Inequality in the Frequency of the Open States Occurrence Depends on Single 2H/1H Replacement in DNA

Басов, А. А.; Drobotenko, M. I.; Svidlov, Alexander; Gerasimenko, E. O.; Малышко, В. В.; Елкина, А. А.; Барышев, М. Г.; Джимак, С. С.

doi:10.3390/molecules25163753

Cited by 18 publications

(17 citation statements)

References 62 publications

(93 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Perhaps, in our opinion, the key mechanism is the chirality of substances in methylene groups with deuterium or protium in their structure. Recent data with a single 2 H/ 1 H replacement in DNA confirm this mechanism [65]. Therefore, further experimental data will help to solve the mechanism of action of deuterated or deuterium-depleted aqueous media on living organisms.…”

Section: Kinetic Model Of the Vital Activity Of Living Organisms Depending On The D/h Ratiomentioning

confidence: 84%

Dependence of Biocatalysis on D/H Ratio: Possible Fundamental Differences for High-Level Biological Taxons

et al. 2020

View full text Add to dashboard Cite

The kinetics of biological reactions depends on the deuterium/protium (D/H) ratio in water. In this work, we describe the kinetic model of biocatalytic reactions in living organisms depending on the D/H ratio. We show that a change in the lifetime or other characteristics of the vital activity of some organisms in response to a decrease or increase in the content of deuterium in the environment can be a sign of a difference in taxons. For animals—this is a curve with saturation according to the Gauss’s principle, for plants—it is the Poisson dependence, for bacteria a weakly saturated curve with a slight reaction to the deuterium/protium ratio toward increasing deuterium. The biological activity of the aquatic environment with reduced, elevated, and natural concentrations of deuterium is considered. The results of the study are presented in different vital indicators of some taxons: the bacteria kingdom—the colony forming units (CFU) index (Escherichia coli); animals—the activation energy of the death of ciliates (Spirostomum ambiguum), embryogenesis of fish (Brachydanio rerio); plants—germination and accumulation of trace elements Callisia fragrans L., sprouting of gametophores and peptidomics of moss Physcomitrella patens. It was found that many organisms change their metabolism and activity, responding to both high and low concentrations of deuterium in water.

show abstract

Section: Kinetic Model Of the Vital Activity Of Living Organisms Depending On The D/h Ratiomentioning

confidence: 84%

Dependence of Biocatalysis on D/H Ratio: Possible Fundamental Differences for High-Level Biological Taxons

et al. 2020

View full text Add to dashboard Cite

show abstract

“…In our previous study for

N·m energy range [ 29 ], the inequality in the frequency of the open states occurrence because of the single 2 H/ 1 H replacement in gene encoding interferon alpha 17 (IFNA17, which consists of 980 nucleotide pairs [ 30 ]) was proved through it conditionally divided into three equal parts: from the 1st to the 327th nucleobases (I part), from the 328th to the 653th nucleobases (II part) and from the 654th to the 980th nucleobases (III part). This approach allowed us to contemplate the influence on probabilities (

) of open state occurrences between different nitrogenous bases in double-stranded DNA dependent on the single 2 H/ 1 H replacement in basepair of each gene region.…”

Section: Resultsmentioning

confidence: 99%

Influence of Single Deuterium Replacement on Frequency of Hydrogen Bond Dissociation in IFNA17 under the Highest Critical Energy Range

Басов

Drobotenko

Svidlov

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

The effect of single substitutions of protium for deuterium in hydrogen bonds between pairs of nitrogenous bases on the open states occurrence probability at high critical breaking energies of these bonds has been studied. The study was carried out using numerical methods based on the angular mathematical model of DNA. The IFNA17 gene was divided into three approximately equal parts. A comparison of the open states occurrence probability in these parts of the gene was done. To improve the accuracy of the results, a special data processing algorithm was developed. The developed methods have shown their suitability for taking into account the occurrence of open states in the entire range of high critical energies. It has been established that single 2H/1H substitutions in certain nitrogenous bases can be a mechanism for maintaining the vital activity of IFNA17 under critical conditions. In general, the developed method of the mathematical modeling provide unprecedented insight into the DNA behavior under the highest critical energy range, which greatly expands scientific understanding of nucleobases interaction.

show abstract

“…At the same time, even relatively small increases or decreases in its concentration affect physiological processes at all levels: molecular, cellular, tissue, organ and organism [ 6 , 7 , 8 , 9 , 10 ]. Many studies have shown that a decrease in the deuterium content supports the body’s defense systems, including nonspecific mechanisms [ 11 , 12 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Reduction of Deuterium Level Supports Resistance of Neurons to Glucose Deprivation and Hypoxia: Study in Cultures of Neurons and on Animals

et al. 2021

Self Cite

View full text Add to dashboard Cite

The effect of a reduced deuterium (D) content in the incubation medium on the survival of cultured neurons in vitro and under glucose deprivation was studied. In addition, we studied the effect of a decrease in the deuterium content in the rat brain on oxidative processes in the nervous tissue, its antioxidant protection, and training of rats in the T-shaped maze test under hypoxic conditions. For experiments with cultures of neurons, 7–8-day cultures of cerebellar neurons were used. Determination of the rate of neuronal death in cultures was carried out using propidium iodide. Acute hypoxia with hypercapnia was simulated in rats by placing them in sealed vessels with a capacity of 1 L. The effect on oxidative processes in brain tissues was assessed by changes in the level of free radical oxidation and malondialdehyde. The effect on the antioxidant system of the brain was assessed by the activity of catalase. The study in the T-maze was carried out in accordance with the generally accepted methodology, the skill of alternating right-sided and left-sided loops on positive reinforcement was developed. This work has shown that a decrease in the deuterium content in the incubation medium to a level of −357‰ has a neuroprotective effect, increasing the survival rate of cultured neurons under glucose deprivation. When exposed to hypoxia, a preliminary decrease in the deuterium content in the rat brain to −261‰ prevents the development of oxidative stress in their nervous tissue and preserves the learning ability of animals in the T-shaped maze test at the level of the control group. A similar protective effect during the modification of the 2H/1H internal environment of the body by the consumption of DDW can potentially be used for the prevention of pathological conditions associated with the development of oxidative stress with damage to the central nervous system.

show abstract

Inequality in the Frequency of the Open States Occurrence Depends on Single 2H/1H Replacement in DNA

Cited by 18 publications

References 62 publications

Dependence of Biocatalysis on D/H Ratio: Possible Fundamental Differences for High-Level Biological Taxons

Dependence of Biocatalysis on D/H Ratio: Possible Fundamental Differences for High-Level Biological Taxons

Influence of Single Deuterium Replacement on Frequency of Hydrogen Bond Dissociation in IFNA17 under the Highest Critical Energy Range

Reduction of Deuterium Level Supports Resistance of Neurons to Glucose Deprivation and Hypoxia: Study in Cultures of Neurons and on Animals

Contact Info

Product

Resources

About