BackgroundsThe maxillary anterior teeth play a crucial role in smile aesthetics. As for maxillary lateral incisors, most studies concentrated on its size, incisor edge level, inclination, et al. However, the aesthetic effect of lateral incisor movement in spatial position has not been studied yet. Therefore, the purpose of this study was to explore the influence of labiolingual position of maxillary lateral incisors on the aesthetic perception of orthodontists and laypersons and different gender groups.MethodsA photograph of a male's smile was selected. iOrtho7.0 software (Time Angel, Wuxi, China) was used to edit the three dimensional (3D) dental models. By referring to the 3D models, the photograph was digitally processed to create seven images with different labiolingual positions of the maxillary lateral incisors in 0.5 mm increments (+ indicates labial movement and -indicates lingual movement). Eighty-six orthodontists and one hundred sixty-one laypersons were asked to evaluate images using a visual analog scale. Data were analyzed by Student t-test and One-way analysis of variance with post hoc test.ResultsThere was no significant difference between the perception of males and females (P > 0.05). Orthodontists assigned lower scores to all images than laypersons. +1.5 mm was considered as the least attractive smile by orthodontists while + 1.5 mm and − 1.5 mm were regarded as the least attractive ones by laypersons. 0 mm was rated the most attractive by all judges (P < 0.05). Between the 0 mm and − 0.5 mm, laypersons observed the difference but orthodontists did not.ConclusionsThe labiolingual position of maxillary lateral incisors does affect the perception of smile esthetics. Although orthodontists gave lower score to each image than laypersons.