Cryopreservation of whole ovaries with vascular pedicles: vitrification or conventional freezing?

Zhang, Jianmin; Yan, Sheng; Cao, Yongzhi; Wang, Hongyan; Chen, Zi‐Jiang

doi:10.1007/s10815-011-9539-3

Cited by 27 publications

(24 citation statements)

References 42 publications

(54 reference statements)

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“…Sugimoto et al (2) found that eight out of nine animals that had received transplanted vitrified ovary reestablished their cyclicity; histology revealed healthy antral follicles 3 months after transplantation. In other experiments on vitrification of whole ovaries in the sheep (41) and cow (42), some success have been reported. However, with human ovarian strips, the efficacy of slow-freezing versus vitrification is controversial (43,44).…”

Section: Discussionmentioning

confidence: 98%

Viability and function of the cryopreserved whole rat ovary: comparison between slow-freezing and vitrification

Milenković

Díaz-García

Wallin

et al. 2012

Fertility and Sterility

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Section: Discussionmentioning

confidence: 98%

Viability and function of the cryopreserved whole rat ovary: comparison between slow-freezing and vitrification

Milenković

Díaz-García

Wallin

et al. 2012

Fertility and Sterility

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“…Some studies compare Fig. 4 a, b The stromal cell density in different groups vitrification versus slow-cooling by comparing parameters such as follicle viability, histological examination and apoptosis assessment, or hormone concentrations in the culture supernatants, but no consensus has yet been reached [23,24]. Having analyzed the preexperimental data combined in previous studies, we chose slow-cooling in our study, since it enables the precise adjustment of the temperature gradients to achieve an accurate cooling rate through the entire ovary and leads to less ice formation.…”

Section: Discussionmentioning

confidence: 99%

Successful cryopreservation of whole sheep ovary by using DMSO-free cryoprotectant

Lan

Zhao

et al. 2015

J Assist Reprod Genet

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Purpose The study aims to assess the protective effects of dimethyl sulfoxide (DMSO)-free solution based on trehalose on the cryopreservation of a whole sheep ovary and evaluate its use as an efficient cryoprotectant. Method Twenty-one ovaries collected from 6-to 8-month-old non-pregnant female sheep were randomly distributed into three groups, namely, a fresh group, a DMSO-free group, and a DMSO group. The morphology, cell apoptosis (by hematoxylin and eosin (HE) staining and terminal deoxynucleotidyltransferasemediated dUTP nick-end labeling (TUNEL) assay), and mRNA transcript of Bcl-2-associated X protein (BAX) and cold inducible RNA-binding protein (CIRP) (by real-time PCR) of the thawed sheep ovaries and fresh controls were tested to establish a criterion for appraising the results of the cryopreservation.Results (i) The histological assessment indicated that the structure of the DMSO-free ovaries remained largely intact and comparable to those of the fresh control groups; whereas, significant damage was observed in the ovaries of the DMSO group (P<0.05). (ii) The TUNEL assay and mRNA transcript of the BAX assessment showed that the apoptosis parameter in the fresh group was the lowest among all the groups (P<0.05), and the parameter in the DMSO-free group was significantly lower than that in the DMSO group (P<0.05). (iii) The level of the CIRP transcripts increased the most in the DMSO-free group followed by the DMSO group and the fresh control group (P<0.05). Conclusions These results indicate that a DMSO-free cryoprotectant solution, especially a trehalose cryoprotectant, is an efficient cryoprotectant and has a beneficial effect on the cryopreservation of whole sheep ovaries.Capsule These results indicate that a DMSO-free and trehalose-containing cryoprotectant solution has a beneficial effect on the cryopreservation of intact sheep ovaries.Tianqi Du and Lan Chao contributed equally to this work.

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“…In sheep studies, most follicular population was lost because of vascular complications after transplantation with vascular anastomosis [16,17,23], although a successful pregnancy and an ovarian function of 6 years were reported after whole ovary cryopreservation and autotransplantation in sheep [12,32]. In cow study, the results were also not encouraging [24,33]. To date, it has not been possible to perform whole ovary transplantation after cryopreservation in humans because of technical difficulties in cryopreservation.…”

Section: Discussionmentioning

confidence: 99%

“…The procedure was carried out according to the protocol that we previously reported [24]. Warming of ovary One month later, the ovaries were removed from the Dewar and held in air at room temperature for 2 min before plunging in a water bath at 37°C for another 2 min with gentle shaking.…”

Section: Ovarian Perfusion and Cryopreservation Of Ovarymentioning

confidence: 99%

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Optimizing cryoprotectant perfusion conditions for intact ovary: a bovine model

Zhang

Ruan

et al. 2012

J Assist Reprod Genet

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Purpose The aim of this study was to detect the effects of different perfusion pressure and different length of perfusion period on whole ovarian cryopreservation Methods Bovine whole ovaries were vitrified-warmed. The ovaries were divided into the experimental groups according to different perfusion pressure and different length of perfusion period. Follicular viability was assessed using the trypan blue test; the percentage of morphologically normal primordial follicles and the 17-β estradiol level in the culture supernatants were measured. Results When perfusion pressure was 100 mmHg, and the length of perfusion period was 40 min, the viability of ovarian tissues in bovine whole ovarian cryopreservation were higher than other protocols. Conclusion Protocol IIb (the perfusion pressure was 100 mmHg, and the length of perfusion period was 40 min) was appropriate for bovine whole ovarian cryopreservation.

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Cryopreservation of whole ovaries with vascular pedicles: vitrification or conventional freezing?

Abstract: Vitrification seems to be more suitable than conventional freezing for cryopreservation of whole ovaries. However, further studies are required to improve the efficacy of vitrifying whole ovaries.

Cited by 27 publications

References 42 publications

Viability and function of the cryopreserved whole rat ovary: comparison between slow-freezing and vitrification

Viability and function of the cryopreserved whole rat ovary: comparison between slow-freezing and vitrification

Successful cryopreservation of whole sheep ovary by using DMSO-free cryoprotectant

Optimizing cryoprotectant perfusion conditions for intact ovary: a bovine model

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