Perfil sérico proteico de vacas Holandesas no período de transição

Abstract

RESUMOO objetivo deste trabalho foi avaliar, de forma longitudinal, o perfil sérico proteico de 13 vacas Holandesas durante o período de transição. Amostras de sangue (n=78) foram coletadas semanalmente, da segunda semana pré-parto (M-2) até a terceira semana pós-parto (M3), para determinação do perfil sérico proteico, por meio de teste bioquímico (proteínas séricas totais -PT) e eletroforese em gel de poliacrilamida SDS-PAGE, para as outras proteínas analisadas. Os valores de PT diminuíram de forma gradativa (P=0,000) de M-2 (6,4g/dL) a M0 (6,2g/dL), aumentando nos momentos subsequentes (M3=7,3g/dL). As concentrações da IgG de cadeia pesada (M-2=919,4; M-1=1074,5mg/dL) e de cadeia leve (M-2=393,9; M-1=466,7mg/dL) foram menores no pré-parto em relação ao pós-parto (M1=1283,3; M2=1374,2 e M3=1630,3 mg/dL para IgG pesada e M1=463,4; M2=573,7; M3=651,8mg/dL para IgG leve). Para a IgA, houve diminuição nos valores (P=0,001), de M-2 (51,9mg/dL) a M1 (34,0mg/dL), e aumento em M2 (45,4g/dL) e M3 (62,6g/dL). Os valores de haptoglobina (Hp) e ceruloplasmina (Cp) aumentaram (P=0,000) de M-2 (Hp=16,6mg/dL; Cp=8,6mg/dL) a M3 (Hp=60,9mg/dL; Cp=127,1mg/dL). A albumina apresentou ligeiras variações durante o período de transição (P=0,000), enquanto a transferrina sérica (P=0,101) e a glicoproteína ácida (P=0,105) foram estáveis. O escore de condição corporal (ECC) também foi analisado durante o período de transição, verificando-se diferença (P=0,003) entre M-2 (ECC=4,0) e M1 (ECC=3,0). Foi relatada ainda a ocorrência de distocias (4/13), retenção de placenta (1/13) e hipocalcemia (1/13) no dia da parição (M0) e infecções uterinas (5/13) e cetose (1/13) ocorridas no pós-parto. Concluiu-se, portanto, que houve aumento nas concentrações séricas de Hp e Cp e diminuição nos valores de imunoglobulina e transferrina em vacas Holandesas no período de transição, relacionados às doenças ocorridas nesse período e ao elevado ECC, promovendo modificações metabólicas e imunossupressão.Palavras-chave: inflamação, proteínas, periparto, bovinos  (M-2=393.9; M-1=466.7mg/dL) were lower in pre-calving compared to post calving (M1= 1,283.3; M2=1,374.2 and M3=1,630.3 mg/dL for the heavy chain, and M1=463.4; M2=573.7 and M3=651,8 ABSTRACT The aim of this study was to evaluate longitudinally the serum protein profile of 13 Holstein cows during the transition period. Blood samples (n=78) were taken weekly, from the second week before parturition (M-2) to the third week after parturition (M3) for determination of serum protein profile by biochemical tests (total serum protein -PT) and SDS-PAGE electrophoresis, for the other proteins analyzed. PT values decreased gradually (P = 0.000) from M-2 (6.4g/dL) to M0 (6.2g/dL), increasing in subsequent moments (M3 = 7.3g/dL). The concentrations of the heavy chain (M-2=919.4; M-1=1074.5mg/dL) and the light chain of IgG

Cardiac biomarkers and blood metabolites in cows with clinical ketosis

“…The globulin concentration and the albumin: globulin (A/G) ratio were not significantly different between the groups, and the observed hyperglobulinemia may be due to the higher synthesis of acute-phase proteins and immunoglobulins (ALLISON, 2015a). Reis et al (2016) and Saut et al (2009) observed that positive acute-phase proteins increased significantly in dairy cows in diseases that occur in the perinatal and postnatal periods.…”

“…However, hypoalbuminemia occurred in both groups, which may be justified by the transition period in which the animals were found. The serum albumin concentration is decreased in the first month after calving as a result of the expansion of the plasma volume for milk production, loss of the uterine lumen during uterine involution, negative acutephase response induced by inflammatory processes in diseases that occur in the perinatal and postnatal periods, and protein catabolism resulting from NEB (CONSTABLE et al, 2017;REIS et al, 2016).…”

Hemogasometry, metabolic profile and acute-phase proteins in primiparous Holstein cows supplemented with butafosfana associated or not of cyanocobalamin

“…Acute-phase proteins have been widely used as biomarkers that can assist in the determination of inflammatory and immunological conditions in the transition period (Eckersall and Bell 2010). Changes in these proteins are associated with several diseases such as uterine infections (Schneider et al 2013), reduction in conception rate (Nightingale et al 2015) and fatty liver and chronic liver damage (Bionaz et al 2007) and can therefore be used to obtain an early diagnosis of the immune status of the animal (Reis et al 2016). The association of butafofan and cyanocobalamin, favouring energy metabolism, may improve immunity in the animal by reducing NEFA levels (Pereira et al 2013).…”