Successful fertility following optimized perfusion and cryopreservation of whole ovary and allotransplantation in a premature ovarian insufficiency rat model

Ding, Yan; Shao, Jianhua; Li, Junwei; Zhang, Ying; Hong, K.; Hua, Keqin; Wang, Xiang

doi:10.1186/s13048-018-0401-4

Cited by 17 publications

(12 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Vascular transplantation of fresh whole ovaries has been largely successful in animal models (rats [ 103 , 104 , 105 ], rabbits [ 106 , 107 , 108 , 109 , 110 , 111 , 112 , 113 , 114 ], sheep [ 47 , 49 , 67 , 68 , 78 , 115 , 116 , 117 , 118 , 119 , 120 , 121 ], pigs [ 122 , 123 ], dogs [ 124 ] and monkeys [ 125 , 126 ]). Encouraging results have also been reported on transplantation of frozen–thawed whole ovaries in rats [ 66 , 127 , 128 , 129 ], rabbits [ 130 ] and sheep [ 38 , 40 , 41 , 48 , 49 , 50 , 67 , 68 , 78 , 95 , 102 , 131 ], which will be detailed further in this review. In humans, however, only fresh whole ovary transplantation has been undertaken so far [ 51 , 132 , 133 , 134 ], yielding one live birth to date [ 51 ].…”

Section: Challenges and Solutionsmentioning

confidence: 99%

“…Recently, the efficacy of whole ovary cryopreservation and transplantation was investigated in a POI rat model, simulating female patients with cancer treated by chemotherapy [ 129 ]. POI was induced in 25 recipient rats by administering cyclophosphamide and evidenced by rising serum FSH levels and decreasing progesterone and estradiol values.…”

Section: Challenges and Solutionsmentioning

confidence: 99%

“…In the six animals that did not recover ovarian function, the grafted ovaries and vascular pedicle were atrophic or absent, with severe adhesions, based on postmortem examination. All in all, these results are encouraging and highlight the need for protocols to minimize postoperative inflammatory effects, adhesions, and thrombosis before human trials can commence [ 129 ].…”

Section: Challenges and Solutionsmentioning

confidence: 99%

See 2 more Smart Citations

Whole Ovary Cryopreservation and Transplantation: A Systematic Review of Challenges and Research Developments in Animal Experiments and Humans

Hossay

Donnez²,

Dolmans

2020

JCM

View full text Add to dashboard Cite

Ovarian tissue cryopreservation and transplantation is the only fertility preservation option that enables both restoration of fertility and resumption of ovarian endocrine function, avoiding the morbidity associated with premature menopause. It is also the only technique available to prepubertal patients and those whose treatment cannot be delayed for life-threatening reasons. Ovarian tissue cryopreservation can be carried out in two different ways, either as ovarian cortical fragments or as a whole organ with its vascular pedicle. Although use of cortical strips is the only procedure that has been approved by the American Society for Reproductive Medicine, it is fraught with drawbacks, the major one being serious follicle loss occurring after avascular transplantation due to prolonged warm ischemia. Whole ovary cryopreservation involves vascular transplantation, which could theoretically counteract the latter phenomenon and markedly improve follicle survival. In theory, this technique should maintain endocrine and reproductive functions much longer than grafting of ovarian cortical fragments. However, this procedure includes a number of critical steps related to (A) the level of surgical expertise required to accomplish retrieval of a whole ovary with its vascular pedicle, (B) the choice of cryopreservation technique for freezing of the intact organ, and (C) successful execution of functional vascular reanastomosis upon thawing. The aim of this systematic review is to shed light on these challenges and summarize solutions that have been proposed so far in animal experiments and humans in the field of whole ovary cryopreservation and transplantation.

show abstract

Section: Challenges and Solutionsmentioning

confidence: 99%

Section: Challenges and Solutionsmentioning

confidence: 99%

Section: Challenges and Solutionsmentioning

confidence: 99%

See 1 more Smart Citation

Whole Ovary Cryopreservation and Transplantation: A Systematic Review of Challenges and Research Developments in Animal Experiments and Humans

Hossay

Donnez²,

Dolmans

2020

JCM

View full text Add to dashboard Cite

show abstract

“…There are two UTx groups in Shanghai. Hua and Liu’s group at the Obstetrics and Gynecology Hospital (Fudan University) has done extensive research on preservation of female fertility [24,25]. This group has treated a significant number of patients with genital malformation who wanted to have their own child, and are committed to extending UTx to the clinical stage [26,27].…”

Section: Basic Utx Researchmentioning

confidence: 99%

Current Progress in Uterus Transplantation Research in Asia

Kisu

Liu

Chen

et al. 2019

JCM

View full text Add to dashboard Cite

Uterus transplantation (UTx) is now a possible approach for women with absolute uterine factor infertility to deliver a child, following the first successful delivery by Brännström et al. in Sweden in September 2014. This remarkable achievement attracted major attention worldwide and caused many countries to prepare for UTx, including countries in Asia. To date, three groups have performed UTx in humans in Asia, and many others are aiming for the clinical application of UTx with accumulation of basic experimental data. Therefore, it is likely that UTx will expand rapidly in Asia in the near future, although this will depend on ethical, social and religious views in each country. With this background, herein we summarize the current progress of UTx in East, Southeast and South Asia, with the purpose of increasing understanding of the current status of basic and clinical UTx research in each country and sharing progress and knowledge to ensure future development of UTx research in Asia.

show abstract

“…As many studies have confirmed, the influence of the freezing time on cell viability is negligible. In our previous studies, short-term cryopreserved ovaries [ 8 , 9 ] and bladder mucosa [ 10 ] survived in the transplanted subjects. Moreover, various tissues, such as cartilage [ 11 ] and heart valves [ 12 , 13 ], have been successfully cryopreserved and banked for more than 6 months and achieved transplantation effects that were equivalent to those of fresh controls.…”

Section: Introductionmentioning

confidence: 99%

Biobanked human foreskin epithelial cell sheets reduce inflammation and promote wound healing in a nude mouse model

et al. 2021

Self Cite

View full text Add to dashboard Cite

Background Human epithelial cell sheets (ECSs) are used to clinically treat epithelial conditions such as burns, corneal blindness, middle ear cholesteatoma and vitiligo. As a widely used material in clinic, there is little information on the biobanking of ECSs and its repair effect after storage. Results Two methods for biobanking foreskin ECSs were compared in a short term (7 days): 4-degree storage and programmed cryopreservation. Cell sheet integrity, viability, apoptosis, immunogenicity, mechanical properties and function were evaluated. In vivo, ECSs were directly transplanted to skin defect models and histological examination was performed at 1 week postoperatively. We successfully extracted human foreskin-derived primary epithelial cells and fabricated them into ECSs. Compared with 4-degree storage, programmed cryopreservation preserved the ECS structural integrity, enhanced the mechanical properties, decreased HLA-I expression, and increased cell viability and survival. An increased proportion of melanocytes with proliferative capacity remained in the cryopreserved sheets, and the undifferentiated epithelial cells were comparable to those of the fresh sheets. In vivo, cryopreserved ECSs could reduce inflammatory cell infiltration and promote connective tissue remodeling, epithelial cell proliferation and vascular regeneration. Conclusions Programmed cryopreservation of ECSs was superior and more feasible than 4-degree storage and the cryopreserved ECSs achieved satisfying skin wound healing in vivo. We anticipate that the off-the-shelf ECSs could be quickly used, such as, to repair human epithelial defect in future. Graphical abstract

show abstract

Successful fertility following optimized perfusion and cryopreservation of whole ovary and allotransplantation in a premature ovarian insufficiency rat model

Cited by 17 publications

References 32 publications

Whole Ovary Cryopreservation and Transplantation: A Systematic Review of Challenges and Research Developments in Animal Experiments and Humans

Whole Ovary Cryopreservation and Transplantation: A Systematic Review of Challenges and Research Developments in Animal Experiments and Humans

Current Progress in Uterus Transplantation Research in Asia

Biobanked human foreskin epithelial cell sheets reduce inflammation and promote wound healing in a nude mouse model

Contact Info

Product

Resources

About