Influence of Deuterium-Depleted Water on the Isotope D/H Composition of Liver Tissue and Morphological Development of Rats at Different Periods of Ontogenesis

Басов, А. А.; Anatolyevna, Elkina Аnna; Alexandrovich, Samkov Andrei; Николаевич, Волченко Никита; Victorovich, Moiseev Arcadii; Fedulova, Liliya; Gennadievich, Baryshev Mikhail; Сергеевич, Джимак Степан

doi:10.29252/ibj.23.2.129

Cited by 16 publications

(1 citation statement)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The administration of water with a reduced deuterium content into the drinking diet leads to a change in the 2 H/ 1 H ratio in the body tissues due to the hydrogen isotopes exchange [18], in particular, as shown by the recent study, the antiproliferation effects of DDW are associated with the occurrence of an imbalance in the mitochondria between the production of reactive oxygen species and their neutralization [19]. Some studies have shown an increase in H 2 O 2 production by mitochondria during their pre-incubation in the deuterium depleted media [20,21]. In addition, the mechanisms of DDW influence on biological systems from the point of view of the medical biochemistry are logically explained by a modification in the mitochondria energy balance [22,23].…”

Section: Introductionmentioning

confidence: 95%

Inequality in the Frequency of the Open States Occurrence Depends on Single 2H/1H Replacement in DNA

et al. 2020

View full text Add to dashboard Cite

In the present study, the effect of 2H/1H isotopic exchange in hydrogen bonds between nitrogenous base pairs on occurrence and open states zones dynamics is investigated. These processes are studied using mathematical modeling, taking into account the number of open states between base pairs. The calculations of the probability of occurrence of open states in different parts of the gene were done depending on the localization of the deuterium atom. The mathematical modeling study demonstrated significant inequality (dependent on single 2H/1H replacement in DNA) among three parts of the gene similar in length of the frequency of occurrence of the open states. In this paper, the new convenient approach of the analysis of the abnormal frequency of open states in different parts of the gene encoding interferon alpha 17 was presented, which took into account both rising and decreasing of them that allowed to make a prediction of the functional instability of the specific DNA regions. One advantage of the new algorithm is diminishing the number of both false positive and false negative results in data filtered by this approach compared to the pure fractile methods, such as deciles or quartiles.

show abstract

Section: Introductionmentioning

confidence: 95%

Inequality in the Frequency of the Open States Occurrence Depends on Single 2H/1H Replacement in DNA

et al. 2020

View full text Add to dashboard Cite

show abstract

Short term deuterium depletion in drinking water reduced tumor induced oxidative stress in mice liver

Bayrak¹,

Kulak²,

Yanardağ³

et al. 2022

Pathology - Research and Practice

View full text Add to dashboard Cite

Effect of the deuterium on efficiency and type of adipogenic differentiation of human adipose-derived stem cells in vitro

Zlatska

Vasyliev

Gordiienko

et al. 2020

Sci Rep

View full text Add to dashboard Cite

In this study, we performed an adipogenic differentiation of human adipose-derived stem cells (ADSCs) in vitro with different deuterium content (natural, low and high) in the culture medium during differentiation process with parallel analysis of the gene expression, metabolic activity and cell viability/ toxicity. After ADSCs differentiation into adipocytes we have done the analysis of differentiation process efficiency and determined a type of resulting adipocytes (by morphology, gene expression, UCP1 protein detection and adipokine production analysis). We have found that high (5 × 10 5 ppm) deuterium content significantly inhibit in vitro adipogenic differentiation of human ADSCs compared to the groups with natural (150 ppm) and low (30 ppm) deuterium content. Importantly, protocol of differentiation used in our study leads to white adipocytes development in groups with natural (control) and high deuterium content, whereas deuterium-depleted differentiation medium leads to brown-like (beige) adipocytes formation. We have also remarked the direct impact of deuterium on the cellular survival and metabolic activity. Interesting, in deuterium depleted-medium, the cells had normal survival rate and high metabolic activity, whereas the inhibitory effect of deuterated medium on ADSCs differentiation at least was partly associated with deuterium cytotoxicity and inhibitory effect on metabolic activity. The inhibitory effect of deuterium on metabolic activity and the subsequent decrease in the effectiveness of adipogenic differentiation is probably associated with mitochondrial dysfunction. Thus, deuterium could be considered as an element that affects the substance chirality. These findings may be the basis for the development of new approaches in the treatment of obesity, metabolic syndrome and diabetes through the regulation of adipose-derived stem cell differentiation and adipocyte functions. In the 21st century, non-communicable diseases (NCD) like obesity, metabolic syndrome and type 2 diabetes mellitus (T2DM) became the main medical problems of the humanity 1-4. These diseases started in the Western world, but in parallel with the improving of human life standards, technological progress and the spread of the Western lifestyle also around the world. So, these diseases have become a global epidemic 5. Currently, although there remains a correlation between the level of economic development and the frequency of these diseases, they have ceased to be a medical problem in high-income countries, but also have become an urgent item for the low-income and middle-income countries 6. A characteristic feature of obesity, metabolic syndrome and T2DM is the defection of glucose and lipids metabolism, which is manifested in insulin resistance, impaired fasting glucose, dyslipidemia, high blood sugar, high serum triglycerides, imbalance of different types of lipoproteins in blood serum 7,8 .

show abstract

Influence of Deuterium-Depleted Water on the Isotope D/H Composition of Liver Tissue and Morphological Development of Rats at Different Periods of Ontogenesis

Cited by 16 publications

References 37 publications

Inequality in the Frequency of the Open States Occurrence Depends on Single 2H/1H Replacement in DNA

Inequality in the Frequency of the Open States Occurrence Depends on Single 2H/1H Replacement in DNA

Short term deuterium depletion in drinking water reduced tumor induced oxidative stress in mice liver

Effect of the deuterium on efficiency and type of adipogenic differentiation of human adipose-derived stem cells in vitro

Contact Info

Product

Resources

About