Oral inoculation of probioticsLactobacillus acidophilusNCFM suppresses tumour growth both in segmental orthotopic colon cancer and extra-intestinal tissue

Abstract

Modulation of the cellular response by the administration of probiotic bacteria may be an effective strategy for preventing or inhibiting tumour growth. We orally pre-inoculated mice with probiotics Lactobacillus acidophilus NCFM (La) for 14 d. Subcutaneous dorsal-flank tumours and segmental orthotopic colon cancers were implanted into mice using CT-26 murine colon adenocarcinoma cells. On day 28 after tumour initiation, the lamina propria of the colon, mesenteric lymph nodes (MLN) and spleen were harvested and purified for flow cytometry and mRNA analyses. We demonstrated that La pre-inoculation reduced tumour volume growth by 50·3 %, compared with untreated mice at 28 d after tumour implants (2465·5 (SEM 1290·4) v. 4950·9 (SEM 1689·3) mm 3 , P, 0·001). Inoculation with La reduced the severity of colonic carcinogenesis caused by CT-26 cells, such as level of colonic involvement and structural abnormality of epithelial/ crypt damage. Moreover, La enhanced apoptosis of CT-26 cells both in dorsal-flank tumour and segmental orthotopic colon cancer, and the mean counts of apoptotic body were higher in mice pre-inoculated with La (P,0·05) compared with untreated mice. La pre-inoculation down-regulated the CXCR4 mRNA expressions in the colon, MLN and extra-intestinal tissue, compared with untreated mice (P,0·05). In addition, La pre-inoculation reduced the mean fluorescence index of MHC class I (H-2Dd, -Kd and -Ld) in flow cytometry analysis. Taken together, these findings suggest that probiotics La may play a role in attenuating tumour growth during CT-26 cell carcinogenesis. The down-regulated expression of CXCR4 mRNA and MHC class I, as well as increasing apoptosis in tumour tissue, indicated that La may be associated with modulating the cellular response triggered by colon carcinogenesis. Key words: Probiotics: Lactobacillus acidophilus NCFM: CT-26 cells: Colon carcinogenesis: ApoptosisColon carcinoma is a leading cause of digestive system neoplasm. The colon cancer mortality rate is second only to that of lung cancer in men and breast cancer in women, and colon cancer rates have increased over the past 20-30 years (1) . Diet contributes to colon cancer risk. In fact, up to 75 % of cases are thought to be associated with diet (2) , indicating that a person can reduce his or her colon cancer risk simply via diet modification.Probiotics consist of a preparation of viable micro-organisms that alter the existing microflora of the intestine, thereby

Lactobacillus Salivarius REN Inhibits Rat Oral Cancer Induced by 4-Nitroquioline 1-Oxide

“…It has been found that some probiotics could also retard the growth of tumor volume and enhance the apoptosis of tumor cells, but studies were mainly focused on colon tumors (41)(42)(43). Our results (Table 3) showed a significant antiproliferation and proapoptotic effect that correlates with suppression of 4NQO-induced oral cancer incidence by dietary L. salivarius REN are in line with these observations.…”

Probiotics as a preventive strategy for surgical infection in colorectal cancer patients: a systematic review and meta-analysis of randomized trials

“…For this purpose, the present study evaluated the prophylactic and therapeutic effects of probiotics on patients with CRC in the pre-and postoperative, and at the same time searched for preventive strategies from recent experimental models. A large number of studies have shown that probiotics can prevent the initiation or development of cancer by changing the intestinal microbial composition, host protection against pathogenic bacteria and fungi, the production of biological substances such as short-chain fatty acid and conjugated linoleic acid, inactivation of carcinogenic compounds, improvement of gut barrier function, modulation of immune responses, apoptosis, anti-proliferative effects and antioxidant activity (21)(22)(23) (18) observed better outcomes by using these microorganisms in the therapeutic intervention for patients with CRC. Perioperative administration of probiotics reduces the inflammatory response, which can lead to a reduction in length of hospital stay.…”

Viability of Lactobacillus acidophilus NCFM Howaru Dophilus during storage at refrigeration temperatures

“…Moreover, consumption of probiotic Lb. acidophilus NCFM resulted in the retardation of the growth of tumor volume and the enhancement of the apoptosis of tumor cells (Chen et al, 2012). The cells of this strain are reported to adhere to the HT-29 and Caco-2 cells derived from colon carcinoma (Goh and Klaenhammer, 2010).…”