Ovarian tissue thawing: A comparison of two conditions

Perdrix, A.; Macé, Bertrand; Milazzo, Jean-Pierre; Liard-Zmuda, A.; Baron, Marc; Rives, Nathalie

doi:10.1016/j.fertnstert.2009.07.967

Cited by 13 publications

(7 citation statements)

References 32 publications

(55 reference statements)

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“…It may seem paradoxical that there was no significant difference in the number of antral follicles between the two groups, but antral follicles were significantly apoptotic on apoptotic assay. A main reason for this is that water content is much higher in antral follicles, leading to intolerance of cryopreservation; another explanation may be that oocyte cytoplasm staining varied from one slice to another, which influences the analysis [ 21 – 23 ].…”

Section: Discussionmentioning

confidence: 99%

Successful fertility following optimized perfusion and cryopreservation of whole ovary and allotransplantation in a premature ovarian insufficiency rat model

Ding

Shao

et al. 2018

J Ovarian Res

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BackgroundFertility preservation by whole ovary cryopreservation and transplantation (WOCP&TP) with vascular anastomosis requires successful cryopreservation. In this study, we investigated the possibility of restoring ovarian function and natural fertility after WOCP&TP in a premature ovarian insufficiency (POI) rat model. The influence of cryopreservation on the offspring of rats following WOCP&TP was also explored.MethodRats aged 8-10 weeks were used as donors and recipients for allotransplantation. Fifteen rat whole ovaries were divided into three groups: the optimized group, the conventional group, and the fresh group. Different perfusion modes were used before cryopreservation and after thawing. Whole ovaries were observed by morphologic analysis, immunohistochemical staining, and transferase-mediated deoxyuridine triphosphate nick end-labeling assay. Ovarian function and fertility after WOCP&TP were then observed in 25 cyclophosphamide-induced POI rats for 8 months. Ovarian function was assessed by vaginal smears and blood hormone levels. Fertility restoration was quantified as the live birth rate after mating. The filial generation of rats was mated at 8-10 weeks of age. Offspring were observed for birth defect.ResultsHistological evaluation demonstrated intact morphology of follicles in all groups, with 77.6% of the total number of follicles identified as intact in the optimized group. The apoptotic rates of ovarian cells in the optimized group were significantly lower than that in the conventional group. Of the 20 live POI rats, 14 (70%) began to recover ovarian function after 2 weeks of transplantation, with normal hormone levels achieved 4 weeks after transplantation. Four of 14 rats were pregnant and delivered live offspring. One rat had a second pregnancy and delivered a second litter of live offspring. When the offspring matured, they were mated, and second and third generations of rats were born. All offspring had no abnormalities in appearance.ConclusionsHigh rates of restoration of ovarian function and natural fertility with multiple generations of offspring were obtained following WOCP&TP in a cyclophosphamide-induced POI rat model by utilizing optimized perfusion. Cryopreservation did not affect the viability of successive generations.

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Section: Discussionmentioning

confidence: 99%

Successful fertility following optimized perfusion and cryopreservation of whole ovary and allotransplantation in a premature ovarian insufficiency rat model

Ding

Shao

et al. 2018

J Ovarian Res

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“…Although cryopreservation consists of freezing and thawing procedures, the latter has been neglected, as there are no standard thawing protocols for ovarian tissue. Recently, two thawing protocols (short term and long -term) have been tested (5). However, both have been performed at the same temperature (30 C).…”

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confidence: 99%

Histologic and ultrastructural features of cryopreserved ovine ovarian tissue: deleterious effect of 1,2-propanediol applying different thawing protocols

Oskam

Asadi

Santos

2010

Fertility and Sterility

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“…Previous studies also reported that the vacuolization of cytoplasm in oocyte appeared in both the fresh and the frozen-thawed groups analyzed by HE stain [34,35]. It seems that this phenomenon happened in the fixation and the stain procedures.…”

Section: Discussionmentioning

confidence: 73%

Cryopreservation of whole ovaries with vascular pedicles: vitrification or conventional freezing?

Zhang

Yan

Cao

et al. 2011

J Assist Reprod Genet

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Ovarian tissue thawing: A comparison of two conditions

Cited by 13 publications

References 32 publications

Successful fertility following optimized perfusion and cryopreservation of whole ovary and allotransplantation in a premature ovarian insufficiency rat model

Successful fertility following optimized perfusion and cryopreservation of whole ovary and allotransplantation in a premature ovarian insufficiency rat model

Histologic and ultrastructural features of cryopreserved ovine ovarian tissue: deleterious effect of 1,2-propanediol applying different thawing protocols

Cryopreservation of whole ovaries with vascular pedicles: vitrification or conventional freezing?

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