PCR- Based Test for the Early Warning of Both Cylindrospermopsin and Saxitoxin in Iraqi Freshwater

Abed, Ibrahim J.

doi:10.22401/jnus.18.3.15

Cited by 4 publications

(1 citation statement)

References 5 publications

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“…Harmful blooms of cyanobacteria represent a threat to human and environmental health and also resulting in unfavorable economic impacts. Exposure to secondary toxic metabolites (cyanotoxins) formed by cyanobacteria during skin contact and consuming of water leads to human and animal illness and in some cases lead to death [3]. However, cases of human intoxications have been reported following exposure to Microcystis and Plantothrix blooms [4].…”

Section: Introductionmentioning

confidence: 99%

PCR –Based Detection of Microcystin and Nodularin in both Freshwater and Bloom in Tigris River

Abed

Abdulhasan

Zhang

et al. 2018

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Cyanobacterial blooms have a range of social, environmental and economic impacts due to their content of secondary metabolites involving toxins. Microcystins (MCs) is one of the cyanotoxins found in freshwater ecosystems. This study was aimed to adopt a rapid technique for determining the microcystin in blooms and freshwater by the detection of phycocyanin operon of cyanobacteria using PCβF and PCαR primers as well as mcyE gene encoding to microcystin/ nodularin using HEP primers. The molecular results showed that phycocyanin operon and mcyE gene were disclosed in the freshwater and bloom of samples at the studied sites. In conclusion, The PCR assay used in this study was helpful and rapid, in particular when the target organism concentration in the freshwater sample is very low.

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Section: Introductionmentioning

confidence: 99%

PCR –Based Detection of Microcystin and Nodularin in both Freshwater and Bloom in Tigris River

Abed

Abdulhasan

Zhang

et al. 2018

ANJS

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Molecular detection of some toxogenic cyanobacteria in Tigris River in Baghdad–Iraq

2021

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Cyanobacteria and their emissions are becoming more widely reported around the world, posing a serious threat to both the environment and human health. Several orders of cyanobacteria have been identi ed to make cyanotoxin, the most common algal toxin. The aim of this research was to develop a method for detecting cylindrosprmopsin and saxitoxin biosynthesis genes in rivers .In November, December 2019 and January2020. Cyanobacteria were isolated from Tigris River freshwater and detected using a compound microscope as well as traditional PCR .All cyanobacteria isolates contained phycocyanin gene fragment.Five isolates of cyanobacteria in these study was successfully ampli ed a phycocyanin gene (Microcystis osaquae, Microcystis sp, anabaena circinalis ,nostoc commune and westiellopsis proli ca) and all isolates successfully ampli ed aoaC gene to detecting the cylidrospemopsin and the saxitoxin. Our ndings show that a PCR assay can be used to detect cylidrospemopsin and saxitoxin-producing cyanobacteria in river water, which is useful for stations that prepare drinking water for the public.

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Quick detection method for paralytic shellfish toxins (PSTs) monitoring in freshwater - A review

Persson

2021

Chemosphere

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PCR- Based Test for the Early Warning of Both Cylindrospermopsin and Saxitoxin in Iraqi Freshwater

Cited by 4 publications

References 5 publications

PCR –Based Detection of Microcystin and Nodularin in both Freshwater and Bloom in Tigris River

PCR –Based Detection of Microcystin and Nodularin in both Freshwater and Bloom in Tigris River

Molecular detection of some toxogenic cyanobacteria in Tigris River in Baghdad–Iraq

Quick detection method for paralytic shellfish toxins (PSTs) monitoring in freshwater - A review

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