2013
DOI: 10.1007/s00441-013-1764-x
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Abstract: Ovarian fragments were exposed to 0.5 M sucrose and 1 M ethylene glycol (freezing solution; FS) with or without selenium or Trolox. Histological and ultrastructural analyses showed that the percentages of normal follicles in control tissue and in tissue after exposure to FS+50 μM Trolox were similar. Trolox prevented endoplasmic reticulum (ER)-related vacuolization, which is commonly observed in oocytes and stromal tissue after exposure to FS. From the evaluated stress markers, superoxide dismutase 1 (SOD1) wa… Show more

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Cited by 19 publications
(9 citation statements)
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“…[ 32 ] The 50 μmol/L trolox protected ovarian tissue of capuchin monkey from endoplasmic reticulum-derived cytoplasmic vacuolization. [ 33 ] Addition of 40 μmol/L trolox in the cryopreservation media improved the postthawed human semen quality. [ 34 ] The 60 μmol/L and 120 μmol/L presented greater structural integrity, including membrane and mitochondria and kinematics for ram spermatozoa after cryopreservation.…”
Section: Discussionmentioning
confidence: 99%
“…[ 32 ] The 50 μmol/L trolox protected ovarian tissue of capuchin monkey from endoplasmic reticulum-derived cytoplasmic vacuolization. [ 33 ] Addition of 40 μmol/L trolox in the cryopreservation media improved the postthawed human semen quality. [ 34 ] The 60 μmol/L and 120 μmol/L presented greater structural integrity, including membrane and mitochondria and kinematics for ram spermatozoa after cryopreservation.…”
Section: Discussionmentioning
confidence: 99%
“…UPR is activated via one of the three membrane-bound transducing receptors (ATF6, PERK, IRE1α), these three sensors thus constitute the three branches of the UPR signaling pathways [85,86]. It was observed that SOD1 and the ER stress marker ERP29 gene expression were significantly up-regulated in response to freeze-thaw stress in primate ovarian tissue [81]. In yeast, genes expression for protein chaperones such as SSA4, HSP26, HSP42 were found to be up-regulated in response to freeze-thaw stress when cells were frozen in the absence of cryoprotectants [87].…”
Section: Cryopreservation and Ros Productionmentioning
confidence: 99%
“…The use of Trolox, a water-soluble analog of vitamin E, increased antioxidant capacity, prevented ER stress and improved the viability of ovarian tissues. This indicates a role for both oxidative stress and ER stress during cryopreservation [81]. Intriguingly, it was found that an inhibitor that prevents ER stress-induced apoptosis, Salubrinal, did not improve development and viability of bovine blastocyst [180], indicating that preventing ER stress-induced cell death alone may not be sufficient to prevent cryopreservation-induced damage.…”
Section: Effectiveness Of Antioxidants In Preventing Cryoinjury: Lessmentioning
confidence: 99%
“…Bozkurt et al (2012) had found a protective effect for selenium on ischemia reperfusion injury in rat ovary via studying oxidative stress indices [ 30 ]. Brito et al (2013) showed protective effects of selenium in combination with vitamin E on ovarian samples of monkey via investigating expression of superoxide dismutase 1 gene [ 31 ]. In general, protective effect of selenium has been observed in many studies like our study.…”
Section: Discussionmentioning
confidence: 99%