Microphase-contrast x-ray computed tomography for basic biomedical study at SPring-8

Wu, Rongli; Takeda, Takeshi; Lwin, Thet Thet; Koyama, Ichiro; Momose, Atsushi; Fujii, Akiko; Hamaishi, Yoshitaka; Kuroe, Taichi; Yuasa, Tetsuya; Suzuki, Yoshio; Akatsuka, Takao

doi:10.1117/12.559390

Cited by 4 publications

(3 citation statements)

References 23 publications

(35 reference statements)

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“…Li Gang [14] used the best simplest two crystals to investigate the relation between the kidney slices thickness and imaging quality. Wu T [15] obtained rat kidney slices imaging as high resolution of 0.01 mm with an X-ray interferometer. Yoon [16] imaged the normal kidney and carcinoma tissue, he found the images with clear edge enhancement revealed clearly microstructure in thick kidney tissue (more than 700-fold thicker than microscopic images).…”

Section: Jinst 8 C08004mentioning

confidence: 99%

Experimental investigation of mouse kidney aging with SR PCI technology

Yifeng

Zhao

et al. 2013

J. Inst.

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Objective. Basing on the coherence character of the Synchrotron radiation (SR), the mouse kidney study is performed using the propagation-based phase-contrast imaging (PCI) technology which as one approach of the phase contrasts imaging (PCI). The aim of this paper was to visualize the kidney at different ages and evaluate the latent value of aging mechanism with SR phase contrast imaging technology. Methods. The experiments were performed at the BL13W1 line of the SSRF (the Shanghai synchrotron radiation facility), the samples were soaked in 10% formalin solution, the mouse kidneys at different ages were imaged on the shelf in the propagation-based phase-contrast imaging setup and captured with CCD. The captured images were analyzed and compared. Results. When the distance is 50 cm between the samples and imaging plate, good contrast and high resolution were obtained in the propagation-based phase-contrast imaging (PCI), as such renal capsule revealed well, and the resolution reach to 30 micron; there is significant difference in the shape and vessels structures among the mouse kidneys at different age. Conclusion. The PCI is good for the applying of main light element organization imaging, the difference in shape and vessels structure between the young and old mouse kidney maybe indicated at some extent with the propagation-based phase-contrast imaging technology.

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Section: Jinst 8 C08004mentioning

confidence: 99%

Experimental investigation of mouse kidney aging with SR PCI technology

Yifeng

Zhao

et al. 2013

J. Inst.

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“…A few examples are mentioned here in closing the subsection, including damage in composites 143,144 and structures in biological specimens. 126,145 Real time phase radiography of insect respiration produced interesting new insights, 130 and phase microCT, providing the third dimension, will undoubtedly prove very valuable.…”

Section: Phase Contrast Microctmentioning

confidence: 99%

Recent advances in X-ray microtomography applied to materials

Stock

2008

International Materials Reviews

443

208

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This review highlights recent advances in X-ray microcomputed tomography (microCT) as applied to materials, specifically advances made since the first materials microCT review appeared in International Materials Reviews. 1 Improvements in instrumentation are covered, and one focus is microCT using phase (as opposed to absorption) contrast. Instead of grouping studies by disciplines, the reviewed reports are organised by type of application, specifically the study of the spatial distribution of phases, of cellular solids (including static and temporally evolving structures, fibrous network solids, mineralised tissues and biomedical applications), of channel structures, of deformation, fatigue and fracture, of processing and of corrosion and environmental interactions. Metrology applications are covered briefly, and several applications where microCT is combined with position resolved X-ray scattering are described in more detail. The accuracy of microCT reconstructions is discussed before data handling challenges are outlined. The review closes with speculations on the future directions of materials microCT.

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“…24,25 Using the X-ray phase-contrast microtomography system with this crystal X-ray interferometer (phase-contrast microtomography), we depicted the microstructure of kidney and spleen of rat earlier. 25,26 The purpose of this study is to examine the feasibility of phase-contrast microtomography for visualizing microstructures, and to analyze quantitatively the density alteration of microstructures in the kidney of APA hamsters.…”

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confidence: 99%

Imaging renal structures by X-ray phase-contrast microtomography

Takeda

Lwin

et al. 2009

Kidney International

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X-ray crystal interferometer-based X-ray phase-contrast microtomography (phase-contrast microtomography) is able to image microstructures within soft tissue without the use of a contrast agent. Here we determined the feasibility of using this technique in the non-destructive inspection of formalin-fixed kidney tissue from certain hamsters that spontaneously develop mesangial thickening with focal and segmental glomerulosclerosis, and from age-matched Syrian hamsters. We used a triple Laue-case X-ray interferometer with a 40 microm-thick analyzer, a sample cell, and an X-ray charge-coupled-device camera with a 4.34 microm pixel size. Images of glomeruli and tubular structures were similar to those seen using 40-100 x magnification on an optical microscope. In samples from two female glomerulosclerotic hamsters, seven scattered lesions were detected. The wedge-shaped pathological lesions included mild atrophic tubular walls, markedly dilated tubular lumen, high-density glomeruli, and widening of Bowman's space. The microvasculature was distinctly visualized in the specimens without any contrast agents. Hence, phase-contrast microtomography can detect small scattered lesions in diseased kidney tissue and is a powerful auxiliary tool for pre-histological evaluations.

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Microphase-contrast x-ray computed tomography for basic biomedical study at SPring-8

Cited by 4 publications

References 23 publications

Experimental investigation of mouse kidney aging with SR PCI technology

Experimental investigation of mouse kidney aging with SR PCI technology

Recent advances in X-ray microtomography applied to materials

Imaging renal structures by X-ray phase-contrast microtomography

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