Cardiac biomarkers and blood metabolites in cows with clinical ketosis

Soares, Gliére Silmara Leite; Ribeiro, Ana Clara Sarzedas; Cajueiro, Jobson Filipe de Paula; Souto, Rodolfo José Cavalcanti; Filho, Emanuel Felipe de Oliveira; Soares, Pierre Castro; Mendonça, Carla Lopes de; Afonso, José Augusto Bastos

doi:10.5433/1679-0359.2019v40n6supl3p3525

Cited by 9 publications

(14 citation statements)

References 57 publications

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“…In this clinical condition, hyperglycemia may be associated with decreased pancreatic juice flow and impaired blood circulation in the pancreatic parenchyma, due to alterations in the positioning of the duodenal segment and omentum, which occur during organ displacement [38]. However, the hyperglycemic state can also be justified by the increase in serum cortisol concentrations, induced by the condition of metabolic stress resulting from the disease, as occurs in cases of clinical ketosis [30]. In the current study, animals with RDA presented much higher mean Table 1.…”

Section: Discussionmentioning

confidence: 99%

Cardiac, Energy and Hormonal Blood Markers, and Lactatemia in Cows with Displaced Abomasum

Ribeiro

Soares

Coutinho

et al. 2020

Acta Scientiae. Vet.

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Background: Displaced abomasum (DA) is a common and economically important disorder that affects dairy cattle. Nutritional factors and adaptive responses that occur in the peripartum play a central role in the pathogenesis. The measurement of blood metabolites represents a useful tool for monitoring and prognostic determination in affected animals. Therefore, the objective was to evaluate cardiac, energy and hormonal blood markers, lactatemia, and insulin sensitivity in cows diagnosed with right displaced abomasum (RDA) and left displaced abomasum (LDA), comparing them with each other.Materials, Methods & Results: Nineteen cases of abomasum displacement in cows were studied, including 9 cases of RDA and 10 cases of LDA. The diagnosis was established by means of physical examination and measurement of the concentration of chlorides in the ruminal fluid (> 30mEq/L). After diagnosis, clinical-surgical therapeutic management was instituted. At the time of diagnosis (M1) and at the resolution of the case (M2), blood samples were collected to assess the variables: non-esterified fatty acids (NEFA), beta hydroxybutyrate (βHB), L-lactate, creatine kinase (CK), creatine kinase MB (CK-MB), cardiac troponin I (cTnI), lactate dehydrogenase (LDH), glucose, insulin, and cortisol. In addition, insulin sensitivity was estimated using the Revised Quantitative Insulin Sensitivity Check Index (RQUICKI) and RQUICKI-βHB. The means of the variables were compared, separating the effects of groups (RDA and LDA) and moments (M1 and M2), at the level of 5% probability. The concentrations of NEFA, CK-MB, L-lactate, glucose, insulin, and cortisol were higher at M1 and the RQUICKI and RQUICKI-βHB indices were lower at this moment. L-lactate, CK, and CK-MB were higher in the RDA group, while cTnI, βHB, and LDH did not present a group or moment effect. Cardiac markers correlated with the energy profile metabolites, L-lactate, and cortisol.Discussion: The high concentrations of NEFA at M1 reflected the condition of negative energy balance. βHB concentrations were stable, that may be related to the number of days postpartum in which the animals were diagnosed. The hyperglycemic condition and the increase in serum cortisol concentrations found at M1 can be induced by the condition of metabolic stress resulting from the disease. Hyperinsulinemia were recorded in the present study could be an important factor related to the pathogenesis of DA, since there seems to be a correlation between hyperinsulinemia and decreased abomasal emptying rate. The RQUICKI and RQUICKI-βHB indices was significantly lower at M1, which may indicate lower sensitivity of peripheral tissues to insulin at this time. Changes in serum activity of LDH and CK may result from tissue damage due to organ displacement, in addition to damage associated with surgery and the administration of injectable drugs, mainly intramuscularly. The elevation in plasma L-lactate at M1 and in the RDA group may be associated with abomasal hypoperfusion. The high positive correlations found between L-lactate and the variables glucose, insulin, and cortisol reinforcing the association between the concentration of L-lactate and the moment of greatest stress. The increase in cardiac biomarkers may be related to the occurrence of ischemia/reperfusion injury in the abomasum, which involves oxidative stress and the production of inflammatory mediators. The hyperglycemic condition and the higher concentrations of NEFA can also contribute to the occurrence of myocardial injury. The correlations found between cardiac biomarkers and plasma L-lactate, strengthen the idea that there is a relationship between L-lactate and myocardial injury. In this sense, the measurement of blood concentrations of cTnI, CK-MB, and L-lactate could contribute as severity markers and prognosis indicators in cattle with DA.

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Section: Discussionmentioning

confidence: 99%

Cardiac, Energy and Hormonal Blood Markers, and Lactatemia in Cows with Displaced Abomasum

Ribeiro

Soares

Coutinho

et al. 2020

Acta Scientiae. Vet.

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“…Inflammatory situations also occur in other pregnancy‐related disorders (Yildiz et al., 2024). Clinical signs and serum biochemical profile are important in the diagnosis of the clinical PT (Soares et al., 2019).…”

Section: Introductionmentioning

confidence: 99%

“…Clinical signs and serum biochemical profile are important in the diagnosis of the clinical PT (Soares et al, 2019).…”

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confidence: 99%

Comparison of blood ΒHBA measurement devices for diagnosis of subclinical pregnancy toxaemia in sheep: A field study

Turgut,

Koca,

Ünver

2024

Reprod Domestic Animals

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Pregnancy toxaemia is a serious disease that occurs during the last trimester of pregnancy in sheep. Yet, in most cases, the disease may have a subclinical course. This study was aimed at comparing blood βHBA measurement devices for diagnosis of subclinical pregnancy toxaemia in late pregnant sheep. In the study, a total of 50 blood samples were collected from Romanov (n = 30) and cross‐bred Hamdani (n = 20) sheep. Blood βHBA levels were measured using TaiDoc TD‐4235 and CentriVet βHBA hand‐held meter. Randox βHBA (Ranbut) assay was used as a reference laboratory method to compare hand‐held meters. βHBA value of 0.8 mmol/L was set as the cut‐off value for diagnosis of subclinical pregnancy toxaemia. Statistical analyses were carried out using Minitab 21 and Jamovi software. In the study, the correlation of Randox‐TaiDoc TD‐4235 and Randox‐CentriVet was .822 (p < .001) and .728 (p < .001), respectively. Based on the Ranbut assay, nine ewes out of 50 were diagnosed with subclinical pregnancy toxaemia. Specificity (detection of healthy ewes) and sensitivity (detection of ewes with subclinical pregnancy toxaemia) for TaiDoc TD‐4235 and CentriVet hand‐held meters were 100%, 77.8%, and 100%, 66.7%, respectively. In the receiver operating characteristic (ROC) analysis, areas under the ROC curve (AUC) were 0.976 and 0.920 for TaiDoc and CentriVet, respectively. Bland–Altman analysis revealed a bias of 0.092 mmol/L for TaiDoc and a bias of 0.132 mmol/L for CentriVet. TaiDoc hand‐held meter shows a better correlation with the Randox Ranbut assay and greater sensitivity compared to the CentriVet hand‐held meter. In conclusion, both TaiDoc and CentriVet hand‐held meters can be securely used in the diagnosis of subclinical pregnancy toxaemia in sheep. For these reasons, subclinical pregnancy toxaemia and these devices will be evaluated within the scope of herd management programme in the sheep industry. It should also be taken into account that these conditions will affect the future fertility of the mother and offspring.

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“…The association of complementary exams such as laboratory tests (hematological, biochemical and cavitary effusions) has been an important aid in the early diagnosis of the disease (Silva, 2011;Santos et al, 2020). The use of cardiac function markers such as cardiac troponin I (cTnI) and myocardial band creatine kinase (CK-MB) in ruminants has been well validated in bovines (Soares et al, 2019), goats (Ribeiro et al, 2020;Souza et al, 2020) and ewes (Souza et al, 2019), as well as in cases of traumatic pericarditis due to direct cardiac injury or in secondary involvement in cases of traumatic reticuloperitonitis (Gune et al, 2008;Attia, 2016).…”

Section: Introductionmentioning

confidence: 99%

Systemic implications of metallic foreign body syndrome in dairy cattle

Assis

Souza

Soares

et al. 2021

RSD

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This study aimed to evaluate the systemic implications of 37 cattle with traumatic reticulitis, evaluating clinical, laboratory, ultrasonographic and necroscopic changes. A clinical, laboratory and ultrasonographic examination was performed, and the animals were distributed in reticuloperitonitis (GI; n=21) and reticulopericarditis (GII; n=16) traumatic, based on necropsy findings. Blood samples were taken for haematological tests, serum total protein, albumin, globulin, gamma glutamyltransferase (GGT), aspartate aminotransferase (AST), glutamate dehydrogenase (GLDH), creatinine, urea, cortisol, creatine kinase (CK), creatine kinase-MB (CK-MB), cardiac troponin-I (cTn-I), plasma glucose and L-lactate. The abdominocentesis was productive in eight GI (n=8) and seven GII (n=7) animals, allowing the evaluation of physical, cytological and biochemical characteristics of the peritoneal fluid as a total protein, albumin, GLDH, AST, GGT, glucose and L-lactate. Changes in behavior, appetite, dehydration and temperature were observed, most expressive in GII. Hematology showed neutrophilic leukocytosis with regenerative left shift and hyperfibrinogenemia in both groups. Increased globulin, L-lactate concentration and serum GGT, GLDH, CK and CK-MB activity were observed, as well as significant elevation of cTnI (p=0.0190) in GII. In the peritoneal fluid exudate was observed in both groups and a higher concentration of L-lactate in relation to plasma. Ultrasound revealed retocular, cardiac, hepatic and splenic abnormalities. The anatomopathological lesions confirmed the ultrasound findings of both groups. The understanding of the syndrome helps in the diagnosis, as well as the adoption of preventive measures, minimizing the economic impact caused to the dairy cattle breeding.

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Cardiac biomarkers and blood metabolites in cows with clinical ketosis

Cited by 9 publications

References 57 publications

Cardiac, Energy and Hormonal Blood Markers, and Lactatemia in Cows with Displaced Abomasum

Cardiac, Energy and Hormonal Blood Markers, and Lactatemia in Cows with Displaced Abomasum

Comparison of blood ΒHBA measurement devices for diagnosis of subclinical pregnancy toxaemia in sheep: A field study

Systemic implications of metallic foreign body syndrome in dairy cattle

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