Osteogenic differentiation of human bone marrow mesenchymal stem cells in hydrogel containing nacre powder

Flausse, Alicia; Henrionnet, Christel; Dossot, Manuel; Dumas, Dominique; Hupont, Sébastien; Pinzano, Astrid; Mainard, Didier; Galois, Laurent; Magdalou, Jacques; Lopez, Évelyne; Gillet, Pierre; Rousseau, Marthe

doi:10.1002/jbm.a.34629

Cited by 30 publications

(34 citation statements)

References 34 publications

(55 reference statements)

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“…These two types of cells could be seeded in a composite scaffold enriched with nacre. Flausse et al have demonstrated the efficiency of powdered nacre in stimulation of differentiation and bone matrix production of MSCs seeded in alginate hydrogel. Powdered nacre was still used in an injectable form (mixed with venous blood) to fill bone defects in vertebral and maxillar sites of sheep .…”

Section: Discussionmentioning

confidence: 99%

The effect of nacre extract on cord blood‐derived endothelial progenitor cells: A natural stimulus to promote angiogenesis?

Willemin

Zhang

Velot

et al. 2019

J Biomedical Materials Res

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Angiogenesis is a critical parameter to consider for the development of tissue‐engineered bone substitutes. The challenge is to promote sufficient vascularization in the bone substitute to prevent cell death and to allow its efficient integration. The capacity of nacre extract to restore the osteogenic activity of osteoarthritis osteoblasts has already been demonstrated. However, their angiogenic potential on endothelial progenitor cells (EPCs) was not yet explored. Therefore, the current study aimed at investigating if nacreous molecules affect EPC behavior. The gene and protein expression levels of endothelial cell (EC)‐specific markers were determined in EPCs cultivated in presence of a nacre extract (ethanol soluble matrix [ESM] at two concentrations: 100 μg/mL and 200 μg/mL (respectively abbreviated ESM100 and ESM200)). Cell functionality was explored by proangiogenic factors production and in vitro tube formation assay. ESM200 increased the expression of some EC‐specific genes. The in vitro tube formation assay demonstrated that ESM200 stimulated tubulogenesis affecting angiogenic parameters. We demonstrated that a stimulation with 200 μg/mL of ESM increased angiogenesis key elements. This in vitro study strongly highlights the proangiogenic effect of ESM. Due to its osteogenic properties, previously demonstrated, ESM could constitute the key element to develop an ideal prevascularized bone substitute. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2019.

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Section: Discussionmentioning

confidence: 99%

The effect of nacre extract on cord blood‐derived endothelial progenitor cells: A natural stimulus to promote angiogenesis?

Willemin

Zhang

Velot

et al. 2019

J Biomedical Materials Res

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“…In a recent study antioxidant peptides from P. fucata were found to inhibit the photoaging effect in mice [64]. In addition, enhanced osteogenic differentiation in hydrogel containing nacre powder suggested possible use of nacre in various future tissue-engineering applications [65]. Furthermore, the diversity of shell matrix proteins may be implicated in various biological activities [66].…”

Section: Discussionmentioning

confidence: 99%

Water Soluble Bioactives of Nacre Mediate Antioxidant Activity and Osteoblast Differentiation

2013

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The water soluble matrix of nacre is a proven osteoinductive material. In spite of the differences in the biomolecular compositions of nacre obtained from multiple species of oysters, the common biochemical properties of those principles substantiate their biological activity. However, the mechanism by which nacre stimulates bone differentiation remains largely unknown. Since the positive impact of antioxidants on bone metabolism is well acknowledged, in this study we investigated the antioxidant potential of a water soluble matrix (WSM) obtained from the nacre of the marine oyster Pinctada fucata, which could regulate its osteoblast differentiation activity. Enhanced levels of ALP activity observed in pre-osteoblast cells upon treatment with WSM, suggested the induction of bone differentiation events. Furthermore, bone nodule formation and up-regulation of bone differentiation marker transcripts, i.e. collagen type-1 and osteocalcin by WSM confirmed its ability to induce differentiation of the pre-osteoblasts into mature osteoblasts. Remarkably, same WSM fraction upon pre-treatment lowered the H2O2 and UV-B induced oxidative damages in keratinocytes, thus indicating the antioxidant potential of WSM. This was further confirmed from the in vitro scavenging of ABTS and DPPH free radicals and inhibition of lipid peroxidation by WSM. Together, these results indicate that WSM poses both antioxidant potential and osteoblast differentiation property. Thus, bioactivities associated with nacre holds potential in the development of therapeutics for bone regeneration and against oxidative stress induced damages in cells.

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“…fabricated Alg beads with nacre (mother of pearl) powder and culture them with BMSC cells. Observation of histochemistry suggested the formation of a new tissue composed of collagen fibers in the presence of nacre and HAp crystals in the Alg beads . Cell attachment and mineral deposition were enhanced when the sphere containing β‐TCP supplemented with silica and silicatein into PLGA MS. Mineralization increased from 0.29 ± 0.06 in β‐TCP micro to 0.51 ± 0.09 in addition to silica.…”

Section: Composite Microspherementioning

confidence: 99%

Polymeric microspheres: a delivery system for osteogenic differentiation

Patel

Kwak

et al. 2017

Polymers for Advanced Techs

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Advanced drug delivery systems employing controlled release technology are being developed to address many of the difficulties associated with traditional methods of drug administration. Controlled release technology involves the use of devices such as polymer‐based disks, rods, pellets, or microspheres (MSs) that encapsulate drugs, genes, cytokines, and growth factors and release them in specific location within the body in a controlled fashion, for relatively long periods of time. Among these, microencapsulation is one of the core technologies used in polymer‐based drug delivery systems. In this regard, MS serves as microcarriers for sustain drug release facilitating their use for invasive or minimally invasive treatment. MS has significant potential for the application in bone repair, intra‐articular treatment of osteoarthritis, and biological bone growth. The present review compiles the recent advances in polymeric MS for application in bone and cartilage regeneration. Copyright © 2017 John Wiley & Sons, Ltd.

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Osteogenic differentiation of human bone marrow mesenchymal stem cells in hydrogel containing nacre powder

Cited by 30 publications

References 34 publications

The effect of nacre extract on cord blood‐derived endothelial progenitor cells: A natural stimulus to promote angiogenesis?

The effect of nacre extract on cord blood‐derived endothelial progenitor cells: A natural stimulus to promote angiogenesis?

Water Soluble Bioactives of Nacre Mediate Antioxidant Activity and Osteoblast Differentiation

Polymeric microspheres: a delivery system for osteogenic differentiation

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