Padronização do diagnóstico de Blastocystis hominis por diferentes técnicas de coloração

Eymael, Dayane; Schuh, Graziela Maria; Tavares, Rejane Giacomelli

doi:10.1590/s0037-86822010000300019

Cited by 11 publications

(7 citation statements)

References 18 publications

(34 reference statements)

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“…16 The absence of this parasite in our study may be, in part, due to the relative unfamiliarity with this protozoan and the low sensitivity of our diagnostic methods with regard to this intestinal protozoa. 17 In our study, poverty was the main predictor for a parasitic infection in these preschool children. This is consistent with other reports.…”

Section: Discussionmentioning

confidence: 47%

Risk factors for intestinal parasitic infections in preschoolers in a low socio-economic area, Diamantina, Brazil

Nobre

Silva

Macedo

et al. 2013

Pathogens and Global Health

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Objective: To verify the prevalence of intestinal parasitic infections among preschoolers and to identify the associated risk factors. Methods: The study is a cross-sectional study nested in a cohort of children who were born and resident in Diamantina, Minas Gerais, Brazil. At the time of the study, all children were aged 60 months 6 five months. They were recruited after written informed consent was obtained from parents or guardians. The study was carried out between July 2009 and July 2010. In total 214 children provided a stool sample for examination on intestinal parasitic infections. Information on potential risk factors for parasitosis was obtained from parents and guardians of the children by a questionnaire. Logistic regression was used for analysis. Results: Intestinal parasitic infections were found in 27.5% (n559) of children. The boys' infection prevalence (26.1%, n536) was slightly lower than the infection prevalence of the girls (30.3%, n523), but not statistically different (p50.51). Fourteen children, (23.7%) were infected with two or more parasite species and forty-five (76.3%) with single parasites. A low per capita income of family was strongly associated with an increased risk for an infection (OR52.89; P50.003). Preschoolers whose mothers did not work outside home had a significantly lower risk for infection (OR50.41; p50.01).

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Section: Discussionmentioning

confidence: 47%

Risk factors for intestinal parasitic infections in preschoolers in a low socio-economic area, Diamantina, Brazil

Nobre

Silva

Macedo

et al. 2013

Pathogens and Global Health

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“…Santos et al [56] Malheiros et al [60] Batista et al [59] Visser et al [61] Eymael et al [62] Aguiar et al [67] Souza et al [70] Quadros et al [73] Heterogeneity between groups: p = 0.000…”

Section: Goiás Santa Catarinamentioning

confidence: 99%

Prevalence of Blastocystis sp. infection in several hosts in Brazil: a systematic review and meta-analysis

et al. 2020

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Background: Blastocystis sp. affects a wide variety of animals and is the most common protozoan in human fecal samples with potential pandemic distribution. In the present study, a systematic review and meta-analysis were conducted to determine the prevalence and distribution of Blastocystis sp. in different classes of hosts in Brazil. Methods: Studies that analyzed hosts of various classes, including humans, domestic animals, wild animals or captive animals, were considered. The pooled prevalence of Blastocystis sp. infection was estimated by random effects models. Results: For humans, similar prevalence rates were found for males (31.0%, 95% CI: 17.0-45.0%; weight 10%) and females (28.0%, 95% CI: 16.0-41.0%; weight 10%); the state of Mato Grosso do Sul showed the highest prevalence, with 41.0% positivity (95% CI: 36.0-46.0%; weight 2.9%). The prevalence among immunocompromised patients was 5.0% (95% CI: 3.0-7.0%; weight 10%), and the most common cause of immunosuppression was hemodialysis, with 23.0% (95% CI: 17.0-29.0%; weight 12.4%). Among classifications according to interaction with humans, wild and domestic animals presented values of 19.0% (95% CI: 7.0-31.0%; weight 42.6%) and 17.0% (95% CI: 13.0-21.0%; weight 29.6%), respectively. Among these animals, mammals (39.0%, 95% CI: 21.0-56.0%; weight 47.3%) and birds (18.0%, 95% CI: 10.0-27.0%; weight 39.3%) exhibited the highest prevalence. Phylogenetic analysis of Blastocystis sp. revealed greater genetic diversity for clades of subtypes (STs) ST1, ST2 and ST3. Conclusions: The overall prevalence of Blastocystis sp. in the Brazilian human population was 24%, which reflects the reality in the South, Southeast and Midwest regions, where prevalence rates of up to 40% were found. Among animals, mammals and birds exhibited the highest prevalence.

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“…Conversely, other studies, including this one, have shown that CS is a more effective technique for identification of Blastocystis spp. in faecal samples (12). The better performance of CS in Blastocystis spp.…”

Section: Discussionmentioning

confidence: 98%

Concordance between the zinc sulphate flotation and centrifugal sedimentation methods for the diagnosis of intestinal parasites

et al. 2016

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Introduction:The diagnosis of intestinal parasitic infections depends on the parasite load, the specific gravity density of the parasite eggs, oocysts or cysts, and the density and viscosity of flotation or sedimentation medium where faeces are processed. Objective: To evaluate the concordance between zinc sulphate flotation and centrifugal sedimentation in the recovery of parasites in faecal samples of children. Materials and methods: Faecal samples of 330 children from day care centers were evaluated by zinc sulphate flotation and centrifugal sedimentation techniques. The frequencies of detection of parasites by each method were determined and the agreement between the diagnostic techniques was evaluated using the kappa index, with 95% confidence intervals. Results: The faecal flotation in zinc sulphate diagnosed significantly more cases of Trichuris trichiura infection when compared to centrifugal sedimentation (39/330; 11.8% vs. 13/330; 3.9%, p<0.001), with low diagnostic concordance between methods (kappa=0.264; 95% CI: 0.102-0.427). Moreover, all positive samples for Enterobius vermicularis eggs (n=5) and Strongyloides stercoralis larvae (n=3) were diagnosed only by zinc sulphate. No statistical differences were observed between methods for protozoa identification. Conclusions:The results showed that centrifugal flotation in zinc sulphate solution was significantly more likely to detect light helminths eggs such as those of T. trichiura and E. vermicularis in faeces than the centrifugal sedimentation process.

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Padronização do diagnóstico de Blastocystis hominis por diferentes técnicas de coloração

Cited by 11 publications

References 18 publications

Risk factors for intestinal parasitic infections in preschoolers in a low socio-economic area, Diamantina, Brazil

Risk factors for intestinal parasitic infections in preschoolers in a low socio-economic area, Diamantina, Brazil

Prevalence of Blastocystis sp. infection in several hosts in Brazil: a systematic review and meta-analysis

Concordance between the zinc sulphate flotation and centrifugal sedimentation methods for the diagnosis of intestinal parasites

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