Organ regeneration: integration application of cell encapsulation and 3D bioprinting

Liu, Huanbao; Zhou, Huixing; Lan, Haiming; Li, Tianyu

doi:10.1080/17452759.2017.1338065

Cited by 17 publications

(9 citation statements)

References 87 publications

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“… Optimized nozzle structure of 3D bioprinter. 1—Quick-connect ( a ), 2—Quick-connect ( b ), 3—Cone holder, 4—Nozzle body, 5—Set Nut, 6—pinhead, 7—Sealing screw, and 8—Capillary [ 26 , 27 ]. …”

Section: Figurementioning

confidence: 99%

“…Therefore, three-dimensional axisymmetric flow-focusing was applied by GAO Q. et al [ 25 ] to fabricate vessel-like structures, which proved that flow-focusing method can improve the cell survival rate. Meanwhile, Liu, H. et al [ 26 , 27 ] have adopted the flow-focusing method to obtain vessel-like structures and analyzed the synchronization among nozzle extrusion, nozzle speed, and rotating speed based on an extrusion-based 3D bioprinter, which could improve the modeling effect in this forming method. As mentioned before, it can be seen that the flow-focusing method can achieve better formation of tissue and organ structure.…”

Section: Introductionmentioning

confidence: 99%

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Simulation Analysis of the Influence of Nozzle Structure Parameters on Material Controllability

et al. 2020

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With the evolution of three-dimensional (3D) printing, many restrictive factors of 3D printing have been explored to upgrade the feasibility of 3D printing technology, such as nozzle structure, print resolution, cell viability, etc., which has attracted extensive attention due to its possibility of curing disease in tissue engineering and organ regeneration. In this paper, we have developed a novel nozzle for 3D printing, numerical simulation, and finite element analysis have been used to optimize the nozzle structure and further clarified the influence of nozzle structure parameters on material controllability. Using novel nozzle structure, we firstly adopt ANSYS-FLUENT to analyze material controllability under the different inner cavity diameter, outer cavity diameter and lead length. Secondly, the orthogonal experiments with the novel nozzle are carried out in order to verify the influence law of inner cavity diameter, outer cavity diameter, and lead length under all sorts of conditions. The experiment results show that the material P diameter can be controlled by changing the parameters. The influence degree of parameters on material P diameter is shown that lead length > inner cavity diameter > outer cavity diameter. Finally, the optimized parameters of nozzle structure have been adjusted to estimate the material P diameter in 3D printing.

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Section: Figurementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Simulation Analysis of the Influence of Nozzle Structure Parameters on Material Controllability

et al. 2020

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“…According to relevant reports [1], the organ donation rate in China has reached 2/million, and the shortage of organ transplant donors is serious. With the development of 3D bio-printing technology, in vitro tissue or organ reconstruction has become an effective means to solve the shortage of donors, and is also an important direction in the field of tissue engineering [2]. In recent years, important achievements have been made in the regeneration of blood vessels, cartilage, heart, and other organs.…”

Section: Introductionmentioning

confidence: 99%

Theoretical and Experimental Research on Multi-Layer Vessel-like Structure Printing Based on 3D Bio-Printing Technology

et al. 2021

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Cardiovascular disease is the leading cause of death worldwide. Traditional autologous transplantation has become a severe issue due to insufficient donors. Artificial blood vessel is an effective method for the treatment of major vascular diseases, such as heart and peripheral blood vessel diseases. However, the traditional single-material printing technology has been unable to meet the users’ demand for product functional complexity, which is not only reflected in the field of industrial manufacturing, but also in the field of functional vessel-like structure regeneration. In order to achieve the printing and forming of multi-layer vessel-like structures, this paper carries out theoretical and experimental research on the printing and forming of a multi-layer vessel-like structure based on multi-material 3D bioprinting technology. Firstly, theoretical analysis has been explored to research the relationship among the different parameters in the process of vessel forming, and further confirm the synchronous relationship among the extrusion rate of material, the tangential speed of the rotating rod, and the movement speed of the platform. Secondly, sodium alginate and gelatin have been used as the experimental materials to manufacture the vessel-like structure, and the corrected parameter of the theoretical analysis is further verified. Finally, the cell-loaded materials have been printed and analyzed, and cell viability is more than 90%, which provides support for the research of multi-layer vessel-like structure printing.

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“…3D printing is a novel technology to be applied to regenerative medicine that has attracted extensive attention due to its potential to cure disease through tissue engineering and organ regeneration [7,8,9,10,11,12]. In the past few years, many functional tissues and organs were manufactured by 3D printing systems such as inkjet-based printing [13,14,15], micro-extrusion printing [16], and laser-assisted printing [17,18,19], which have different forming parameters in different 3D printing system.…”

Section: Introductionmentioning

confidence: 99%

3D Printing of Artificial Blood Vessel: Study on Multi-Parameter Optimization Design for Vascular Molding Effect in Alginate and Gelatin

et al. 2017

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3D printing has emerged as one of the modern tissue engineering techniques that could potentially form scaffolds (with or without cells), which is useful in treating cardiovascular diseases. This technology has attracted extensive attention due to its possibility of curing disease in tissue engineering and organ regeneration. In this paper, we have developed a novel rotary forming device, prepared an alginate–gelatin solution for the fabrication of vessel-like structures, and further proposed a theoretical model to analyze the parameters of motion synchronization. Using this rotary forming device, we firstly establish a theoretical model to analyze the thickness under the different nozzle extrusion speeds, nozzle speeds, and servo motor speeds. Secondly, the experiments with alginate–gelatin solution are carried out to construct the vessel-like structures under all sorts of conditions. The experiment results show that the thickness cannot be adequately predicted by the theoretical model and the thickness can be controlled by changing the parameters. Finally, the optimized parameters of thickness have been adjusted to estimate the real thickness in 3D printing.

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Organ regeneration: integration application of cell encapsulation and 3D bioprinting

Cited by 17 publications

References 87 publications

Simulation Analysis of the Influence of Nozzle Structure Parameters on Material Controllability

Simulation Analysis of the Influence of Nozzle Structure Parameters on Material Controllability

Theoretical and Experimental Research on Multi-Layer Vessel-like Structure Printing Based on 3D Bio-Printing Technology

3D Printing of Artificial Blood Vessel: Study on Multi-Parameter Optimization Design for Vascular Molding Effect in Alginate and Gelatin

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