Histologic and ultrastructural features of cryopreserved ovine ovarian tissue: deleterious effect of 1,2-propanediol applying different thawing protocols

Oskam, Irma C.; Asadi, Babak A.; Santos, Regiane R.

doi:10.1016/j.fertnstert.2010.02.003

Cited by 22 publications

(17 citation statements)

References 11 publications

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“…The results of the present study show follicular loss as well as major damage of the stromal tissue, in compliance with the results from our previous study (Oskam et al. 2010), as well as other groups (Marsella et al.…”

Section: Discussionsupporting

confidence: 94%

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Irreversible Damage in Ovine Ovarian Tissue after Cryopreservation in Propanediol: Analyses after In Vitro Culture and Xenotransplantation

Oskam¹,

Lund

Santos

2011

Reprod Domestic Animals

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Current progress in cancer treatment has increased the incidence of long-term patient survival. Ovarian tissue cryopreservation (OT) is still the most promising fertility saving method offered to young female patients with cancer prior to the onset of radio-chemotherapy. Further follicular development of immature primordial follicles depends on transplantation or in vitro culture (IVC). Aim of this study was to evaluate the appropriateness of cryopreserved ovine OT with 1,2-propanediol (PROH) after short-term IVC and xenotransplantation (XT). Ovarian tissue fragments from young adult sheep were cryopreserved using a standard slow-freezing protocol with 1.5 M PROH. Cryopreserved OT was assessed by light- and transmission electron microscopic analyses after thawing, IVC or XT in severe immunodeficient mice. Control OT showed the presence of healthy preantral follicles (Mean: 78.8%; SE 2.9%) and normal structure of the stromal tissue. After thawing and IVC over 80% of damaged primordial follicles and poor preservation of the stromal tissue was observed. After XT, OT demonstrated deficient follicles and huge areas of vacuolization in the stromal tissue confirmed by ultrastructural assessment. In conclusion, because of the irreversible character of the follicular and stromal damage of cryopreserved ovine ovarian tissue after IVC and XT, strong improvement of the utilized protocol is needed to be suitable for the preservation of ovine ovarian tissue. The deleterious effects of PROH do not imply its exclusion as cryoprotectant, but more research is needed for the development of less toxic cryoprotectant mixtures and toxicity neutralizers with attested cryoprotectant capacity for the safe and feasible freezing of human ovarian tissue.

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Section: Discussionsupporting

confidence: 94%

“…2008). Deleterious effects related to PROH were recently confirmed by a study performed at our laboratory with ovine ovarian cortex fragments (Oskam et al. 2010).…”

Section: Introductionmentioning

confidence: 74%

Irreversible Damage in Ovine Ovarian Tissue after Cryopreservation in Propanediol: Analyses after In Vitro Culture and Xenotransplantation

Oskam¹,

Lund

Santos

2011

Reprod Domestic Animals

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“…Vacuolization has been reported after cryopreservation of ovarian tissue from sheep (Santos et al 2006b;Oskam et al 2010), bovine (Celestino et al 2008) and human (Marsella et al 2008), with indications of damage in the ER (Silva et al 2000). In agreement with this evidence, Oskam et al (2010) found oocyte vacuolization together with an extreme decrease in or even total absence of smooth and rough ER in cryopreserved sheep ovarian tissue.…”

Section: Discussionsupporting

confidence: 67%

“…Similarly, previous studies have shown the value of antioxidants in protecting ovarian tissue from ischemia following transplantation (Kim et al 2004) or their usefulness in freezing medium to improve follicular preservation (Melo et al 2011;Sanfilippo et al 2013). Vacuolization has been reported after cryopreservation of ovarian tissue from sheep (Santos et al 2006b;Oskam et al 2010), bovine (Celestino et al 2008) and human (Marsella et al 2008), with indications of damage in the ER (Silva et al 2000). In agreement with this evidence, Oskam et al (2010) found oocyte vacuolization together with an extreme decrease in or even total absence of smooth and rough ER in cryopreserved sheep ovarian tissue.…”

Section: Discussionmentioning

confidence: 79%

Vitamin E-analog Trolox prevents endoplasmic reticulum stress in frozen-thawed ovarian tissue of capuchin monkey (Sapajus apella)

Brito

Scalercio

et al. 2013

Cell Tissue Res

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Ovarian fragments were exposed to 0.5 M sucrose and 1 M ethylene glycol (freezing solution; FS) with or without selenium or Trolox. Histological and ultrastructural analyses showed that the percentages of normal follicles in control tissue and in tissue after exposure to FS+50 μM Trolox were similar. Trolox prevented endoplasmic reticulum (ER)-related vacuolization, which is commonly observed in oocytes and stromal tissue after exposure to FS. From the evaluated stress markers, superoxide dismutase 1 (SOD1) was up-regulated in ovarian tissue exposed to FS+10 ng/ml selenium. Ovarian fragments were subsequently frozenthawed in the presence of FS with or without 50 μM Trolox, followed by in vitro culture (IVC). Antioxidant capacity in ovarian fragments decreased after freeze-thawing in Troloxfree FS compared with FS+50 μM Trolox. Although freezing itself minimized the percentage of viable follicles in each solution, Trolox supplementation resulted in higher rates of viable follicles (67 %), even after IVC (61 %). Furthermore, stress markers SOD1 and ERp29 were up-regulated in ovarian tissue frozen-thawed in Trolox-free medium. Relative mRNA expression of growth factors markers was evaluated after freeze-thawing followed by IVC. BMP4, BMP5, CTGF, GDF9 and KL were down-regulated independently of the presence of Trolox in FS but down-regulation was less pronounced in the presence of Trolox. Thus, medium supplementation with 50 μM Trolox prevents ER stress and, consequently, protects ovarian tissue from ER-derived cytoplasmic vacuolization. ERp29 but not ERp60, appears to be a key marker linking stress caused by freezing-thawing and cell vacuolization.

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“…In a recent study by our team, ovarian tissue of goats was successfully cryopreserved in the presence of 1.0 M PROH (unpublished data). In contrast, Oskam et al [29], reported that PROH caused deleterious effects to the stroma and cryopreserved PAFs of ovine ovarian tissue.…”

Section: Discussionmentioning

confidence: 91%

Ultrastructural features of agouti (Dasyprocta aguti) preantral follicles cryopreserved using dimethyl sulfoxide, ethylene glycol and propanediol

Wanderley¹,

Luz²,

Faustino³

et al. 2012

Theriogenology

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].You may download, copy and otherwise use the AAM for non-commercial purposes provided that your license is limited by the following restrictions:(1) You may use this AAM for non-commercial purposes only under the terms of the CC-BY-NC-ND license.(2) The integrity of the work and identification of the author, copyright owner, and publisher must be preserved in any copy. Ultrastructural features of agouti (Dasyprocta aguti) preantral follicles cryopreserved using dimethyl sulfoxide, ethylene glycol and propanediol AbstractThe objective was to develop an efficient protocol for cryopreservation of agouti (Dasyprocta aguti) ovarian tissue. Agouti ovarian fragments were placed, for 10 min, in a solution containing MEM and fetal bovine serum plus 1.5 M dimethyl sulfoxide (DMSO), ethylene glycol (EG) or propanediol (PROH); some of those fragments were subsequently cryopreserved in a programmable freezer. After exposure and/or thawing, all samples were fixed in Carnoy prior to histological analysis. To evaluate ultrastructure, follicles from the control and all cryopreserved treatments were fixed in Karnovsky and processed for transmission electron microscopy. After exposure and freezing, there was a significant decrease in the percentage of morphologically normal preantral follicles in all treatments when compared to the control (92.67 ± 2.79, mean ± SD). However, there were no significant difference when the exposure and freezing procedures were compared using the same cryoprotectant. Moreover, there was no significant difference among cryoprotectants at the time of exposure (DMSO: 64.7 ± 3.8; EG: 70.7 ± 11.2, PROH: 63.3 ± 8.5) or after freezing (DMSO: 60.6 ± 3.6, EG: 64.0 ± 11.9; PROH: 62.0 ± 6.9). However, only follicles frozen with PROH had normal ultrastructure. In conclusion, preantral follicles enclosed in agouti ovarian tissue were successfully cryopreserved using 1.5 M PROH, with satisfactory maintenance of follicle morphology and ultrastructure.

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Histologic and ultrastructural features of cryopreserved ovine ovarian tissue: deleterious effect of 1,2-propanediol applying different thawing protocols

Cited by 22 publications

References 11 publications

Irreversible Damage in Ovine Ovarian Tissue after Cryopreservation in Propanediol: Analyses after In Vitro Culture and Xenotransplantation

Irreversible Damage in Ovine Ovarian Tissue after Cryopreservation in Propanediol: Analyses after In Vitro Culture and Xenotransplantation

Vitamin E-analog Trolox prevents endoplasmic reticulum stress in frozen-thawed ovarian tissue of capuchin monkey (Sapajus apella)

Ultrastructural features of agouti (Dasyprocta aguti) preantral follicles cryopreserved using dimethyl sulfoxide, ethylene glycol and propanediol

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