Pengaruh Desinfektan dan Lama Perendaman pada Sterilisasi Eksplan Daun Sukun (Artocarpus altilis (Parkinson ex. F.A Zorn) Fosberg)

Setiani, Nur Asni; Nurwinda, Fitri; Astriany, Dewi

doi:10.21776/ub.biotropika.2018.006.03.01

Cited by 11 publications

(12 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The addition of disinfectants containing sodium hypochlorite for surface sterilization of explants commonly used in many related studies (Felek et al 2015;Setiani et al 2018;Sulistiyo et al 2018;Zinabu et al 2018). Sodium hypochlorite is known to have antibacterial activity by damaging bacterial cell walls.…”

Section: Discussionmentioning

confidence: 99%

“…External location is the contamination that appears does not reach explants or by the edge of the culture medium. According to Setiani et al (2018), external contamination can come from environment, while internal contamination comes from contaminants that live inside the explants. Other ways to eliminate internal contamination is by giving fungicides and antibiotics into the medium (Kritzinger et al 1997).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The Development of In Vitro Culture Sterilization Method of Gametophyte Explant Lopholejeunea sp.

et al. 2021

View full text Add to dashboard Cite

In vitro cultures of leafy liverworts are still facing significant challenges due to high-level of explant contamination. The sterilization process can easily damage the structure of liverwort after exposure to the disinfectant. This study was to determine the concentration and time exposure of commercial bleach as a disinfectant to suppress contamination using the gametophyte culture of Lopholejeunea sp. The experiment consisted of control and six treatment combinations of commercial bleach with concentration 0.5, 0.75, and 1% (v/v), and exposure time (60 and 90 seconds). The type and location of contamination, the color of the explants after sterilization, and response after 30 days were observed. The results showed that the 0.75% bleach with 60 and 90 seconds exposure time had a lower contamination until the 7th day of culture. The most common type of contamination is bacteria and fungi that arise from the explant. Despite the contamination, it did not inhibit shoot formation. Further studies still needed to determine the type of fungicides and antibiotics with the most potent concentration and exposure time should be tested to obtain an axenic and viable culture of liverworts Lopholejeunea sp.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The Development of In Vitro Culture Sterilization Method of Gametophyte Explant Lopholejeunea sp.

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The old explants produced more oxidation than the younger explants [21]. The research results by Setiani et al [22] that sterilization of leaf explants Artocarpus altilis using 5.25% NaOCl for 10 minutes obtained browning explants reaching 90%. Meanwhile, Natasha & Restiani [23] reported that the 5% NaOCl concentration for 5 minutes in the sterilization of Javanese Ginseng leaf Talium paniculatum explants effectively suppressed browning.…”

Section: Percentage Of Browningmentioning

confidence: 99%

Effect of Explants Sterilization and Plant Growth Regulators on Embryo Culture of Kepel (Stelechocarpus burahol)

Handayani

Irsyadi

Alawiyah

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

View full text Add to dashboard Cite

Kepel (Stelechocarpus burahol) is an original Indonesian fruit plant. Kepel is currently hard to find and cultivate conventionally. In vitro culture is a method for kepel propagation. The in vitro culture requires an established protocol for explant sterilization to be successful. For the present, there is no appropriate kepel sterilization method that has been known. This study aimed to obtain a sterilization method for kepel embryo explants appropriate for embryo culture. This research was conducted in September 2018 - January 2019 at the In Vitro Culture Laboratory, Faculty of Agriculture, Universitas Muhammadiyah Yogyakarta. This study used an experimental method arranged in a single factor. Completely Randomized Design with the treatment of various immersion methods of kepel embryo explants divided into 8 levels. They are Sodium hypochlorite (NaOCl) (5% : 5', 5% : 10', 10% : 5', 10% : 10'), Hydrogen peroxide (H2O2) (2% : 10', 2% : 15', 3% : 10', 3% : 15') and plant growth regulators 2 ppm 2,4-D + 0,5 ppm BAP, 2 ppm NAA + 0,5 ppm BAP. The results showed that 5% NaOCl for 5 minutes treatment was the best method for sterilization with a percentage of viable explant 88.89%. Then, the percentage of browning 11.11% and 2 ppm 2,4-D + 0,5 ppm BAP was effective in the organogenesis of callus of kepel embryos.

show abstract

“…Kontaminasi yang disebabkan oleh bakteri akan menyebabkan eksplan tanaman menjadi basah karena adanya cairan berupa lendir sedangkan kontaminasi jamur eksplan tanaman akan lebih kering dan akan muncul hifa jamur pada tanaman yang terserang dan biasanya dapat dicirikan dengan garis-garis seperti benang yang berwarna putih sampai abu-abu (Asni et al, 2018).…”

Section: Hasil Dan Pembahasanunclassified

Optimasi teknik sterilisasi dan induksi tunas tanaman durian (Durio zibethinus Murr) ‘Kamajaya’ lokal Cimahi Secara in vitro

Rahmadi

Wicaksana²,

Nurhadi³

et al. 2020

kultivasi

View full text Add to dashboard Cite

Sari. Durian ‘Kamajaya’ merupakan salah satu jenis durian lokal yang keberadaanya hampir punah sehingga perlu dilakukan konservasi, salah satunya yaitu dengan perbanyakan menggunakan kultur jaringan. Permasalahan yang muncul dalam kultur jaringan durian ini salah satunya adalah metode sterilisasi. Penelitian ini bertujuan untuk memperoleh metode sterilisasi yang tepat untuk perbanyakan tanaman durian ‘Kamajaya’ secara in vitro. Penelitian ini dilaksanakan di Laboratorium Kultur Jaringan Teknologi Benih, Fakultas Pertanian, Universitas Padjadjaran, sejak bulan Mei sampai September tahun 2019. Eksplan yang digunakan adalah tunas muda dari tanaman durian ‘Kamajaya’ yang berasal dari Cimahi. Sterilisasi untuk inisiasi dengan teknik kultur jaringan menggunakan kombinasi air mengalir, detergen, fungisida, bakterisida, alkohol 70%, antiseptik PCMX 10%, surfaktan polysorbate 3 tetes per liter, antiseptik povidone-iodine, clorox (10% dan 20%) dan HgCl2 0,1%. Hasil penelitian menunjukan bahwa Sterilisasi tunas muda durian ‘Kamajaya’ dengan menggunakan air mengalir, perendaman dalam detergen selama 10 menit, perendaman dalam fungisida selama 10 menit, perendaman dalam clorox 20% + surfaktan polysorbate 3 tetes per L selama 20 menit, perendaman dalam clorox 10% selama 10 menit, serta perendaman dalam HgCl2 0,1% selama 1 menit memiliki presentase kontaminasi terendah yaitu 20 % dan presentasi hidup tertinggi yaitu 80 %.Kata kunci: Durian ‘Kamajaya’ ∙ Kultur jaringan ∙ Sterilisasi Abstract. Durian 'Kamajaya' is a type of local durian that is almost extinct. Tissue culture is one of the methods that can be used as a plant conservation. However, many problems arise in Durian tissue culture, one of which is the sterilization method. The objective of this study was to obtain an appropriate in vitro sterilization method for the multiplication of Durian 'Kamajaya'. The research was conducted at the Laboratory of Tissue Culture, Faculty of Agriculture, Padjadjaran University, from May to September 2019. The durian buds were used as plant explant sterilized for initiation with tissue culture techniques using different sterilization methods as following running water, detergent, fungicides , bactericide, alcohol 70% , PCMX antiseptic 10%, polysorbate surfactant 3 drops per L, clorox (10% and 20%), and HgCl2 0.1%. The results showed that the sterilization of Durian 'Kamajaya' using water flow + soaking in detergent for 10 minutes + fungicide for 10 minutes + Clorox 20% and polysorbate surfactant 3 drops per L for 20 minutes + Clorox 10% for 10 minutes + HgCl2 0.1% for 1 minute gave the lowest percentage of contamination (20%) and the highest life presentation(80%).Keywords: Durian 'Kamajaya' ∙ Tissue culture ∙ Sterilization

show abstract

Pengaruh Desinfektan dan Lama Perendaman pada Sterilisasi Eksplan Daun Sukun (Artocarpus altilis (Parkinson ex. F.A Zorn) Fosberg)

Cited by 11 publications

References 0 publications

The Development of In Vitro Culture Sterilization Method of Gametophyte Explant Lopholejeunea sp.

The Development of In Vitro Culture Sterilization Method of Gametophyte Explant Lopholejeunea sp.

Effect of Explants Sterilization and Plant Growth Regulators on Embryo Culture of Kepel (Stelechocarpus burahol)

Optimasi teknik sterilisasi dan induksi tunas tanaman durian (Durio zibethinus Murr) ‘Kamajaya’ lokal Cimahi Secara in vitro

Contact Info

Product

Resources

About