Osteoblast activity of MG-63 cells is enhanced by growth on a lactoferrin-immobilized titanium substrate

Accelerated Osteogenic Differentiation of MC3T3-E1 Cells by Lactoferrin-Conjugated Nanodiamonds through Enhanced Anti-Oxidant and Anti-Inflammatory Effects

“…As expected, MC3T3-E1 cells treated with LF (50 µg)-NDs exhibited higher ALP activity than did cells treated with NDs and LF (10 µg)-NDs at 3 and 7 days. Generally, calcium deposition was measured as a marker of osteogenic differentiation, and upregulation of calcium deposition is a major event that occurs during late time points of osteogenesis [20,32]. Figure 7B shows the in vitro amounts of calcium deposited by MC3T3-E1 cells treated with different samples for different culture times.…”

“…Interestingly, LF is known to have potential as an osteogenic factor. Previous reports have demonstrated that materials functionalized with LF induce osteogenic differentiation of mesenchymal stem cells (MSCs) and adipose-derived stem cells (ADSCs) [19,20]. The mechanism of LF action on osteogenic differentiation stimulates cells via lipoprotein receptor-related protein 1 (LRP1)-independent protein kinase A (PKA) and p38 signaling pathways [21].…”

Fabrication of a BMP-2-immobilized porous microsphere modified by heparin for bone tissue engineering

“…After adhering Hep-DOPA, the N1s and S2p content of Hep-PMS increased compared to PMS alone. Our previous studies demonstrated that PMS, polycaprolactone (PCL) fibers and titanium (Ti) modified with Hep-DOPA showed increased N1s and S2p content compared to PMS, PCL fibers, and Ti only [9,19,34]. Moreover, after immobilizing BMP-2 on PMS and Hep-PMS, N1s and S2p content on PMS and Hep-PMS containing BMP-2 increased compared to that on Hep-PMS.…”

“…However, we did not measure osteoblast activity on the Hep-PMS substrate. Our previous studies demonstrated that MG-63 cells or rabbit adipose-derived stem cells (rADSCs) grown on PMS, PCL fiber, and Ti substrates modified with heparin did not significantly differ in the amount of cell proliferation, ALP activity, the amount of calcium deposited, or in gene expression compared to cells grown on PMS, PCL fibers, and Ti substrates without heparin [9,19,34]. Because these results indicated that heparin does not affect the osteoblast activity, we deferred measuring the osteoblast activity on the Hep-PMS substrate.…”

“…ALP activity is typically used to confirm early differentiation of osteoblast-like cells [9,19,34,42]. The ALP activity of MG-63 cells grown on PMS, BMP-2/PMS, and BMP-2/Hep-PMS were evaluated for culture periods of up to 10 days.…”