Perlucidibaca piscinae gen. nov., sp. nov., a freshwater bacterium belonging to the family Moraxellaceae

Abstract

Perlucidibaca piscinae gen. nov., sp. nov., a freshwater bacterium belonging to the family Moraxellaceae Jaeho Song, Yoe-Jin Choo and Jang-Cheon Cho Correspondence Jang-Cheon Cho chojc@inha.ac.kr Division of Biology and Ocean Sciences, Inha University, Incheon 402-751, Republic of Korea A freshwater bacterium, designated IMCC1704 T , was isolated from a eutrophic pond. The strain was Gram-negative, oxidase-positive, catalase-negative, chemoheterotrophic and facultatively aerobic with cells that were motile rods with a single polar flagellum. Based on 16S rRNA gene sequence similarity analyses, the novel strain was most closely related to the genera Alkanindiges (91.7 %), Acinetobacter (89.0-91.2 %), Moraxella (87.9-90.1 %), Psychrobacter (87.2-89.5 %) and Enhydrobacter (87.8 %). Phylogenetic trees generated using 16S rRNA gene sequences showed that the novel isolate belonged to the family Moraxellaceae of the class Gammaproteobacteria and formed a distinct phyletic lineage within the family. The DNA G+C content of the strain was 63.1 mol% and the predominant constituents of the cellular fatty acids were C 16 : 1 v7c and/or iso-C 15 : 0 2-OH (21.2 %), C 18 : 1 v7c (12.8 %) and C 12 : 0 3-OH (12.3 %). These chemotaxonomic properties, together with several phenotypic characteristics, differentiated the novel strain from other members of the family Moraxellaceae. From the taxonomic data, which revealed the distant relationship of the new strain to the related genera, the strain should be classified as a novel genus and species in the family Moraxellaceae, for which the name Perlucidibaca piscinae gen. nov., sp. nov. is proposed. The type strain of Perlucidibaca piscinae sp. nov. is IMCC1704 T (5KCCM 42363

Paraperlucidibaca baekdonensis gen. nov., sp. nov., isolated from seawater

“…The fatty acid profile of strain RL-2 T was essentially similar to that of Perlucidibaca piscinae IMCC1704 T , even though there were differences in the proportions of some components (Table 2). However, the predominant ubiquinone (Q-11) of strain RL-2 T was different from those of other members of the family Moraxellaceae as well as from Perlucidibaca piscinae IMCC1704 T , which contain Q-8 or Q-9 as the predominant ubiquinone (Song et al, 2008). Strain RL-2 T could also be differentiated from Perlucidibaca piscinae IMCC1704 T based on differences in several phenotypic properties, including motility, optimal growth temperature, NaCl tolerance, anaerobic growth and nitrate reduction, as shown in Table 1.…”

“…The family initially consisted of the genera Moraxella (Lwoff, 1939), Acinetobacter (Brisou & Prévot, 1954) and Psychrobacter (Juni & Heym, 1986). Three other genera of the family were subsequently described, namely Enhydrobacter (Staley et al, 1987), Alkanindiges (Bogan et al, 2003) and Perlucidibaca (Song et al, 2008). The genus Perlucidibaca was distinguished from other members of the family Moraxellaceae on the basis of high DNA G+C content and differences in fatty acid compositions and several phenotypic properties, and was proposed based on the description of a single recognized species, Perlucidibaca piscinae (Song et al, 2008).…”

“…Three other genera of the family were subsequently described, namely Enhydrobacter (Staley et al, 1987), Alkanindiges (Bogan et al, 2003) and Perlucidibaca (Song et al, 2008). The genus Perlucidibaca was distinguished from other members of the family Moraxellaceae on the basis of high DNA G+C content and differences in fatty acid compositions and several phenotypic properties, and was proposed based on the description of a single recognized species, Perlucidibaca piscinae (Song et al, 2008). In this study, we describe a novel bacterial strain, designated RL-2 T , which was isolated from seawater obtained from Baekdo Harbor, East Sea, Korea.…”

“…Strains: 1, RL-2 T (grown for 6 days on R2A agar at 20 u C); 2, Perlucidibaca piscinae IMCC1704 T (6 days on R2A agar at 20 u C; data from this study); 3, Perlucidibaca piscinae IMCC1704 T (3 days on R2A agar at 30 uC; data from Song et al, 2008). Fatty acids that represented ,0.5 % in all columns were omitted.…”

“…Strain RL-2 T exhibited highest 16S rRNA gene sequence Table 1. Differential phenotypic characteristics between strain RL-2 T and Perlucidibaca piscinae IMCC1704 T Data for Perlucidibaca piscinae IMCC1704 T are from Song et al (2008) and this study. W, Weakly positive reaction.…”