Pengaruh Likopen terhadap Penurunan Aktivitas Mitogen-Activated Protein Kinase (MAPK) dan Ekspresi Endothelin-1 (ET-1) pada Kultur Huvecs yang Dipapar Leptin

Abstract

The effect of obesity on vascular function is mediated by hormon leptin. Leptin has been proved to increase oxidative stress in endothelial cell. The previous study has proven that leptin caused the endothelial dysfunction as a step of the atherogenesis. Lycopene, an antioxidant, is presumed having the ability to block the atherogenesis mechanism, which is stimulated a proinflamatory cytokine and adhesion molecules by MAPK and transcription factor ET-1. Therefore, the aim of this research was to prove and to determine whether lycopene could decrease the MAPK and ET-1 expression in Human Umbillical Vein Endothelial Cells (HUVECs) culture induced by 500 ng/ml leptin. In vitro study used primary culture of the HUVECs were devided in to 7 groups, there were (1) 0 ng/ml leptin and 0 ìM lycopene, (2) induced by 500 ng/ml leptin for 12 hours, (3) induced by leptin and lycopene with concentration 10; 25; 40; 55 and 75 ìM for 12 hours. Then the identification of MAPK was applied by using imunocytochemistry compared with ELISA procedure on cell endothel culture lysate and ET-1 expression was measured by using RT PCR. It was showed that lycopene 10-25 ìM decreased MAPK and ET-1 expression significantly in HUVECs culture induced by leptin 500 ng/ml. Leptin was increased ERK1/2 MAPK and ET-1 expression in HUVECs culture and can decrease by lycopene. Optimum dose of lycopene is 10-25 ìM.

PENGARUH PEMBERIAN UBI JALAR UNGU (Ipomoea batatas L. Poir) TERHADAP KADAR LEUKOSIT TOTAL TIKUS WISTAR JANTAN (Rattus norvegicus) YANG DIPAPAR ASAP ROKOK

“…NF-kB yang teraktivasi akan menginduksi transkripsi gen inflamasi diantaranya sitokin pro inflamasi TNF-α dan beberapa molekul adesi seperti ICAM-1 maupun VCAM. 65,66 Leukosit kemudian akan teraktivasi oleh sekresi dari sitokin ini. Selain itu, leptin juga diketahui mempunyai peran dalam lymphopoiesis dan myelopoiesis.…”