Effect of Explants Sterilization and Plant Growth Regulators on Embryo Culture of Kepel (Stelechocarpus burahol)

Handayani, Eka; Irsyadi, Muhammad Burhanuddin; Alawiyah, R L M N; Aris, Irfan

doi:10.1088/1755-1315/985/1/012016

Cited by 7 publications

(7 citation statements)

References 15 publications

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“…Amaranthus hybridus seeds were received from Kerala Agricultural University, Mannuthy, India. Seeds were surface sterilized with 5% Sodium hypochlorite for 5 min and rinsed thoroughly with sterile DI water [ 33 ]. Next, 10 g of seeds were soaked for 8 h in 50 mL of 0.06, 0.12, and 0.24 µm CuO and ZnO nanoparticles solution, with sterile DI water as a negative control.…”

Section: Methodsmentioning

confidence: 99%

Biogenic CuO and ZnO Nanoparticles as Nanofertilizers for Sustainable Growth of Amaranthus hybridus

Francis

Sood²,

Gokhale³

2022

Plants

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The biogenic synthesis of CuO and ZnO nanoparticles (NPs) was carried out by Stenotrophomonas maltophilia. The shape, size, and chemical identity of the CuO and ZnO NPs were determined using FTIR, XRD, SEM, EDX, and TEM analysis. The study aimed to investigate the effects of the CuO and ZnO NPs on Amaranthus hybridus seed germination and plant growth. Two different fertilizer application modes (hydroponics and foliar) were studied with varying concentrations of CuO (0.06 µM, 0.12 µM) and ZnO (0.12 µM, 0.24 µM) nanoparticles with water control and Hoagland’s media control. The hydroponic system of fertilizer application demonstrated better efficiency in terms of plant growth as compared to the foliar application. The agronomic traits, SPAD value, total reducing sugars, antioxidant activity, amount of copper, and zinc ions in root and shoot were analyzed for all experimental plants and found better with the nanoparticle application. The highlight of the study is the application of extremely low concentrations of CuO and ZnO nanoparticles, almost 70% lower than the copper and zinc salts in the Hoagland’s medium for improved plant growth. The use of lower concentrations of nanoparticles can prevent their accumulation in the environment and also lower the production cost. The high antioxidant concentration exhibited by the plants treated with CuO and ZnO nanoparticles ensures the enhanced plant’s resistance to infections and pests while promoting plant growth.

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Section: Methodsmentioning

confidence: 99%

Biogenic CuO and ZnO Nanoparticles as Nanofertilizers for Sustainable Growth of Amaranthus hybridus

Francis

Sood²,

Gokhale³

2022

Plants

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“…Media yang sesuai untuk pertumbuhan kalus yang umum digunakan untuk tanaman budidaya adalah media MS (Murashige & Skoog) dengan tambahan zat pengatur tumbuh. Zat pengatur tumbuh sintetik perlu ditambahkan karena zat pengatur umbuh yang terbentuk secara alami seringkali tidak mencukupi pertumbuhan jaringan eksplan (Handayani et al, 2022). Zat pengatur tumbuh yang umum digunakan untuk induksi kalus adalah auksin dan sitokinin.…”

Section: Perlakuanunclassified

Studi pendahuluan regenerasi eksplan teh sebagai upaya percepatan penyediaan bibit unggul secara in vitro

Anjarsari¹,

Suminar²,

Murgayanti³

2022

Kultivasi

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Studi pendahuluan regenerasi eksplan teh sebagai upaya percepatan penyediaan bibit unggul secara in vitroSari. Pembiakan generatif tanaman teh yang dilakukan dengan biji, sementara secara vegetatif dengan setek tunas yang mempunyai kelemahan, antara lain jumlah bibit yang dihasilkan terbatas, perlu waktu lama untuk menyeleksi pohon induk, dan bibit yang dihasilkan kurang optimal dalam penyerapan air dan unsur hara karena perakarannya dangkal sehingga kurang toleran terhadap kekeringan. Kultur jaringan menjadi salah satu alternatif dalam perbanyakan teh memperoleh klon teh unggul, seperti klon Seri Gambung 1-11, dalam jumlah banyak dan sifatnya yang seragam. Penelitian pendahuluan ini dilaksanakan di Laboratorium Kultur Jaringan Departemen Budidaya Pertanian Fakultas Pertanian UNPAD, mulai bulan Juni hingga Desember 2021. Penelitian terdiri dari dua tahap, yaitu (i) metode deskriptif untuk menginduksi eksplan organ teh (pucuk + ruas batang serta daun), dan (ii) metode eksperimen dengan menguji eksplan yang ditumbuhkan pada berbagai media. Metode percobaan yang digunakan untuk penelitian ke-2 adalah rancangan acak lengkap (RAL) dengan 6 kombinasi perlakuan Benzyl Amino Purine (BAP) (0 dan 0,5 ppm) dan 2,4-D (0,05; 0,1; dan 0,2 ppm). Hasil pengamatan pendahuluan menunjukkan masih banyak eksplan yang mengalami kematian, namun beberapa eksplan potongan daun menunjukkan respon yang baik dengan mampu membentuk kalus berwarna hijau. Kombinasi 0,5 ppm BAP + 0,05 ppm 2,4-D memberikan pengaruh lebih baik terhadap bobot kalus, sedangkan kombinasi 0,5 ppm BAP + 0,2 ppm 2,4D memberikan pengaruh baik terhadap diameter kalus. Kata kunci : Eksplan teh • In vitro • Klon unggul Preliminary study of tea explant regeneration as an effort to accelerate the supply of superior seeds by in vitroAbstract. Generative propagation of tea plants is done by seed, while the vegetative method is by shoot cuttings which have weaknesses, including the limited number of seedlings produced, it takes a long time to select the mother tree, and the resulting seedlings are not optimal in absorbing water and nutrients because their roots are shallow making it less tolerant of drought. Tissue culture is an alternative in tea propagation to obtain superior tea clones, such as the Gambung Series 1-11 clones, in large quantities and with uniform characteristics. This preliminary research was carried out at the Tissue Culture Laboratory of the Department of Agronomy, Faculty of Agriculture, UNPAD, from June to December 2021. The research consisted of two stages, namely (i) a descriptive method to induce tea organ explants (shoots + stem segments and leaves), and (ii) an experimental method by testing explants grown on various media. The experimental method used for the second study was a completely randomized design (CRD) with 6 treatment combinations of Benzyl Amino Purine (BAP) (0 and 0.5 ppm) and 2.4 D (0.05; 0.1; and 0 .2 ppm). Preliminary observations showed that there were still many death explants observed, however, some explants in form of cut leaves ...

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“…Ex situ conservation attempts using in vitro culture can be used to effectively propagate this plant. Several studies related to in vitro culture of Kepel plants have been conducted with several focuses, including optimization of sterilization methods for leaf explants (Habibah et al, 2013), nodes (Hutabarat et al, 2022) and endosperm (Handayani et al, 2022), optimization of PGR that supports callus growth of Kepel seed and fruit mesocarp explants (Habibah et al, 2016(Habibah et al, , 2017(Habibah et al, , 2019, and nodal explant (Sekar et al, 2023), and optimization of flavonoid production from callus and cell suspension cultures of Kepel plants (Habibah et al, 2016(Habibah et al, , 2019. However, in vitro propagation of Kepel plants still faces obstacles, including the browning of explants.…”

Section: Introductionmentioning

confidence: 99%

Browning Prevention Method in Kepel (Stelechocarpus burahol (Blume) Hook.f. & Thomson) in Vitro Culture

Sibarani,

Restiani,

Prasetyaningsih

2024

JPBN

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Browning of explants is a common problem in Kepel (Stelechocarpus burahol (Blume) Hook.f. & Thomson) in vitro culture, resulting in low explant in vitro regeneration of Kepel. Using nodal explant, the effect of explant immersion in ascorbic acid, the addition of ascorbic acid to the media, and dark and light incubation conditions were investigated. The browning prevention method was selected based on the delayed browning appearance time, the lowest broning intensity, and the highest percentage of callus. This present study used a completely randomized design with treatment variations: immersion explant in ascorbic acid 100 mg/L, addition of ascorbic acid 100 and 200 mg/L to the media, addition of ascorbic acid 100 and 200 mg/L and activated charcoal 1 g/L to the media, and incubation in dark and light conditions for 28 days. The results showed that the combination of the addition of ascorbic acid 200 mg/L to MS media and incubation in dark conditions were effective browning prevention methods in inhibiting browning appearance time (5 DAP), significantly reducing browning intensity (0.3), and increasing callus growth (100%) of kepel node explants during 28 days of culture. The results of this study are useful in establishing protocols for in vitro culture of Kepel plants, especially at the initiation stage, and can be expected to support the successful conservation of Kepel plants through in vitro propagation. Keywords: Ascorbic acid, Activated Charcoal, Browning, In Vitro Culture, Stelechocarpus burahol

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Effect of Explants Sterilization and Plant Growth Regulators on Embryo Culture of Kepel (Stelechocarpus burahol)

Cited by 7 publications

References 15 publications

Biogenic CuO and ZnO Nanoparticles as Nanofertilizers for Sustainable Growth of Amaranthus hybridus

Biogenic CuO and ZnO Nanoparticles as Nanofertilizers for Sustainable Growth of Amaranthus hybridus

Studi pendahuluan regenerasi eksplan teh sebagai upaya percepatan penyediaan bibit unggul secara in vitro

Browning Prevention Method in Kepel (Stelechocarpus burahol (Blume) Hook.f. & Thomson) in Vitro Culture

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