Pengaruh Pemberian Kefir Susu Sapi Terhadap Kadar Kolesterol LDL Tikus Jantan Sprague Dawley Hiperkolesterolemia

Abstract

Background: Hypercholesterolemia is a main risk factor of cardiovascular disease that remains the higher cause of deaths in the world. Milk kefir contains lactic acid bacteria which can reduce LDL cholesterol. The objective of this study was to determine the effect of milk kefir on LDL cholesterol levels in male Sprague dawley hypercholesterolemia rats. Methods: This research was true-experimental using pre-post test with randomized control group design. Subjects were male Sprague dawley rats, inducted hypercholesterolemia, given milk kefir diet using 1,5 ml, 2 ml, and 3 ml dosage for 15 days. The LDL cholesterol levels were measured by direct method using homogenous enzymatic LDL cholesterol. Normality of the data were tested by Shapiro Wilk test. Data were analyzed by Wilcoxon test and Kruskall-Wallis continued by Mann-Whitney test. Result: The study revealed that milk kefir of dosage 2 ml/day decreased LDL cholesterol significantly (p<0,05) from 196,57 ± 84,11 mg/dl to 38,95 ± 18,22 mg/dl and also for 3 ml/day decreased LDL cholesterol significantly (p<0,05) from 100,14 ± 36,20 mg/dl to 26,91 ± 9,93 mg/dl. Other dosage of 1,5 ml/day is not significantly decrease LDL cholesterol level. Conclusion: Milk kefir able to reduce LDL cholesterol levels in all groups. By giving mik kefir 2 ml/day and 3ml/day during 15 days able to reduce LDL cholesterol levels 80,18% and 73,12%.

Antihypercholesterolemic Power of Red Dragon Fruit (Hylocereus polyrhizus) Peel Extract

“…The preparation of cow brain suspension was consistent with Pratama and Probosari (2012). 21 Furthermore, 13 g of steamed and mashed cow brain was placed in a beaker and dissolved in 13 ml of distilled water. 0.13 g of cholic acid was then added and stirred until homogenous for 60 days.…”

“…Mice were induced by cow brain suspension at a dose of 0.28 ml/20 g body weight/day for 60 consecutive days. 21 Testing the effects of red dragon fruit peel extract All experimental animals were adapted for 7 days and randomly divided into 4 treatment groups consisting of 6 mice, respectively. Before blood sampling, all mice fasted for ±15 hours, and then blood was collected through the orbital sinus.…”

PENGARUH PEMBERIAN YOGHURT DAN SOYGHURT SINBIOTIK KAYU MANIS (Cinnamomum burmanii) TERHADAP KADAR TRIGLISERIDA DAN TOTAL KOLESTEROL PADA TIKUS PRA-SINDROM METABOLIK

“…Bakteri asam laktat dapat menurunkan kadar kolesterol secara langsung melalui mekanisme asimilasi kolesterol dan secara tidak langsung melalui mekanisme dekonjugasi garam empedu. 5,6,7 Penelitian menunjukkan yoghurt dapat menurunkan kadar kolesterol serum pada tikus dengan hiperlipidemia dan hiperkolesterolemia. 8,9 Bakteri probiotik pada yoghurt dapat mengikat trigliserida dan kolesterol dalam darah sehingga tidak diabsorbsi oleh usus yang mengakibatkan kadar trigliserida dan kolesterol tubuh menurun.…”

“…Fermentasi susu menggunakan bakteri asam laktat dapat menurunkan kadar kolesterol secara langsung melalui mekanisme asimilasi kolesterol dan secara tidak langsung melalui mekanisme dekonjugasi garam empedu. 5,6,7 Penyebab lainnya penurunan kelompok P2 lebih tinggi dibandingkan P1 yaitu protein kacang kedelai pada soyghurt dapat meningkatkan enzim lipoprotein lipase atau LPL, merupakan enzim yang berfungsi menghidrolisis trigliserida menjadi asam lemak bebas dan gliserol. Hal ini menyebabkan kadar trigliserida dalam tubuh pun menurun.…”

PENGARUH PEMBERIAN SERBUK BIJI LABU KUNING (Cucurbita moschata) TERHADAP PENURUNAN KOLESTEROL LDL PADA TIKUS WISTAR HIPERKOLESTEROLEMIA

“…Selain itu, beta karoten juga mampu mengikat kolesterol LDL akibat sifat beta karoten yang lipofilik. 27 Penurunan kolesterol LDL kelompok P2 lebih besar dibandingkan dengan kelompok P3 disebabkan karena asupan makan kelompok P3 lebih besar dibandingkan kelompok P2 yang memungkinkan total kalori kelompok P3 lebih besar dibandingkan kelompok P2. Total kalori merupakan jumlah kalori yang diperoleh dari asupan makan selama satu hari pada tikus.…”