Peroxidase-mimicking nanozyme with surface-dispersed Pt atoms for the colorimetric lateral flow immunoassay of C-reactive protein

Panferov, Vasily G.; Byzova, Nadezhda A.; Zherdev, Anatoly V.; Dzantiev, Boris B.

doi:10.1007/s00604-021-04968-x

Cited by 25 publications

(25 citation statements)

References 48 publications

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“…Figure 4 summarizes the application data and the various types of nanoparticle modifications described below. [74], Pt-nanoflower-type NPs [75], Au@PTNps [76], Au@Ag-PtNPs [77], quantum dots [78][79][80], magnetic beads [81].…”

Section: Immunodiagnostic Systems Based On Nanomaterialsmentioning

confidence: 99%

“…Another approach providing enhanced catalytic activity of nanozymes was to create nanoparticles with an effective dispersion of Pt atoms on their surface owing to a modified synthesis technology [77]. Namely, the synthesis was conducted in three stages: synthesis of gold nanoparticles and the building of a layer of silver on them, followed by the galvanic substitution of PtCl 6 2− for silver.…”

Section: Immunochromatographic Test Systems (Lfia)mentioning

confidence: 99%

“…Figure 4. Useful modifications of nanoparticles for improvement of the analyte detection described in this review: AuNPs[65][66][67][68][69][70][71][72][73], Superspherical AuNPs[74], Pt-nanoflower-type NPs[75], Au@PTNps[76], Au@Ag-PtNPs[77], quantum dots[78][79][80], magnetic beads[81].…”

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confidence: 99%

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Antibodies as Biosensors’ Key Components: State-of-the-Art in Russia 2020–2021

Rudenko

Fursova

Shepelyakovskaya

et al. 2021

Sensors

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The recognition of biomolecules is crucial in key areas such as the timely diagnosis of somatic and infectious diseases, food quality control, and environmental monitoring. This determines the need to develop highly sensitive display devices based on the achievements of modern science and technology, characterized by high selectivity, high speed, low cost, availability, and small size. Such requirements are met by biosensor systems—devices for reagent-free analysis of compounds that consist of a biologically sensitive element (receptor), a transducer, and a working solution. The diversity of biological material and methods for its immobilization on the surface or in the volume of the transducer and the use of nanotechnologies have led to the appearance of an avalanche-like number of different biosensors, which, depending on the type of biologically sensitive element, can be divided into three groups: enzyme, affinity, and cellular/tissue. Affinity biosensors are one of the rapidly developing areas in immunoassay, where the key point is to register the formation of an antigen–antibody complex. This review analyzes the latest work by Russian researchers concerning the production of molecules used in various immunoassay formats as well as new fundamental scientific data obtained as a result of their use.

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Section: Immunodiagnostic Systems Based On Nanomaterialsmentioning

confidence: 99%

Section: Immunochromatographic Test Systems (Lfia)mentioning

confidence: 99%

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Antibodies as Biosensors’ Key Components: State-of-the-Art in Russia 2020–2021

Rudenko

Fursova

Shepelyakovskaya

et al. 2021

Sensors

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“…With the development of nanotechnology, nanomaterial-based enzyme mimics (nanozyme) are widely used as alternatives to natural enzymes because of their attractive merits of outstanding tolerance in harsh environments, easy large-scale production, and low cost. The reported enzyme-like activities include the peroxidase-like activity [ 23 ], superoxide dismutase-like activity [ 7 ], catalase-like activity [ 17 ], and so on. The unique characteristics of nanomaterials and the synergistic effect of enzyme-like activities, making nanozymes potential applications in wide fields including biosensing [ 34 ], food industry, pharmaceutical processes, and environmental treatment [ 36 ].…”

Section: Introductionmentioning

confidence: 99%

In situ synthesis of Co-doped MoS2 nanosheet for enhanced mimicking peroxidase activity

Zhang

Tang

et al. 2022

J Mater Sci

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To enhance the catalytic activity of two-dimensional layered materials as versatile materials, the modification of transition metal dichalcogenide nanosheets such as MoS 2 by doping with heteroatoms has drawn great interests. However, few reports are available on the study of the enzyme-like activity of doped MoS 2 . In this study, a facile in situ hydrothermal method for the preparation of various ultrathin transition metals (Fe, Cu, Co, Mn, and Ni) doped MoS 2 nanosheets has been reported. Through the density functional theory (DFT) and steady-state kinetic analysis, the Co-doped MoS 2 nanosheets exhibited the highest peroxidase-like catalytic activity among them. Furthermore, a typical colorimetric assay for H 2 O 2 was presented based on the catalytic oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to a blue product (oxTMB) by Co-MoS 2 . The proposed colorimetric method showed excellent tolerance under extreme conditions and a broad linear range from 0.0005 to 25 mM for H 2 O 2 determination. Concerning the practical application, in situ detection of H 2 O 2 generated from SiHa cells was also fulfilled, fully confirming the great practicability of the proposed method in biosensing fields. Supplementary Information The online version contains supplementary material available at 10.1007/s10853-022-07201-z.

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“…Moreover, mesoporous silica shells allow molecular diffusion and transport of the reactants in catalysis between the exterior and interior environments. − Additionally, the easy modification properties of mesoporous silica shells render coated nanoparticles highly promising as ideal carriers for high-efficiency immobilization of functional species such as recognition antibodies, which allows sensitive diagnosis . On the other hand, noble metal nanomaterials (i.e., silver, gold, palladium, and platinum) have fascinated scientists for over a century due to their unique performance in catalysis, electronics, and biology. , Additionally, recently, small Pt nanoparticles have showed multiple enzyme-like activities under different conditions, for example, oxidase-like, peroxidase-like, superoxide dismutase (SOD)-like, and catalase-like activities. − Nowadays, platinum-based assays have been developed for ultrasensitive detection of small molecules, ions, nucleic acids, and proteins. − It is generally accepted that the characteristics of the catalytic activity basically depend on the size, microstructure, and surface state of Pt nanoparticles. − …”

Section: Introductionmentioning

confidence: 99%

Hollow Pt Nanocage@Mesoporous SiO₂ Nanoreactors as a Nanozyme for Colorimetric Immunoassays of Viral Diagnosis

Lin

Zong

Xie³

et al. 2021

ACS Appl. Nano Mater.

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A facile and general strategy has been employed to fabricate a “nanoreactor” consisting of a hollow Pt nanocage-encapsulated mesoporous SiO2 shell. The hollow composite, which contained highly dispersed catalytic Pt nanoparticles inside the cavity enclosed by the mesoporous SiO2 shell, was considered an ideal framework structure for a nanoreactor that efficiently catalyzes substrate molecules with enhanced activity. The designed SiO2 shell can not only maintain active Pt nanoparticle resistance to harsh environments but also isolate the molecular recognition sites from the surface of Pt nanoparticles, thus retaining their catalytic activity. In addition, the certain space confinement effect aroused by the hollow structure led to a higher catalytic activity. Based on their catalytic activity, these nanoreactors have been demonstrated to function as biosensors for highly sensitive detection of measles-specific IgM antibodies in capture enzyme-linked immunosorbent assays. The detection limit of the prepared biosensor was as low as 1 ng/mL, 3 orders of magnitude lower than that of the conventional immunoassay. The specific catalytic properties of this nanoreactor could have great potential applications that include catalysis, biological labeling, and bioassays.

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Peroxidase-mimicking nanozyme with surface-dispersed Pt atoms for the colorimetric lateral flow immunoassay of C-reactive protein

Cited by 25 publications

References 48 publications

Antibodies as Biosensors’ Key Components: State-of-the-Art in Russia 2020–2021

Antibodies as Biosensors’ Key Components: State-of-the-Art in Russia 2020–2021

In situ synthesis of Co-doped MoS2 nanosheet for enhanced mimicking peroxidase activity

Hollow Pt Nanocage@Mesoporous SiO₂ Nanoreactors as a Nanozyme for Colorimetric Immunoassays of Viral Diagnosis

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Peroxidase-mimicking nanozyme with surface-dispersed Pt atoms for the colorimetric lateral flow immunoassay of C-reactive protein

Cited by 25 publications

References 48 publications

Antibodies as Biosensors’ Key Components: State-of-the-Art in Russia 2020–2021

Antibodies as Biosensors’ Key Components: State-of-the-Art in Russia 2020–2021

In situ synthesis of Co-doped MoS2 nanosheet for enhanced mimicking peroxidase activity

Hollow Pt Nanocage@Mesoporous SiO2 Nanoreactors as a Nanozyme for Colorimetric Immunoassays of Viral Diagnosis

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Product

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Hollow Pt Nanocage@Mesoporous SiO₂ Nanoreactors as a Nanozyme for Colorimetric Immunoassays of Viral Diagnosis