introductionmentioning

confidence: 99%

Microphase-contrast x-ray computed tomography for basic biomedical study at SPring-8

Takeda

Lwin

et al. 2004

SPIE Proceedings

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Micro-phase-contrast X-ray computed tomography with an X-ray interferometer (micro-phase-contrast CT) is in operation to obtain high spatial resolution images of less than 0.01 mm at the undulator beam-line 20XU of SPring-8, Japan, and we applied micro-phase-contrast CT to observe the organs of rats and hamsters. The excised kidney and spleen fixed by formalin were imaged. The fine inner-structures such as vessels, glomeruli of kidney and white and red pulps of spleen were visualized clearly about 0.01-mm spatial resolutions without using contrast agent or staining procedure. The results were very similar to those by optical microscopic images with 20-fold magnification. These results suggest that the micro-phase tomography might be a useful tool for various biomedical researches.

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“…Another advantage of interferometric imaging is that x-ray phase maps can be easily obtained using phase retrieval techniques such as the fringe scanning method (Bruning et al 1974) or the Fourier-transform method (Takeda et al 1982). Because of these advantages, x-ray interferometric imaging has been used to reveal structures in rat cerebellum , cancerous rabbit liver , 1996, Takeda et al 2001 and rat nerve (Bonse et al 1997). Human cancerous tissues in breast (Momose et al 1998a, 1998b, liver (Momose et al 1998b, Takeda et al 2000 and kidney (Momose et al 1999, Bonse andBeckmann 2001) have also been observed.…”

Section: Introductionmentioning

confidence: 99%

X-ray phase imaging of biological soft tissue using a direct-sensing x-ray HARP tube camera

et al. 2007

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A HDTV camera having a direct-sensing x-ray high-gain avalanche rushing amorphous photoconductor (HARP) tube was used, for the first time, to acquire x-ray phase maps. The tube can achieve a high sensitivity as a result of the avalanche multiplication process in the HARP target. A beryllium plate, rather than a glass plate, was used as the face plate of the tube to minimize the loss of x-rays due to absorption, and a 15 microm thick HARP target was directly formed on it. In the experiment, the x-ray phase shifts produced by a rat liver were measured using synchrotron x-rays (lambda = 0.0766 nm) and a triple Laue-case (LLL) x-ray interferometer. Interference patterns produced by the sample were observed with the direct-sensing x-ray HARP tube camera. A voltage of 1300 V was applied to the HARP target to give an output signal gain of two. The camera was operated in 1125 scanning-line mode, and real-time images were stored on a workstation at a rate of 30 images/s with an image format of 960 (H) x 1100 (V) pixels. A phase-map image of the sample was successfully obtained using the fringe scanning method and phase unwrapping. The observed phase shifts ranged from 50 degrees to 200 degrees . Trees of blood vessels in the rat liver were clearly depicted without using a contrast agent. The spatial resolution of the x-ray camera was estimated to be better than 35 microm in the vertical direction and 100 microm in the horizontal direction.

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Phase-contrast X-ray computed tomography of non-formalin fixed biological objects

Cited by 6 publications

References 11 publications

Application of x-ray phase contrast imaging to microscopic identification of Chinese medicines

Application of x-ray phase contrast imaging to microscopic identification of Chinese medicines

Microphase-contrast x-ray computed tomography for basic biomedical study at SPring-8

X-ray phase imaging of biological soft tissue using a direct-sensing x-ray HARP tube camera

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