This study was designed to evaluate the antimicrobial effect of Cymbopogon citratus and Mentha spicata essential oils, separately and mixed, against the microorganisms in yogurt, as well as study the possibility of these essential oils (EO) as natural preservatives and flavors additives/enhancers in yogurt product. Yogurt samples were treated with lemongrass and spearmint EOin different concentrations (250, 500, 1000 ppm: 6250µg/50 ml yogurt, 12500 µg/50 ml yogurt and 25000 µg/50 ml yogurt respectively). The control and treated samples were preserved both at room (25°C) and refrigerator (5°C) temperatures. In control, the contamination was observed through 2 weeks at 25°C and for about one month at 5°C. The samples treated with lemongrass and spearmint EOs (500pmm), the contamination showed up late, after 45 and 30 days at 25°C respectively. While at 5°C, the contamination appeared after 90 and 60 days respectively. The effect of lemongrass and spearmint EO, separately or mixed (synergistic effect), on the growth of fungi that was isolated from spoiled samples, was studied in different concentrations (125, 250, 500 ppm). Microbilogical examination was done in the control and treated yogurt samples. There was a significant difference (P≤ 0.05) between microbial spoilage (coliform, yeast and fungi) count during different periods of incubation which decreased in the samples treated with essential oils as compared with the control. The results of the percentage of growth inhibition revealed that lemongrass EO, around 80-100%, is the best in inhibiting the molds and yeasts causing yogurt damage as compared to the use of spearmint EO which was 27-60%, and the synergistic effect of about 35-39%. The results of the toxicity assay of the maximum effect of EO in vivo proved their validity for consumption when added both as preservative and flavor. The concentrations used for the dosage ranged from 250 ppm to 5000 ppm.
This study was aimed to investigate the antiproliferative effect of lemongrass essential oil (LEO) from Cymbopogon citratus on A549 cell line and their effect on the expression of p53 gene in lung cancer cells. The LEO was extracted and anlaysed using GC-MS analysis, the cytotoxicity of LEO against lung cancer cells was examined using MMT assay and finally the expression of p53 was determined via qRT-PCR. The chromatogram of GC-MS exhibited 14 compounds in ELO, the major one is citral (56.4%). The cytotoxicity of LEO was dose dependent which proliferation of the lung cells were significantly inhibited with increased concentration of essential oil (P≤ 0.01) as well as the IC50 was 29.13 ppm. Furthermore, it showed that cell treated with IC50 of LEO had a significant higher gene expression (P≤ 0.05) in the fold of p53 expression than untreated cells with change fold of 4.47±0.7. In conclusion, C. citratus essential oil have been found to possess antiproliferative activity against lung cancer cells and upregulation of p53 gene which can be considered a promising treatment for lung cancer patients.
Aeromonas hydrophila is widely distributed throughout the world and causes diseases to animals and human exposed to contaminated environments such as water and soil. This study aimed to compare between isolates of A. hydrophila collected from clinical and environmental samples, through investigating the phenotype of some virulence factors in vitro, including hemolysin, protease, lipase, nuclease and biofilm formation ability. Also, the antimicrobial susceptibility for different antibiotics was determined using disc diffusion method. For genotypic identification of isolates and phylogenetic tree construction, 16S rDNA target gene was amplified and sequenced. The phenotypic results showed some differences between the isolates (clinical and environmental). All isolates were resistance to clindamycin, amoxicillin and erythromycin while susceptible to gentamicin, amikacin and vancomycin. Sequences of 16S rDNA confirmed the identification of the studied bacteria as A. hydrophila with 99-100% , and identity and phylogenetic tree by neighbor-joining clearly separated the isolates in a branching pattern which displayed similarity to the GenBank isolates obtained from Asian regions. The clinical isolates showed less polymorphism than the environmental isolates.
Urinary tract infections (UTI) are among the most common infections in humans worldwide
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