PACAP stimulation of maturational gonadotropin secretion in goldfish involves extracellular signal-regulated kinase, but not nitric oxide or guanylate cyclase, signaling

Chang, John P.; Sawisky, Grant R.; Mitchell, Gabriel; Uretsky, Aubrey D.; Kwong, Patrick; Grey, Caleb L.; Meints, Amanda N.; Booth, Morgan

doi:10.1016/j.ygcen.2009.06.011

Cited by 4 publications

(4 citation statements)

References 66 publications

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“…Final concentrations were attained by diluting stock solutions in testing medium [medium 199 (M199) with Hanks salts (Gibco, Grand Island, NY, USA), 25 m m Hepes, 26.2 m m NaHCO 3 , 100 000 U penicillin/l and 100 mg streptomycin/l, pH adjusted to 7.18 with 10 N NaOH]. Working concentrations of pharmacological agents used are outlined in Table 1 and have been shown to be either maximally stimulatory [sGnRH and cGnRH‐II (40)] or effective [7‐Ni, AGH, and 1400W (33,35,41); PTIO, SNP and rutin hydrate (35,41); ODQ (42,43); dbcGMP (34)] in the goldfish pituitary cell system, or effective in other systems [KT5823 (44,45)].…”

Section: Methodsmentioning

confidence: 99%

“…First, immunoreactivity for inducible (i)NOS and nNOS has been detected in goldfish pituitary gonadotrophs (33). Second, the cell permeant cGMP agonist dibutyryl (db)cGMP elevates LH release from goldfish pituitary cells in static primary cell culture (34). On the other hand, NO/cGMP signalling in part mediates the growth hormone (GH) release responses to the two endogenous goldfish GnRHs, salmon (s)GnRH (Type 3 GnRH) and chicken (c)GnRH‐II (Type 2 GnRH) in goldfish pituitary cells (33,35).…”

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confidence: 99%

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Nitric Oxide and Guanylate Cyclase Signalling are Differentially Involved in Gonadotrophin (LH) Release Responses to Two Endogenous GnRHs from Goldfish Pituitary Cells

Meints

Pemberton

Chang

2012

J Neuroendocrinology

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Nitric oxide synthase (NOS) immunoreactivity is present in goldfish gonadotrophs. The present study investigated whether two native goldfish gonadotrophin-releasing hormones (GnRHs), salmon (s)GnRH and chicken (c)GnRH-II, use NOS/nitric oxide (NO) and soluble guanylate cyclase (sGC)/cyclic (c)GMP/protein kinase G (PKG) signalling to stimulate maturational gonadotrophin [teleost gonadotrophin-II, luteinising hormone (LH)] release. In cell column perifusion experiments with dispersed goldfish pituitary cells, the application of three NOS inhibitors (aminoguanidine hemisulphate, 1400W and 7-nitroindazole) and two NO scavengers [2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (PTIO) and rutin hydrate] reduced sGnRH-elicited, but not cGnRH-II-induced, LH increases. The NO donor sodium nitroprusside (SNP) increased NO production in goldfish pituitary cells in static incubation. SNP-stimulated LH release in column perifusion was attenuated by PTIO and the sGC inhibitor 1H-(1,2,4)oxadiazolo[4,3-a]quinoxalin-1-oneon (ODQ), and additive to responses elicited by cGnRH-II, but not sGnRH. ODQ and the PKG inhibitor KT5823 decreased sGnRH- and cGnRH-II-stimulated LH release. Similarly, the LH response to dibutyryl cGMP was reduced by KT5823. These results indicate that, although only sGnRH uses the NOS/NO pathway to stimulate LH release, both GnRHs utilise sGC/PKG to increase LH secretion.

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Nitric Oxide and Guanylate Cyclase Signalling are Differentially Involved in Gonadotrophin (LH) Release Responses to Two Endogenous GnRHs from Goldfish Pituitary Cells

Meints

Pemberton

Chang

2012

J Neuroendocrinology

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“…Among the parameters that can be changed to stimulate reproduction, photoperiod has been cited in several species [6][7][8]. Circadian rhythms determine the alterations in the hypothalamic-pituitary-gonadal axis, leading to release of gonadotropin-releasing hormone (GnRH) [8].…”

Section: Influence Of Photoperiod Over Morphometric and Hematologicalmentioning

confidence: 99%

“…Dopamine is known as the inhibitory hypothalamic-pituitary-gonadal axis in fish. Furthermore, melatonin have the ability to inhibit the hypothalamic-pituitary-gonadal axis via dopaminergic receptors [7].…”

Section: Influence Of Photoperiod Over Morphometric and Hematologicalmentioning

confidence: 99%

Influence of Photoperiod over Morphometric and Hematological Parameters of Juvenile Piracanjubas (Brycon orbygnianus)

Machado¹,

Andrade²,

Andrade³

et al. 2016

JAST-B

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The aim of this study was to evaluate morphometric and hematological parameters of juveniles Piracanjubas treated with two distinct photoperiod. Twenty nine fish with 100 g, in a factorial design were performed in 18 h light:6 h dark (18L:6D) and 6L:18D two different photoperiod, and morphometric and hematological parameters were evaluated at 45 d and 90 d after photoperiod treatment. Benzocaine 1% was used for euthanasia. Fish were weighed and measured, so as visceral fat, liver and gonads. Hematocrit, total count of white cells, lymphocytes and neutrophils were counted. Results showed that there was no significant relationship between bodyweight and standard length. Significant interaction (P = 0.00018) had been found between the fat index (FI) and gonado-somatic index (GSI) and the photoperiod and time. At 90 d, the fish kept in 18L:6D had a lower rate of fat than the fish kept in 6L:18D (P < 0.05). The GSI was higher in the group 6L:18D compared with the 18L:6D (P < 0.005). There was no interaction between photoperiod and period of treatment for total leukocyte count, however with 90 d, the total leukocyte count was decreased (P < 0.05). Moreover, decrease of the hematocrit from 58.1% to 46.9% and an increase of lymphocytes from 31.5% to 43.5% had been found in fish kept in 18L:6D after 90 d (P < 0.05). Fish kept in 6L:18D presented an increase in segmented neutrophils and lymphocytes (P < 0.05). It is concluded that photoperiod maintained at 6L:18D for Piracanjubas permits a greater gonadal development and an amount of immune cells over the photoperiod maintained in 18L: 6D.

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Ghrelin stimulation of gonadotropin (LH) release from goldfish pituitary cells: Presence of the growth hormone secretagogue receptor (GHS-R1a) and involvement of voltage-sensitive Ca2+ channels

Grey

Grayfer

Belosevic

et al. 2010

Molecular and Cellular Endocrinology

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PACAP stimulation of maturational gonadotropin secretion in goldfish involves extracellular signal-regulated kinase, but not nitric oxide or guanylate cyclase, signaling

Cited by 4 publications

References 66 publications

Nitric Oxide and Guanylate Cyclase Signalling are Differentially Involved in Gonadotrophin (LH) Release Responses to Two Endogenous GnRHs from Goldfish Pituitary Cells

Nitric Oxide and Guanylate Cyclase Signalling are Differentially Involved in Gonadotrophin (LH) Release Responses to Two Endogenous GnRHs from Goldfish Pituitary Cells

Influence of Photoperiod over Morphometric and Hematological Parameters of Juvenile Piracanjubas (Brycon orbygnianus)

Ghrelin stimulation of gonadotropin (LH) release from goldfish pituitary cells: Presence of the growth hormone secretagogue receptor (GHS-R1a) and involvement of voltage-sensitive Ca2+ channels

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