Paraperlucidibaca baekdonensis gen. nov., sp. nov., isolated from seawater

Abstract

A Gram-negative, non-spore-forming, rod-shaped bacterial strain, RL-2 T , was isolated from seawater of the East Sea in Korea and was subjected to a polyphasic taxonomic study. Strain RL-2 T grew optimally at pH 7.5-8.0, at 20 6C and in the absence of NaCl. Phylogenetic trees based on 16S rRNA gene sequence analysis showed that strain RL-2 T forms a cluster with Perlucidibaca piscinae IMCC1704 T and various uncultured and unidentified gammaproteobacteria. Strain RL-2 T exhibited 16S rRNA gene sequence similarity values of 96.1 % to Perlucidibaca piscinae IMCC1704 T and 93.7-99.7 % to the uncultured bacterial clones belonging to the cluster and an unidentified gammaproteobacterium. The fatty acid profile of strain RL-2 T was similar to that of Perlucidibaca piscinae IMCC1704 T , but the predominant ubiquinone type (Q-11) of strain RL-2 T was different from that (Q-8) of Perlucidibaca piscinae IMCC1704 T . The DNA G+C content of strain RL-2 T was 61.3 mol%. On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain RL-2 T is considered to represent a novel species of a new genus in the family Moraxellaceae, for which the name Paraperlucidibaca baekdonensis gen. nov., sp. nov. is proposed. The type strain of Paraperlucidibaca baekdonensis is RL-2 T (5KCTC 23145 T 5CCUG 59307 T ).

Cavicella subterranea gen. nov., sp. nov., isolated from a deep mineral-water aquifer, and emended description of the species Perlucidibaca piscinae

“…The type strain of Perlucidibaca piscinae as well as strain W2.09-231 T possessed similar fatty acid types dominated by straight-chain monounsaturated fatty acids (), but there were large differences in the amounts of several fatty acids that allowed differentiation of the two organisms. The G+C content of the DNA of strain W2.09.231 T was 62.0 mol%, which is similar to the content found in Perlucidibaca piscinae and Paraperlucidibaca baekdonensis (Oh et al , 2011) but 10–20 mol% higher than that of other members of the family Moraxellaceae .…”

“…The family Moraxellaceae currently comprises the species of the genera Moraxella (Lwoff, 1939), Acinetobacter (Brisou & Prevot, 1954), Psychrobacter (Juni & Heym, 1986), Enhydrobacter (Staley et al , 1987) Alkanindiges (Bogan et al , 2003), Branhamella (Catlin, 1970), Perlucidibaca (Song et al , 2008) and Paraperlucidibaca (Oh et al , 2011). The members of the family Moraxellaceae are common colonizers of a variety of environments such as mucosal membranes, the skin of humans and animals (mainly by species of the genera Moraxella and Acinetobacter ) as well as marine, freshwater, soil and food-related habitats.…”

“…The phylogenetic dendrograms ( and Fig. S1 available in the online Supplementary Material) reconstructed by the neighbour-joining and maximum-likelihood methods using the arb software package showed the sequences to have the highest pairwise similarity to the species Perlucidibaca piscinae (94.5 %), Paraperlucidibaca baekdonensis (93.1 %) and Paraperlucidibaca wandonensis (92.8 %) (Song et al , 2008; Oh et al , 2011; Yoon et al , 2013). Other species of the family Moraxellaceae showed 16S rRNA gene sequence similarity values below 89.8 % to strain W2.09-231 T .…”

Uncoupling of Bacterial and Terrigenous Dissolved Organic Matter Dynamics in Decomposition Experiments

“…A shift towards the dominance of a Paraperlucidibaca phylotype was not observed in the freshwater control mesocosms (cRW), although this bacterium should be able to grow in freshwater conditions [71]. However, the initial BCC in cRW differed from that of the mesohaline mesocosms (Fig.…”

Enhancing Electricity Generation Using a Laccase-Based Microbial Fuel Cell with Yeast Galactomyces reessii on the Cathode

“…On the other hand, the bacterial Moraxellaceae sp. was found in the broad range of sources, such as wastewater treatment plant sludge, lake sediment [16], activated sludge in a continuous up-flow reactor [17], and sea water [18], whereas the cyanobacteria Kamptonema sp. were found spread in wet soil, plant bark, and stream sand [19].…”