Enhancement of growth and osteogenic differentiation of MC3T3-E1 cells via facile surface functionalization of polylactide membrane with chitooligosaccharide based on polydopamine adhesive coating

Li, Huihua; Luo, Chuang; Luo, Binghong; Wen, Wei; Wang, Xiaoying; Ding, Shan; Zhou, Changren

doi:10.1016/j.apsusc.2015.11.077

Cited by 21 publications

(10 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PLA is a thermoplastic polyester synthesized using monomers derived from renewable resources, and has been extensively used in bone tissue engineering, due to its biocompatibility, biodegradability, non-toxicity, good processability and excellent mechanical properties. Moreover, PLA has been approved by Food and Drug Administration (FDA) for clinical use [14,15].The combination of chitosan and PLA can lead to novel multi-functional biomaterials with improved properties, suitable for biomedical applications. The most facile method to obtain such composites is by simply mixing the two polymers, however, the homogeneous mixing of CS with PLA is challenging, due to their dissimilar structure and properties.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Biodegradable Chitosan-graft-Poly(l-lactide) Copolymers For Bone Tissue Engineering

et al. 2020

View full text Add to dashboard Cite

The design and synthesis of new biomaterials with adjustable physicochemical and biological properties for tissue engineering applications have attracted great interest. In this work, chitosan-graft-poly(l-lactide) (CS-g-PLLA) copolymers were prepared by chemically binding poly(l-lactide) (PLLA) chains along chitosan (CS) via the “grafting to” approach to obtain hybrid biomaterials that present enhanced mechanical stability, due to the presence of PLLA, and high bioactivity, conferred by CS. Two graft copolymers were prepared, CS-g-PLLA(80/20) and CS-g-PLLA(50/50), containing 82 wt % and 55 wt % CS, respectively. Degradation studies of compressed discs of the copolymers showed that the degradation rate increased with the CS content of the copolymer. Nanomechanical studies in the dry state indicated that the copolymer with the higher CS content had larger Young modulus, reduced modulus and hardness values, whereas the moduli and hardness decreased rapidly following immersion of the copolymer discs in alpha-MEM cell culture medium for 24 h. Finally, the bioactivity of the hybrid copolymers was evaluated in the adhesion and growth of MC3T3-E1 pre-osteoblastic cells. In vitro studies showed that MC3T3-E1 cells exhibited strong adhesion on both CS-g-PLLA graft copolymer films from the first day in cell culture, whereas the copolymer with the higher PLLA content, CS-g-PLLA(50/50), supported higher cell growth.

show abstract

mentioning

confidence: 99%

mentioning

confidence: 99%

Biodegradable Chitosan-graft-Poly(l-lactide) Copolymers For Bone Tissue Engineering

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Moreover, it could also be found from Figure 11 that the expression of ALP activity of rBMSCs on OPC substrate was remarkably up‐regulated after 21 days of culture, although did not show notable increase at the seventh day of culture, suggesting that cell differentiation could be activated at a later time point on OPC substrate despite there was not better onset of differentiation for rBMSCs. This outcome was probably ascribed to the long‐term interlock generated by mechanical stimuli at the cell‐soft‐solid interface of the OPC, resulting in advancement in cell differentiation 22,23,25 . As we had learned from the rheological characterization that OPC‐based liquid crystalline substrates mainly exhibit viscous properties, imitating the flowable morphology of the natural bio‐membrane surface so that being able to serve as a soft elastic‐solid model 30 that provided appropriate mechanical stimuli mediating cell behavior 11,34,37 …”

Section: Discussionmentioning

confidence: 99%

“…COS is the low molecular weight chitosan and possesses appropriate biological characteristics 22 especially excellent osteogenic activity. COS can significantly upregulate the alkaline phosphatase activity and calcium deposition leading to promotion of the osteogenic differentiation of stem cells, 23 which makes it hold promising potential as bioactive molecules used in bone tissue engineering. Herein, we construct a novel OPC‐based liquid crystalline substrate functionalized with COS based on PDOPA adhesive coating, aim to combine viscoelasticity of LC material and bioactivity of COS into the substrate model to improve biological function of the OPC‐based substrates and provide a fertile ground for stem cell growth and osteogenic differentiation, and explored the synergistic effects of surface chemistry and liquid crystalline feature on the cell behavior.…”

Section: Introductionmentioning

confidence: 99%

Enhanced cell affinity and osteogenic differentiation of liquid crystal‐based substrate via surface bio‐functionalization

Yang

Huang

Jian

et al. 2020

J Biomedical Materials Res

View full text Add to dashboard Cite

Regulation of cell‐substrate interactions is an important factor for modulating cell behaviors. Tailoring the physical and chemical properties of the substrates to better mimic the extracellular matrix (ECM) of native tissue is a more effective strategy for enhancing the cell‐substrate contact. In current work, we aim at improving surface bioactivity based on the liquid crystalline substrates for the enhancement in cell affinity and osteogenic differentiation. Polydopamine (PDOPA) adhesive coating was used as a reactive platform for the immobilization of chitooligosaccharide (COS) on the octyl hydroxypropyl cellulose ester (OPC) substrate to generate active OPC‐PDOPA‐COSs liquid crystalline substrates. Results demonstrated that PDOPA‐coated OPC surfaces showed remarkably improved hydrophility and increased elastic modulus, leading to better initial cell attachment. Subsequent COS immobilization on the OPC‐PDOPA layer could induce promotion of cell proliferation, polarization and cytoskeleton formation. Rat bone marrow mesenchymal stem cells (rBMSCs) seeded on the OPC‐PDOPA‐COSs showed higher alkaline phosphatase (ALP) activity, calcium deposition, and up‐regulated bone‐related genes expression, including BMP‐2, RUNx‐2, COL‐I and OCN. In conclusion, surface biofunctionalization on the OPC‐based liquid crystalline substrates could come into being the appropriate combination of surface chemistry and liquid crystalline characteristic that simulating in vivo ECM environment, resulting in a favorable support to enhance positive cell‐substrate interactions.

show abstract

“…Attachment of Bioactive Materials for Bone Tissue Engineering : Polydopamine coatings have been used to attach biopolymers and modified biopolymers (e.g., lauric acid modified chitosan, carboxylmethyl chitosan, chitosan oligomers, collagen, silk fibroin, and alginate) to polymeric and metallic scaffolds to enhance their biocompatibility and osseointegration. These hybrid materials variously aided the bone tissue scaffold by enhancing the mechanical properties, increasing hydroxyapatite mineralization, providing antibacterial properties, increasing alkaline phosphatase activity, and boosting cytocompatibility, including improved osteoblast adhesion, proliferation, and osteogenic differentiation . Similarly, proteins and a crude extract of a Chinese herbal remedy have been attached to implant materials through polydopamine to improve the cytocompatibility for bone regeneration, providing excellent osteoinductivity and strong bone regeneration performance .…”

Section: Applications Of Catecholamine Polymersmentioning

confidence: 99%

Versatile Surface Modification Using Polydopamine and Related Polycatecholamines: Chemistry, Structure, and Applications

Barclay

Hegab

Clarke

et al. 2017

Adv Materials Inter

291

227

View full text Add to dashboard Cite

Almost ten years ago, Lee et al. [13] formulated a universal adhesive coating based on observation of the strong attachment by mussels through their byssal threads to virtually all types of inorganic and organic surfaces. The amino acid compositions of the mussel foot proteins that generate the attachment are rich in catechol and amine groups. [13][14][15] These groups associate under marine conditions, forming strong covalent and noncovalent interactions with substrates. [13] This led to the idea that both catechol and amine groups were crucial in forming robust, wide spectrum adhesive nanolayers [16,17] and consequently dopamine was conceived as a powerful building block for spontaneous deposition of thin polymer films. The dopamine monomer is deposited onto unadulterated surfaces through self-assembly and oxidative cross-linking from mild pH aqueous solution in a rapid reaction. This results in a durable, nanoscale, conformal, and hydrophilic coating that can be readily modified to introduce specific surface chemistries for a particular application. [18][19][20][21][22] These bioinspired, polydopamine materials are chemically and structurally indistinguishable from eumelanins found in the human body, [23,24] providing excellent biocompatibility. Additionally, polydopamine has broad electromagnetic absorption and excellent photothermal energy conversion [25] as well as having ionic-electronic hybrid conductivity. [26] Along with the excellent coating performance, these properties provide a vast array of potential applications for polydopamine materials.In this article, we explain what is known about polydopamine and related catecholamine coatings; their formation, the adhesion mechanism, and their physicochemical properties and we also review the applications of these materials (Figure 1). Since 2011, there have been a number of reviews on this subject matter, including general reviews on the physicochemical properties of polydopamine coatings [11,20,[27][28][29] and their applications. [20,27,30] There are also a number of more specific reviews on the chemical synthesis and modulation of polycatecholamine coatings, [31] the biomedical applications of these coatings, [8,[32][33][34] their electronic properties, [26,35] the use of polydopamine to make films at fluid interfaces, [36] in hierarchically constructed particles, [33] and capsules, [30] exploitation of polycatecholamine coatings in membrane filtration, [37] polydopamine-derived carbon materials, [38] and the use of coordination chemistry in catechol-based materials. [39] Nonetheless, this area of research is growing so rapidly [25,27] that many recent Polydopamine and related polycatecholamines can be easily deposited onto almost any surface from mild, aqueous solution. This results in durable, nanoscale coatings that exhibit high biocompatibility and have useful chemical and electronic properties. Additionally, these materials can be readily chemically and physically modified, and consequently, they are used extensively for surface modification. This ...

show abstract

Enhancement of growth and osteogenic differentiation of MC3T3-E1 cells via facile surface functionalization of polylactide membrane with chitooligosaccharide based on polydopamine adhesive coating

Cited by 21 publications

References 35 publications

Biodegradable Chitosan-graft-Poly(l-lactide) Copolymers For Bone Tissue Engineering

Biodegradable Chitosan-graft-Poly(l-lactide) Copolymers For Bone Tissue Engineering

Enhanced cell affinity and osteogenic differentiation of liquid crystal‐based substrate via surface bio‐functionalization

Versatile Surface Modification Using Polydopamine and Related Polycatecholamines: Chemistry, Structure, and Applications

Contact Info

Product

Resources

About