Development of artificial tissues or organs is one of the actual tasks in regenerative medicine that requires observation and evaluation of intact volume microstructure of tissue engineering products at all stages of their formation, from native donor tissues and decellularized scaffolds to recipient cell migration in the matrix. Unfortunately in practice, methods of vital noninvasive imaging of volume microstructure in matrixes are absent. In this work, we propose a new approach based on high‐frequency acoustic microscopy for noninvasive evaluation and visualization of volume microstructure in tissue engineering products. The results present the ultrasound characterization of native rat diaphragms and lungs and their decellularized scaffolds. Verification of the method for visualization of tissue formation in the matrix volume was described in the model samples of diaphragm scaffolds with stepwise collagenization. Results demonstrate acoustic microscopic sensitivity to cell content concentration, variation in local density, and orientation of protein fibers in the volume, micron air inclusions, and other inhomogeneities of matrixes.

No abstract

No abstract

Atomic hydrogen interaction with the Si͑111͒4ϫ1-In surface phase was studied using low-energy electron diffraction, Auger electron spectroscopy, and scanning tunneling microscopy. Upon hydrogen action mostly Si-In outer bonds are broken and are replaced by Si-H, and In is freed to form islands without Si movement. It was found that the underlying atomic layer of a substrate of the Si͑111͒4ϫ1-In surface phase has a reconstruction with the same periodicity as the In layer. A structural model of this substrate reconstruction is proposed based on the recently proposed extended Pandey chain model for the Si͑111͒3ϫ1 Ag-and alkalimetals-induced substrate reconstruction.

No abstract

The aim of this article is to verify the applicability of impulse acoustic microscopy as a tool for evaluating scaffolds in tissue engineering. Decellularized rat diaphragms and nonwoven polymer scaffolds made from cellulose diacetate, chlorinated polyvinyl chloride, and polysulfone were used as model objects. Optical microscopy, scanning electron microscopy, and histological research were used as reference methods in order to realize feasibility of the acoustic microscopy method in a regenerative medicine field. Direct comparison of different methods was carried out. The high frequency ultrasound microscopy is defined as a useful non‐invasive tool for studying and investigating the bulk morphology and bulk structure of opaque scaffolds, which allows to diagnose and to assume heterogeneous regions and defects inside the specimens. POLYM. ENG. SCI., 57:709–715, 2017. © 2017 Society of Plastics Engineers

No abstract

No abstract