Experimental investigation of mouse kidney aging with SR PCI technology

Abstract

Objective. Basing on the coherence character of the Synchrotron radiation (SR), the mouse kidney study is performed using the propagation-based phase-contrast imaging (PCI) technology which as one approach of the phase contrasts imaging (PCI). The aim of this paper was to visualize the kidney at different ages and evaluate the latent value of aging mechanism with SR phase contrast imaging technology. Methods. The experiments were performed at the BL13W1 line of the SSRF (the Shanghai synchrotron radiation facility), the samples were soaked in 10% formalin solution, the mouse kidneys at different ages were imaged on the shelf in the propagation-based phase-contrast imaging setup and captured with CCD. The captured images were analyzed and compared. Results. When the distance is 50 cm between the samples and imaging plate, good contrast and high resolution were obtained in the propagation-based phase-contrast imaging (PCI), as such renal capsule revealed well, and the resolution reach to 30 micron; there is significant difference in the shape and vessels structures among the mouse kidneys at different age. Conclusion. The PCI is good for the applying of main light element organization imaging, the difference in shape and vessels structure between the young and old mouse kidney maybe indicated at some extent with the propagation-based phase-contrast imaging technology.

Metrology and Measurement Systems

“…The advantage of the present process, in real imaging, is that it can be employed on any instrumentation being able to capture inner energies, thermal and mechanical, emitted by human organ during surgery or other explorations. The kidney, as a pivotal organ, basically beahaves in the same way [34]. Once results of thermal and mechanical stress are available, surgeons can work on a database related to specific organs by listing patients' records such age, gender, activities (job), other pathologies, etc.…”