Optimization of succinic acid fermentation with Actinobacillus succinogenes by response surface methodology (RSM)

Zhang, Yunjian; Li, Qiang; Zhang, Yuxiu; Wang, Dan; Xing, Jianmin

doi:10.1631/jzus.b1100134

Cited by 28 publications

(22 citation statements)

References 20 publications

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“…The closer the R 2 to 1.0, the stronger the model and the better it predicts the response 31 . The adjusted R 2 , which corrects the R 2 value for the sample size and the number of terms, was 0.7739 32 .…”

Section: Resultsmentioning

confidence: 99%

Enhanced polyhydroxybutyrate (PHB) production by newly isolated rare actinomycetes Rhodococcus sp. strain BSRT1-1 using response surface methodology

Trakunjae

Boondaeng

Apiwatanapiwat

et al. 2021

Sci Rep

101

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Poly-β-hydroxybutyrate (PHB) is a biodegradable polymer, synthesized as carbon and energy reserve by bacteria and archaea. To the best of our knowledge, this is the first report on PHB production by a rare actinomycete species, Rhodococcus pyridinivorans BSRT1-1. Response surface methodology (RSM) employing central composite design, was applied to enhance PHB production in a flask scale. A maximum yield of 3.6 ± 0.5 g/L in biomass and 43.1 ± 0.5 wt% of dry cell weight (DCW) of PHB were obtained when using RSM optimized medium, which was improved the production of biomass and PHB content by 2.5 and 2.3-fold, respectively. The optimized medium was applied to upscale PHB production in a 10 L stirred-tank bioreactor, maximum biomass of 5.2 ± 0.5 g/L, and PHB content of 46.8 ± 2 wt% DCW were achieved. Furthermore, the FTIR and 1H NMR results confirmed the polymer as PHB. DSC and TGA analysis results revealed the melting, glass transition, and thermal decomposition temperature of 171.8, 4.03, and 288 °C, respectively. In conclusion, RSM can be a promising technique to improve PHB production by a newly isolated strain of R. pyridinivorans BSRT1-1 and the properties of produced PHB possessed similar properties compared to commercial PHB.

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Section: Resultsmentioning

confidence: 99%

Enhanced polyhydroxybutyrate (PHB) production by newly isolated rare actinomycetes Rhodococcus sp. strain BSRT1-1 using response surface methodology

Trakunjae

Boondaeng

Apiwatanapiwat

et al. 2021

Sci Rep

101

View full text Add to dashboard Cite

show abstract

“…The conventional method to optimize the medium is the onefactor-at-a-time method, which is time-consuming and cannot account for interactions between different parameters. Response surface methodology (RSM) is a statistical method used to describe and predict the response of a system of multiple variables with fewer experiments, therefore time and cost effective (Deepak et al, 2008;Mutalik et al, 2008;Zhang et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Enhancing production of a 24-membered ring macrolide compound by a marine bacterium using response surface methodology

Chen

Chen³

et al. 2013

J. Zhejiang Univ. Sci. B

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A 24-membered ring macrolide compound, macrolactin A has potential applications in pharmaceuticals for its anti-infectious and antiviral activity. In this study, macrolactin A was produced by a marine bacterium, which was identified as Bacillus subtilis by 16S ribosomal RNA (rRNA) sequence analysis. Electrospray ionization mass spectrometry (ESI/MS) and nuclear magnetic resonance (NMR) spectroscopy analyses were used to characterize this compound. To improve the production, response surface methodology (RSM) involving Box-Behnken design (BBD) was employed. Faeces bombycis, the main by-product in sericulture, was used as a nitrogen source in fermentation. The interactions between three significant factors, F. bombycis, soluble starch, and (NH 4 ) 2 SO 4 were investigated. A quadratic model was constructed to fit the production and the factors. Optimum medium composition was obtained by analysis of the model. When cultivated in the optimum medium, the production of macrolactin A was increased to 851 mg/L, 2.7 times as compared to the original. This study is also useful to find another way in utilizing F. bombycis.

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“…In addition, the impact of CaCl 2 turned out statistically insignificant. The key importance of MgCO 3 for the production of succinic acid by bacteria is relatively well document in different research works (Lee et al 1999 ; Zhang et al 2012 ). This compound is responsible for the control of medium pH and delivers Mg 2+ ions being indispensable for the key enzyme in the succinic acid biosynthesis pathway, namely phosphoenolpyruvate carboxykinase.…”

Section: Discussionmentioning

confidence: 95%

Media optimization for economic succinic acid production by Enterobacter sp. LU1.

et al. 2017

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Enterobacter sp. LU1 could efficiently convert glycerol to succinic acid under anaerobic conditions after the addition of lactose. In this study, media constituents affecting both Enterobacter sp. LU1 biomass and succinic acid production were investigated employing response surface methodology (RSM) with central composite design. Statistical methods led to the development of an efficient and inexpensive microbiological media based on crude glycerol, whey permeate as carbon sources and urea as a nitrogen source. The optimized production of bacterial biomass in aerobic conditions was predicted and the interactive effects between crude glycerol, urea and magnesium sulfate were investigated. As a result, a model for predicting the concentration of bacterial biocatalyst biomass was developed with crude glycerol as a sole carbon source. In addition, it was observed that the interactive effect between crude glycerol and urea was statistically significant. Response surface methodology was also employed to develop the model for predicting the concentration of succinic acid produced. Validity of the model was confirmed during verification experiments wherein actual results differed from predicted values by 0.77%. The applied statistical methods proved the feasibility for anaerobic succinic acid production on crude glycerol without expensive yeast extract addition. In conclusion, the RSM method can provide valuable information for succinic acid scale-up fermentation using Enterobacter sp. LU1.

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Optimization of succinic acid fermentation with Actinobacillus succinogenes by response surface methodology (RSM)

Cited by 28 publications

References 20 publications

Enhanced polyhydroxybutyrate (PHB) production by newly isolated rare actinomycetes Rhodococcus sp. strain BSRT1-1 using response surface methodology

Enhanced polyhydroxybutyrate (PHB) production by newly isolated rare actinomycetes Rhodococcus sp. strain BSRT1-1 using response surface methodology

Enhancing production of a 24-membered ring macrolide compound by a marine bacterium using response surface methodology

Media optimization for economic succinic acid production by Enterobacter sp. LU1.

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