Imaging renal structures by X-ray phase-contrast microtomography

Wu, Jin; Takeda, Takeshi; Lwin, Thet Thet; Momose, Atsushi; Sunaguchi, Naoki; Fukami, Tatsuki; Yuasa, Tetsuya; Akatsuka, Takao

doi:10.1038/ki.2009.42

Cited by 43 publications

(37 citation statements)

References 37 publications

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“…Crystal-interferometer-based phase-contrast imaging techniques have ultra-high sensitivity for phase objects including biological samples, and phase-contrast tomography enables observation of slight density differences in biological soft tissues. In fact, crystal-interferometer-based phase-contrast images can successfully visualize the microstructure of various tissues (Momose et al, 1996;Beckmann et al, 1999;Shinohara et al, 2008;Connor et al, 2009;Wu et al, 2009). However, this system requires highly brilliant X-rays, such as synchrotron radiation (Shinohara et al, 2008;Wu et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…In fact, crystal-interferometer-based phase-contrast images can successfully visualize the microstructure of various tissues (Momose et al, 1996;Beckmann et al, 1999;Shinohara et al, 2008;Connor et al, 2009;Wu et al, 2009). However, this system requires highly brilliant X-rays, such as synchrotron radiation (Shinohara et al, 2008;Wu et al, 2009). On the other hand, an approach using the grating interferometer permits the use of conventional X-ray sources in the field of joint disease (Tanaka et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…X-ray phasecontrast CT imaging techniques are based on the phase shift of X-ray waves that occurs due to refraction and have approximately a thousand-fold higher sensitivity for detecting light elements, such as hydrogen, carbon, nitrogen and oxygen, than conventional X-ray absorption-contrast techniques (Momose et al, 1996). In fact, X-ray phase-contrast CT imaging has successfully visualized the microstructure of normal and pathological tissues including brain (Connor et al, 2009), kidney (Wu et al, 2009), artery (Shinohara et al, 2008), blood vessel (Hu et al, 2012) and eye (Hoshino et al, 2011(Hoshino et al, , 2012. In addition, the phase factor in the complex refractive index, on which this imaging contrast is based, is proportional to mass density (Momose et al, 1996;Hoshino et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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X-ray phase-contrast computed tomography visualizes the microstructure and degradation profile of implanted biodegradable scaffolds after spinal cord injury

Takashima¹,

Hoshino²,

Uesugi³

et al. 2015

J Synchrotron Radiat

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Tissue engineering strategies for spinal cord repair are a primary focus of translational medicine after spinal cord injury (SCI). Many tissue engineering strategies employ three-dimensional scaffolds, which are made of biodegradable materials and have microstructure incorporated with viable cells and bioactive molecules to promote new tissue generation and functional recovery after SCI. It is therefore important to develop an imaging system that visualizes both the microstructure of three-dimensional scaffolds and their degradation process after SCI. Here, X-ray phase-contrast computed tomography imaging based on the Talbot grating interferometer is described and it is shown how it can visualize the polyglycolic acid scaffold, including its microfibres, after implantation into the injured spinal cord. Furthermore, X-ray phase-contrast computed tomography images revealed that degradation occurred from the end to the centre of the braided scaffold in the 28 days after implantation into the injured spinal cord. The present report provides the first demonstration of an imaging technique that visualizes both the microstructure and degradation of biodegradable scaffolds in SCI research. X-ray phase-contrast imaging based on the Talbot grating interferometer is a versatile technique that can be used for a broad range of preclinical applications in tissue engineering strategies.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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X-ray phase-contrast computed tomography visualizes the microstructure and degradation profile of implanted biodegradable scaffolds after spinal cord injury

Takashima¹,

Hoshino²,

Uesugi³

et al. 2015

J Synchrotron Radiat

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“…In a conventional X-ray CT image, the absorption differences among cancer, fibrosis, necrosis, and normal tissues are difficult to detect because the differences in the linear attenuation coefficients of these tissues are very small. As mentioned earlier, XII enables visualization of the inner structures of human cancer specimens (Takeda et al, 2000) and animal cancer specimens (Momose et al, 1996;Takeda et al, 2004d), the brain (Beckmann et al, 1997), and the kidney (Wu et al, 2009) without contrast agents composed of heavy atomic elements. Here, we describe the images of cancer specimens obtained using XII at 35-keV X-ray energy.…”

Section: Formalin-fixed Colon Cancer Specimens From Nude Micementioning

confidence: 99%

Fine Biomedical Imaging Using X-Ray Phase-Sensitive Technique

Yoneyama¹,

Yamada²,

Takeda³

2011

Advanced Biomedical Engineering

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“…[16][17][18] Reports of the application of SR-PCT for neurovascular imaging are limited. 19 In the present study, we used SR-PCT as a high-resolution imaging tool to investigate the 3D angioarchitecture of the normal spinal cord and the alterations associated with acute SCI.…”

Section: Introductionmentioning

confidence: 99%

3D angioarchitecture changes after spinal cord injury in rats using synchrotron radiation phase-contrast tomography

Cao

et al. 2015

Spinal Cord

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Study design: A basic experiment study. Objectives: An understanding of the three-dimensional (3D) angioarchitecture changes that occur after SCI will improve our knowledge of the pathogenesis of SCI and aid in the development of valuable therapeutic strategies to improve its poor outcomes. Our aim was to visualize the normal and traumatized spinal angioarchitecture in 3D using a high-resolution synchrotron radiation phase-contrast tomography (SR-PCT) and evaluate its diagnostic capability. Setting: SCI Center of Xiangya Hospital of Central South University in China. Methods: SR-PCT was used as novel high-resolution imaging tool to detect 3D morphological alterations in spinal cord microvasculature after injury. Results: In a rat model, the morphology of the microvasculature on 2D digital slices was matched with histological findings in both the normal and injured spinal cord. 3D angioarchitecture changes after SCI were successfully obtained via SR-PCT without the use of a contrast agent. Quantitative analysis on 3D images of the injured spinal cord revealed a significant decrease in the number and volume of vascular networks. This was especially relevant to vessels with a diameter o50 μm. Conclusion: The 3D local blood supply to the spinal cord was severely disrupted after the acute violent injury. Our results indicate that the use of SR-PCT may improve our understanding of the pathogenesis of SCI and provide a new approach to the morphological investigation of neurovascular diseases in preclinical research. INTRODUCTIONThe spinal cord microvasculature is a complex and delicate threedimensional (3D) structure in the central nervous system. Under normal physiological conditions, the spinal cord microvasculature enables neural tissue to survive by providing necessary nutrition and maintaining a balanced local microenvironment. 1 Under the pathological process of a spinal cord injury (SCI), acute trauma can severely injure the spinal cord vasculature and result in irreversible damage to neurological function. 2 Revascularization following SCI is a complex process that involves remodeling of the number, connectivity, spatial distribution and direction of blood vessels, all of which affect the functional recovery of the spinal cord from trauma-induced ischemia. 3 Knowledge of the 3D pathologic changes in the spinal cord microvasculature after SCI may aid in the development of valuable therapeutic strategies to improve its poor outcomes. Currently, there is a lack of effective imaging techniques for quantitative evaluation of the microvasculature difference in normal and injured spinal cord models. A number of studies have used 2D and 3D imaging to investigate the vascular response to SCI, but available technology is not sophisticated enough to provide clinically meaningful results. 4 Micro-computed tomography is a powerful 3D imaging tool that is widely used to examine the morphology of various biomedical structures. 5 However, low spatial resolution renders this tool unsuitable for accurate and complete 3D reconst...

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Imaging renal structures by X-ray phase-contrast microtomography

Cited by 43 publications

References 37 publications

X-ray phase-contrast computed tomography visualizes the microstructure and degradation profile of implanted biodegradable scaffolds after spinal cord injury

X-ray phase-contrast computed tomography visualizes the microstructure and degradation profile of implanted biodegradable scaffolds after spinal cord injury

Fine Biomedical Imaging Using X-Ray Phase-Sensitive Technique

3D angioarchitecture changes after spinal cord injury in rats using synchrotron radiation phase-contrast tomography

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