In order to understand the electrical conduction mechanisms in the transfer characteristics of pentacene-based organic thin film transistors (OTFTs), an analysis using the temperature-dependent transfer characteristics is presented. The temperature-dependent transfer characteristics exhibit hopping conduction behavior. Compared to the fitting data for the temperature-dependent linear-regime (saturation-regime) transfer characteristics of OTFTs, the fitting data for the temperature-dependent sub-threshold-regime transfer characteristics of OTFTs show that a longer hopping distance and a higher barrier height for hopping result in a higher channel resistance. However, similar hopping conduction behavior is seen in the saturation and linear regions, which demonstrates that the carrier mobility is drain-source voltage-dependent.