Enhancing production of a 24-membered ring macrolide compound by a marine bacterium using response surface methodology

Chen, Hua; Wu, Mianbin; Chen, Zheng-Jie; Wang, Ming-lu; Lin, Johnson; Yang, Lirong

doi:10.1631/jzus.b1200153

Cited by 10 publications

(8 citation statements)

References 14 publications

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“…S3) with that of the predicted one (Predicted R 2 = 0.988). As a general rule, closer the R 2 value to 1, the model is considered stronger to predict the responses [28]. The observed R 2 value was also similar to many earlier reports [29,30].…”

Section: Discussionsupporting

confidence: 89%

Statistical optimization of lignocellulosic waste containing culture medium for enhanced production of cellulase by Bacillus tequilensis G9

Dar

Pawar

Chintalchere

et al. 2019

Waste Dispos. Sustain. Energy

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The ever increasing energy demands of modern civilization and rapidly dwindling fossil fuels point towards a renewable substitute like biofuels. However, higher costs associated with biofuel productions is the major bottleneck for its commercialization. The present study demonstrates the use of a statistical approach called response surface methodology (RSM) to investigate the optimum parameters for maximum production of cellulase by Bacillus tequilensis G9. The Plackett-Burman design (PB) of the RSM analysis indicated grass straw (GS) concentration, pH, FeSO 4 , inoculum, MgSO4, incubation period and NH 4 Cl as significant variables that influence the cellulase production. Further, to propose the best medium for the maximum production of cellulase by B. tequilensis G9, the most influential parameters, namely concentrations of GS as substrate, FeSO 4 , pH, inoculum size, etc. were fine-tuned by central composite design (CCD) involving four factors and five levels. The CCD analysis demonstrated 8% substrate concentration, 1.5% of inoculum along with 10 ppm FeSO 4 and a pH of 5.5 in media as optimum conditions for highest enzyme production. The field emission scanning electron microscopic analysis of the treated GS showed structural alterations depicting significant deconstruction caused by B. tequilensis G9. The yield of the partially purified cellulase proteins were found to be 21% revealing molecular mass between 30 and 97 kDa. The enhanced cellulase production by B. tequilensis G9 demonstrated in our study brands its applications in many industrial processes like biorefinery, biofuels, etc.

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Section: Discussionsupporting

confidence: 89%

Statistical optimization of lignocellulosic waste containing culture medium for enhanced production of cellulase by Bacillus tequilensis G9

Dar

Pawar

Chintalchere

et al. 2019

Waste Dispos. Sustain. Energy

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“…The developed model was found to be very effective in optimizing the selected medium components evident from R 2 value 0.9264. The closer R 2 is to 1, the stronger is the model to predict the response ( Chen et al, 2013 ). The observed R 2 value was comparable with the earlier reports ( Wang et al, 2011 ; Rajeswari et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Optimization of cellulase production by Enhydrobacter sp. ACCA2 and its application in biomass saccharification

et al. 2015

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Cellulase finds use in saccharification of lignocellulosic agroresidues to fermentable sugars which can be used for production of commercially important metabolites. This study reports endoglucanase (CMCase) production by Enhydrobacter sp. ACCA2. The CMCase activity of the strain ACCA2 was successively improved by optimization of range of physical and nutritional parameter in a set of non-statistical and statistical experiments. Initial non-statistical selection of carbon source, incubation time, temperature and pH resulted in 1.07 fold increase of CMCase activity. In a subsequent statistical method, response surface methodology, optimization of medium components such as carboxymethylcellulose, peptone, NaCl, MgSO4, K2HPO4, and (NH4)2SO4 yielded further increase up to 2.39 fold CMCase activity. The cellulolytic potential was evaluated in biomass saccharification with different plant materials and the results revealed that the enzyme produced by strain may have significant commercial values for industrial saccharification process. Moreover, this is the first report of cellulase production by an Enhydrobacter spp.

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“…Accuracy of the model can be checked by the determination of coefficient of R 2 . The closer the value of R 2 to 1, the stronger the model to predict the response [34]. The model R 2 of 0.9972 implied that model equation could explain 99.72% of the total variation in the response.…”

Section: Resultsmentioning

confidence: 99%

Successive Nonstatistical and Statistical Approaches for the Improved Antibiotic Activity of Rare ActinomyceteNonomuraeasp. JAJ18

Jose

Jebakumar

2014

BioMed Research International

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The selection and optimization of nutritional constituents as well as their levels for the improved production of antibiotic by Nonomuraea sp. JAJ18 were carried out using combination of both nonstatistical one-factor-at-a-time (OFAT) method and statistical response surface methodology (RSM). Using OFAT method, starch and (NH4)2SO4 were identified as suitable carbon and nitrogen sources, respectively. Subsequently, starch, NaCl, and MgSO4·7H2O were recognized as the most significant media components with confidence level of above 95% using the Plackett-Burman design. The levels of the three media components were further optimized using RSM employed with Box-Behnken design. Accordingly, a second-order polynomial regression model was fitted into the experimental data. By analyzing the response surface plots as well as using numerical optimization method, the optimal levels for starch, NaCl, and MgSO4·7H2O were determined as 15.6 g/L, 0.8 g/L, and 1.98 g/L, respectively. With the optimized medium, 15.5% increase was observed in antibiotic activity of JAJ18. Results further support the use of RSM for media optimization. To the best of our knowledge, this is the first report of statistical media optimization for antibiotic production in rare actinomycete Nonomuraea species, which will be useful for the development of Nonomuraea cultivation process for efficient antibiotic production on a large scale.

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Enhancing production of a 24-membered ring macrolide compound by a marine bacterium using response surface methodology

Cited by 10 publications

References 14 publications

Statistical optimization of lignocellulosic waste containing culture medium for enhanced production of cellulase by Bacillus tequilensis G9

Statistical optimization of lignocellulosic waste containing culture medium for enhanced production of cellulase by Bacillus tequilensis G9

Optimization of cellulase production by Enhydrobacter sp. ACCA2 and its application in biomass saccharification

Successive Nonstatistical and Statistical Approaches for the Improved Antibiotic Activity of Rare ActinomyceteNonomuraeasp. JAJ18

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