Optimization of extraction conditions and fatty acid characterization of <i>Lactobacillus pentosus</i> cell‐bound biosurfactant/bioemulsifier

Vecino, Xanel; Barbosa-Pereira, Letricia; Rey, Rosa Devesa; Cruz, J.M.; Moldes, Ana Belén

doi:10.1002/jsfa.6720

Cited by 81 publications

(59 citation statements)

References 26 publications

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“…In fact, we found only one study dedicated to the use of statistical design for the improvement of Bs recovery [48]. They optimized the extraction conditions of a Lactobacillus pentosus cell bound biosurfactant/emulsifier using a Box-Behnken response surface methodology.…”

Section: Improving Downstream Processesmentioning

confidence: 99%

Statistical Design, a Powerful Tool for Optimizing Biosurfactant Production: A Review

Bertrand

Martínez‐Morales

Rosas-Galván

et al. 2018

Colloids and Interfaces

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Biosurfactants (Bs) have been studied for decades and applied in different industrial sectors because of their competitive biochemical characteristics, and the fact that they are environmentally friendly. Current scientific investigations mainly involve the search for novel Bs producing organisms with attractive characteristics. Bs are expected to replace synthetic surfactants in the near future, but low production yields and inefficient downstream processes have prevented their widespread use. Although there are numerous reports on Bs optimization, to date there has been no critical compilation or revision of the statistical designs and strategies employed for improved production. The purpose of this mini review is to briefly discuss the factors that affect Bs production and the importance of statistical design as an essential tool for increasing production.

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Section: Improving Downstream Processesmentioning

confidence: 99%

Statistical Design, a Powerful Tool for Optimizing Biosurfactant Production: A Review

Bertrand

Martínez‐Morales

Rosas-Galván

et al. 2018

Colloids and Interfaces

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“…Moreover, C16:0 and C18:0 were the most abundant fatty acids in the biosurfactant produced by Lactococcus sp. (Saravanakumari and Mani, 2010; Vecino et al, 2014). Many earlier reports demonstrated that the biochemical composition of biosurfactant produced by Bacillus genus varied according to the species.…”

Section: Discussionmentioning

confidence: 99%

Biochemical, Molecular, and Transcriptional Highlights of the Biosynthesis of an Effective Biosurfactant Produced by Bacillus safensis PHA3, a Petroleum-Dwelling Bacteria

2017

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Petroleum crude oil (PCO)-dwelling microorganisms have exceptional biological capabilities to tolerate the toxicity of petroleum contaminants and are therefore promising emulsifier and/or degraders of PCO. This study describes a set of PCO-inhabiting bacterial species, one of which, identified as Bacillus safensis PHA3, produces an efficient biosurfactant which was characterized as a glycolipid. Fourier transform infrared spectrometer, nuclear magnetic resonance, Thin layer chromatography, HPLC, and GC-MS analysis of the purified biosurfactant revealed that the extracted molecule under investigation is likely a mannolipid molecule with a hydrophilic part as mannose and a hydrophobic part as hexadecanoic acid (C16:0). The data reveal that: (i) PHA3 is a potential producer of biosurfactant (9.8 ± 0.5 mg mL-1); (ii) pre-adding 0.15% of the purified glycolipid enhanced the degradation of PCO by approximately 2.5-fold; (iii) the highest emulsifying activity of biosurfactant was found against the PCO and the lowest was against the naphthalene; (iv) the optimal PCO-emulsifying activity was found at 30–60°C, pH 8 and a high salinity. An orthologous gene encodes a putative β-diglucosyldiacylglycerol synthase (β-DGS) was identified in PHA3 and its transcripts were significantly up-regulated by exogenous PAHs, i.e., pyrene and benzo(e)pyrene but much less by mid-chain n-alkanes (ALKs) and fatty acids. Subsequently, the accumulation of β-DGS transcripts coincided with an optimal growth of bacteria and a maximal accumulation of the biosurfactant. Of particular interest, we found that PHA3 actively catalyzed the degradation of PAHs notably the pyrene and benzo(e)pyrene but was much less effective in the mono-terminal oxidation of ALKs. Such characteristics make Bacillus safensis PHA3 a promising model for enhanced microbial oil recovery and environmental remediation.

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“…Advances in biotechnological research have led to the discovery of novel active ingredients, consolidating the development of a new class of products known as biocosmetics . Biosurfactants can play a role in these products, as their surface properties and biological activities make these natural compounds adequate for use in cosmetic applications . Glycolipids (sophorolipids, rhamnolipids, and MELs) and lipopetides (surfactin) are the most widely used biosurfactants in cosmetics due to their multifunctional profile …”

Section: Biosurfactants In the Cosmetic Industrymentioning

confidence: 99%

“…39 Biosurfactants can play a role in these products, as their surface properties and biological activities make these natural compounds adequate for use in cosmetic applications. 40 Glycolipids (sophorolipids, rhamnolipids, and MELs) and lipopetides (surfactin) are the most widely used biosurfactants in cosmetics due to their multifunctional profile. 11 Microemulsions formed by biosurfactants can be used to provide functional components and antioxidants to cosmetics.…”

Section: Natural Products In the Cosmetic Industrymentioning

confidence: 99%

“…The authors state that saponins from C. oleifera have excellent foaming properties and moderate detergency, enabling their use in detergents and cosmetics. 94 The detergent properties of saponins from the mesocarp of the fruit of Balanites aegyptiaca at concentrations (10,20,30,40, and 50% [p/v]) for sebum removal were characterized and compared to sodium dodecyl sulfate, Tween-80 and Triton X-100. The surface tension of the 10, 20, and 30% solutions was similar to that achieved with Triton X-100 (10% p/v) and the detergency of the vegetal material was very high, as the 20% solution led to an 88% sebum removal rate.…”

Section: Dias 77mentioning

confidence: 99%

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Saponins and microbial biosurfactants: Potential raw materials for the formulation of cosmetics

Bezerra

Rufino

Luna

et al. 2018

Biotechnology Progress

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The cosmetic industry is currently one of the fasting growing sections of the economy in many countries. The recent tendency toward the use of cosmetics of a natural origin has driven the industry to seek alternatives to synthetic components in the formulation of products. Biosurfactants are natural compounds that have considerable potential for application in the formulation of safe, effective cosmetics as a replacement for commonly used chemical tensioactive agents. The present review provides essential information on the physicochemical and biological properties of saponins and microbial biosurfactants employed in cosmetic products, with a focus on the use of these natural compounds in shampoos, addressing the current state of research and patents involving biosurfactants for this purpose. The challenges and prospects of this cosmetic application are also discussed. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 2018.

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Optimization of extraction conditions and fatty acid characterization of Lactobacillus pentosus cell‐bound biosurfactant/bioemulsifier

Abstract: The optimum extraction conditions of biosurfactant were achieved at 45 °C at 120 min and using 9 g kg(-1) of salt. In all the assays biosurfactant from L. pentosus yielded more stable emulsions and higher emulsion volumes than polysorbate 20.

Cited by 81 publications

References 26 publications

Statistical Design, a Powerful Tool for Optimizing Biosurfactant Production: A Review

Statistical Design, a Powerful Tool for Optimizing Biosurfactant Production: A Review

Biochemical, Molecular, and Transcriptional Highlights of the Biosynthesis of an Effective Biosurfactant Produced by Bacillus safensis PHA3, a Petroleum-Dwelling Bacteria

Saponins and microbial biosurfactants: Potential raw materials for the formulation of cosmetics

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