Optimization of cellulase production by Enhydrobacter sp. ACCA2 and its application in biomass saccharification

Abstract

Cellulase finds use in saccharification of lignocellulosic agroresidues to fermentable sugars which can be used for production of commercially important metabolites. This study reports endoglucanase (CMCase) production by Enhydrobacter sp. ACCA2. The CMCase activity of the strain ACCA2 was successively improved by optimization of range of physical and nutritional parameter in a set of non-statistical and statistical experiments. Initial non-statistical selection of carbon source, incubation time, temperature and pH resulted in 1.07 fold increase of CMCase activity. In a subsequent statistical method, response surface methodology, optimization of medium components such as carboxymethylcellulose, peptone, NaCl, MgSO4, K2HPO4, and (NH4)2SO4 yielded further increase up to 2.39 fold CMCase activity. The cellulolytic potential was evaluated in biomass saccharification with different plant materials and the results revealed that the enzyme produced by strain may have significant commercial values for industrial saccharification process. Moreover, this is the first report of cellulase production by an Enhydrobacter spp.

Influence of sampling technique and bedding type on the milk microbiota: Results of a pilot study

“…Our study was conducted in Marshfield, Wisconsin, in February 2017, which had temperatures ranging from −19 to 15°C (University of Wisconsin-Madison, 2017), similar to conditions in which Janthinobacterium has been identified (Shivaji et al, 1991). Other environmental bacteria we identified included the genus Enhydrobacter, which has been reported to vary in cellulose-degrading capabilities and has been found in plant composts (Premalatha et al, 2015). Bacteria in this genus have been cultured in microaerophilic conditions (Staley et al, 1987), which differ from the aerobic conditions normally found in bedding microbiology (Hogan et al, 2007).…”

Statistical optimization of lignocellulosic waste containing culture medium for enhanced production of cellulase by Bacillus tequilensis G9

“…The results obtained in this study supported the conclusion of other researchers by further increment (2.07-fold) in activity with RSM design [32,42,43] suggesting that concentration of major medium components is the principal governing factor for cellulase production. However, the sturdy crystalline structure of the cellulose molecules and hemicelluloses of GS limit the accessibility of plant biomass to cellulolytic enzymes [44]. The degradation effect exerted by B. tequilensis G9 on the plant biomass depicted its intricate enzyme activity with lignocellulolytic effect and saccharification potential.…”

Peer Review #1 of "Systematic evaluation of the gut microbiome of swamp eel (Monopterus albus) by 16S rRNA gene sequencing (v0.2)"

“…In the content section, most of the enriched microbial taxa were associated with nutrients metabolism. For example, dietary fiber degradation associated bacteria Enhydrobacter and Comamonadaceae (Premalatha et al, 2015;Sakurai et al, 2017), and amino acid metabolism associated bacteria Caulobacteraceae and Microbacteriaceae (Yin et al, 2017) were enriched in the midgut content. Gut microbial taxa equipped with multiple carbohydrate active enzymes such as Bacteroidaceae, Ruminococcaceae, Coprococcus, and Citrobacter (Luo et al, 2017;Tap et al, 2015;Wu et al, 2012) which involved in non-digestible dietary carbohydrates metabolism showed great abundance in the hindgut content.…”