Concordance between the zinc sulphate flotation and centrifugal sedimentation methods for the diagnosis of intestinal parasites

Inês, Elizabete de Jesus; Pacheco, Flávia Thamiris Figueiredo; Pinto, Milena Carneiro; Mendes, Paulo; Costa-Ribeiro, Hugo; Soares, Neci Matos; Teixeira, Márcia Cristina Aquino

doi:10.7705/biomedica.v36i4.2799

Cited by 8 publications

(7 citation statements)

References 17 publications

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“…Comparison of several microscopy techniques showed that trichrome stain was able to detect most parasites, whereas zinc sulphate flotation technique was slightly better than the wet mount, and it was not suitable for Blastocystis spp. as the chemical seems to induce damage of the parasite´s membranes [ 46 , 47 ]. The combination of microscopy techniques used in this study presumptively detected 8% of pathogenic protozoa and no detection of Cryptosporidium spp.…”

Section: Discussionmentioning

confidence: 99%

Molecular diagnosis of intestinal protozoa in young adults and their pets in Colombia, South America

Potes-Morales

Crespo-Ortiz

2023

PLoS ONE

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Intestinal parasitic infections have been considered a relevant public health problem due to the increased incidence worldwide. In developing countries, diarrhea and gastrointestinal symptoms cause impaired work capacity in adults and delayed rate growth in children. Enteric infections of unknown etiology can often lead to misdiagnosis, increased transmission, and morbidity. The aim of this study was to determine the prevalence of intestinal parasites in a young adult population and their pets. Stool samples from 139 university students and 44 companion animals were subjected to microscopy diagnosis using wet mounts, concentration by zinc sulphate flotation and staining techniques (Kinyoun and trichrome stain). Molecular diagnosis of protozoa was also performed by conventional PCR. The mean age was 24 years, 54% individuals were female, 46% were men, and 66% had at least one pet. The overall prevalence for at least one parasite was 74.8% and the rate of polyparasitism was 37.5%. Eighty-three patients (59.7%) were positive for Blastocystis spp., followed by Cryptosporidium spp. 24.5%, Endolimax nana 13.6%, Entamoeba dispar/E. moshkovskii 7.8% and Giardia intestinalis 1.4%. Molecular diagnosis substantially improved Cryptosporidium spp. and Blastocystis spp. detection and allowed to distinguish E. histolytica from commensals in the Entamoeba complex. Student’s pets were also examined for parasitism. Samples from 27 dogs, 15 cats, one rabbit and one hen were analyzed, and parasites were detected in 30 (68.2%) as follows: Cryptosporidium spp. (24) Giardia spp. (4), hookworm (3), Endolimax nana (2) and Toxoplasma gondii (1). Overall, university students showed high prevalence of parasitism and polyparasitism suggesting exposure to parasite infected animals and contaminated environments. Cryptosporidium spp. was the predominant pathogen in human and domestic animals, and it was only detected by PCR, pointing out the need for sensitive tests in diagnosis and surveillance. Control strategies to prevent the effects of parasitic infections in young population should consider pets as reservoirs and transmission source.

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Section: Discussionmentioning

confidence: 99%

Molecular diagnosis of intestinal protozoa in young adults and their pets in Colombia, South America

Potes-Morales

Crespo-Ortiz

2023

PLoS ONE

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“…Adhesive tape testing for EV ova may aid diagnosis. Although not routine practice in Australasia, a Brazilian study identified EV ova in stool samples of five children using centrifugal flotation in zinc sulphate solution . Increased awareness of EV may lead to the medical treatment of appendiceal colic without the trauma and cost of surgery.…”

Section: Discussionmentioning

confidence: 99%

Needles in the haystack: Enterobius vermicularis in the appendix

Ball

Newsom

2019

J Paediatrics Child Health

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“…The sample analysis was conducted via a direct examination with a physiological saline solution and a 3% iodine solution. Additionally, the fecal concentration technique was performed with a zinc sulfate concentration of 33.0% for the concentration of eggs (2 g of feces were homogenized in 10 mL of water, filtered through gauze, and centrifuged in a 15 mL tube for 2 minutes at 400× g , and the sediment was resuspended in a zinc sulfate solution) [ 13 , 14 ].…”

Section: Methodsmentioning

confidence: 99%

Comparative study of the intestinal parasitism profiles between communities across the 5 municipalities of the Barranquilla metropolitan area, Colombia

Sarmiento-Rubiano¹,

Filott

Gómez

et al. 2021

Osong Public Health Res Perspect

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Objectives: The aim of this study was to compare the rate of parasitism and intestinal parasitism profiles of children who live in relatively proximal communities across the 5 municipalities of the Barranquilla metropolitan area, Colombia.Methods: In total, 986 fecal samples from children aged 1 to 10 were analyzed using a direct method (physiological saline and lugol) and the zinc sulfate flotation technique. A comparative analysis of the parasitism profiles between sampling locations (7 schools and 3 health centers providing growth and development services) was conducted using principal component analysis (PCA). The presence of Taenia solium antibodies was evaluated by the enzyme-linked immunosorbent assay method in 269 serum samples from the same group of children.Results: The overall prevalence of intestinal parasitism was 57.6% (range, 42.1%–77.6%) across the sampling areas. The prevalence of helminthiasis was between 4.1% and 23.7%, and that of intestinal protozoa was between 38.2% and 73.5%. PCA showed that the parasite profiles of each sampling location shared no common characteristics. A total of 3.0% of the serum samples were positive for T. solium antibodies.Conclusion: The intestinal parasitism profiles between relatively proximal sampling locations with similar geographic conditions were vastly different, indicating the need to study each small ecological niche on a localized scale to develop more cost-effective interventions for controlling intestinal parasitism.

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Concordance between the zinc sulphate flotation and centrifugal sedimentation methods for the diagnosis of intestinal parasites

Cited by 8 publications

References 17 publications

Molecular diagnosis of intestinal protozoa in young adults and their pets in Colombia, South America

Molecular diagnosis of intestinal protozoa in young adults and their pets in Colombia, South America

Needles in the haystack: Enterobius vermicularis in the appendix

Comparative study of the intestinal parasitism profiles between communities across the 5 municipalities of the Barranquilla metropolitan area, Colombia

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