Orthotopic transplantation of a tissue engineered diaphragm in rats

Gubareva, Elena A.; Sjöqvist, Sebastian; Гилевич, И. В.; Сотниченко, А. С.; Куевда, Е. В.; Lim, Mei Ling; Feliu, Neus; Lemon, Greg; Даниленко, К А; Накохов, Р. З.; Gumenyuk, I. S.; Grigoriev, T. E.; Krasheninnikov, Sergey V.; Похотько, А. Г.; Басов, А. А.; Джимак, С. С.; Gustafsson, Ylva; Bautista, Geoanna; Rodríguez, Antonio Beltrán; Pokrovsky, V. M.; Jungebluth, Philipp; Чвалун, С. Н.; Holterman, Mark J.; Taylor, Doris A.; Macchiarini, Paolo

doi:10.1016/j.biomaterials.2015.11.020

Cited by 39 publications

(54 citation statements)

References 34 publications

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“…Importantly, these decellularized tissues show promise for therapeutic tissue regeneration in a variety of applications. [1-7] Development of decellularized native tissues led to the production of tissue-engineered scaffolds which retained basement membrane proteins such as collagen type IV, laminin, and fibronectin that enhance cellular adhesion [8, 9] and invoke signaling to influence cellular differentiation and regenerative potential [10-12]. Growth factors including transforming growth factor-beta, basic fibroblast growth factor (FGF), hepatocyte growth factor and vascular endothelial growth factor (VEGF) persist in their bioactive form within ECM bioscaffolds after sterilization.…”

Section: Introductionmentioning

confidence: 99%

Perivascular extracellular matrix hydrogels mimic native matrix microarchitecture and promote angiogenesis via basic fibroblast growth factor

et al. 2017

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Extracellular matrix (ECM)-derived bioscaffolds have been shown to elicit tissue repair through retention of bioactive signals. Given that the adventitia of large blood vessels is a richly vascularized microenvironment, we hypothesized that perivascular ECM contains bioactive signals that influence cells of blood vessel lineages. ECM bioscaffolds were derived from decellularized human and porcine aortic adventitia (hAdv and pAdv, respectively) and then shown have minimal DNA content and retain elastin and collagen proteins. Hydrogel formulations of hAdv and pAdv ECM bioscaffolds exhibited gelation kinetics similar to ECM hydrogels derived from porcine small intestinal submucosa (pSIS). hAdv and pAdv ECM hydrogels displayed thinner, less undulated, and fibrous microarchitecture reminiscent of native adventitia, with slight differences in ultrastructure visible in comparison to pSIS ECM hydrogels. Pepsin-digested pAdv and pSIS ECM bioscaffolds increased proliferation of human adventitia-derived endothelial cells and this effect was mediated in part by basic fibroblast growth factor (FGF2). Human endothelial cells cultured on Matrigel substrates formed more numerous and longer tube-like structures when supplemented with pAdv ECM bioscaffolds, and FGF2 mediated this matrix signaling. ECM bioscaffolds derived from pAdv promoted FGF2-dependent in vivo angiogenesis in the chick chorioallantoic membrane model. Using an angiogenesis-focused protein array, we detected 55 angiogenesis-related proteins, including FGF2 in hAdv, pAdv and pSIS ECMs. Interestingly, 19 of these factors were less abundant in ECMs bioscaffolds derived from aneurysmal specimens of human aorta when compared with nonaneurysmal (normal) specimens. This study reveals that Adv ECM hydrogels recapitulate matrix fiber microarchitecture of native adventitia, and retain angiogenesis-related actors and bioactive properties such as FGF2 signaling capable of influencing processes important for angiogenesis. This work supports the use of Adv ECM bioscaffolds for both discovery biology and potential translation towards microvascular regeneration in clinical applications.

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Section: Introductionmentioning

confidence: 99%

Perivascular extracellular matrix hydrogels mimic native matrix microarchitecture and promote angiogenesis via basic fibroblast growth factor

et al. 2017

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“…Diaphragm harvesting was performed according to the previously described protocol . Briefly, the diaphragm was dissected from the surrounding adipose tissue, then cannulated and fixed in a customized bioreactor, allowing a combined decellularization, both perfusion and agitation.…”

Section: Methodsmentioning

confidence: 99%

Impulse acoustic microscopy: A new approach for investigation of polymer and natural scaffolds

Khramtsova

Morokov

Луканина

et al. 2017

Polymer Engineering & Sci

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The aim of this article is to verify the applicability of impulse acoustic microscopy as a tool for evaluating scaffolds in tissue engineering. Decellularized rat diaphragms and nonwoven polymer scaffolds made from cellulose diacetate, chlorinated polyvinyl chloride, and polysulfone were used as model objects. Optical microscopy, scanning electron microscopy, and histological research were used as reference methods in order to realize feasibility of the acoustic microscopy method in a regenerative medicine field. Direct comparison of different methods was carried out. The high frequency ultrasound microscopy is defined as a useful non‐invasive tool for studying and investigating the bulk morphology and bulk structure of opaque scaffolds, which allows to diagnose and to assume heterogeneous regions and defects inside the specimens. POLYM. ENG. SCI., 57:709–715, 2017. © 2017 Society of Plastics Engineers

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“…The concentration of deuterium in the produced water was determined by means of a JEOL JNM-ECA 400MHz pulsed nuclear magnetic resonance spectrometer [23] . To determine the isotope D/H composition, liver samples (3.0 ± 0.1 mg each) were preliminarily freeze-dried using a lyophilizer ("LS-1000"; Prointech, Russian Federation) [24] . To determine the isotope D/H composition of the rats' liver tissues and blood plasma, a DELTA plus mass spectrometer equipped with a peripheral H/Device (Finnigan, Germany) was used for sample preparation of liquid for isotope analysis of hydrogen.…”

Section: Ddw Was Produced On a Plant Designed In Kubanmentioning

confidence: 99%

Influence of Deuterium-Depleted Water on the Isotope D/H Composition of Liver Tissue and Morphological Development of Rats at Different Periods of Ontogenesis

Басов

Anatolyevna

Alexandrovich

et al. 2019

ibj

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Background: This study aimed to evaluate the reaction of organism of laboratory animals on deuterium-depleted drinking diet. To assess the cell energy metabolism, the effect of a liquid medium with different deuterium contents on isolated liver mitochondria of random bred rats and Wistar rats was studied. Methods: This experimental study on the effect of deuterium-depleted drinking water (DDW) on 16-week-old male Wistar rats lasted for four weeks. Energy metabolism of mitochondria was examined through the production of hydrogen peroxide using an Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit. Results: Modification of isotope (deuterium-protium [D/H]) composition of rats' blood and organ tissues with DDW (-705‰), introduced into rats' diet within four weeks, led to the formation of isotope D/H gradient between blood plasma and organ tissues and affected isotope D/H exchange reactions on the adaptive processes. The isolated liver mitochondria from the random bred rats consumed DDW presented a maximum increase in H 2 O 2 production during the incubation in DDW medium. This increased level of H 2 O 2 production was higher in the isolated liver mitochondria of the rats consuming natural deuterium content drinking water (-24‰). Conclusion: The obtained results indicate the possibility of nutritional correction of isotope D/H metabolism in blood by means of products with modified isotope composition, as well as the prospects of using isotope exchange reactions in case of imbalance in function of the body's defense systems in different generations of animals.

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Orthotopic transplantation of a tissue engineered diaphragm in rats

Cited by 39 publications

References 34 publications

Perivascular extracellular matrix hydrogels mimic native matrix microarchitecture and promote angiogenesis via basic fibroblast growth factor

Perivascular extracellular matrix hydrogels mimic native matrix microarchitecture and promote angiogenesis via basic fibroblast growth factor

Impulse acoustic microscopy: A new approach for investigation of polymer and natural scaffolds

Influence of Deuterium-Depleted Water on the Isotope D/H Composition of Liver Tissue and Morphological Development of Rats at Different Periods of Ontogenesis

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