Statistical Design, a Powerful Tool for Optimizing Biosurfactant Production: A Review

Bertrand, Brandt; Martínez‐Morales, Fernando; Rosas-Galván, Nashbly Sarela; Morales-Guzmán, Daniel; Trejo‐Hernández, María R.

doi:10.3390/colloids2030036

Cited by 50 publications

(37 citation statements)

References 50 publications

(85 reference statements)

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“…The most widely used statistical designs for the optimisation of media suitable for SAMs (e.g. biosurfactant) production are factorial design and Response Surface Methodologies (RSM) [ 257 ]. In fact, factorial design such as Plackett–Burman Design (PBD) has been employed to optimise the cost-effective culture medium for EPS production in L. rhamnosus by modifying single- and multi-factor-at-a-time (i.e.…”

Section: Challenges Of Lactobacillus Sams Applicatmentioning

confidence: 99%

Vaginal microbiota and the potential of Lactobacillus derivatives in maintaining vaginal health

2020

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Human vagina is colonised by a diverse array of microorganisms that make up the normal microbiota and mycobiota. Lactobacillus is the most frequently isolated microorganism from the healthy human vagina, this includes Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus iners, and Lactobacillus jensenii. These vaginal lactobacilli have been touted to prevent invasion of pathogens by keeping their population in check. However, the disruption of vaginal ecosystem contributes to the overgrowth of pathogens which causes complicated vaginal infections such as bacterial vaginosis (BV), sexually transmitted infections (STIs), and vulvovaginal candidiasis (VVC). Predisposing factors such as menses, pregnancy, sexual practice, uncontrolled usage of antibiotics, and vaginal douching can alter the microbial community. Therefore, the composition of vaginal microbiota serves an important role in determining vagina health. Owing to their Generally Recognised as Safe (GRAS) status, lactobacilli have been widely utilised as one of the alternatives besides conventional antimicrobial treatment against vaginal pathogens for the prevention of chronic vaginitis and the restoration of vaginal ecosystem. In addition, the effectiveness of Lactobacillus as prophylaxis has also been well-founded in long-term administration. This review aimed to highlight the beneficial effects of lactobacilli derivatives (i.e. surface-active molecules) with anti-biofilm, antioxidant, pathogen-inhibition, and immunomodulation activities in developing remedies for vaginal infections. We also discuss the current challenges in the implementation of the use of lactobacilli derivatives in promotion of human health. In the current review, we intend to provide insights for the development of lactobacilli derivatives as a complementary or alternative medicine to conventional probiotic therapy in vaginal health.

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Section: Challenges Of Lactobacillus Sams Applicatmentioning

confidence: 99%

Vaginal microbiota and the potential of Lactobacillus derivatives in maintaining vaginal health

2020

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“…The most requested statistical models are the response surface methodology (RSM) and the factorial design. For optimization, researchers generally evaluate different aspects such as crop conditions and nutritional factors, often including carbon and nitrogen concentrations and their proportions [6].…”

Section: Of 15mentioning

confidence: 99%

“…The global biosurfactant/bioemulsifier market is expected to reach $2.2 billion by 2019, based on a growth rate of 3.5% per year. In addition, the global projected production market is estimated at 476,512.2 tons due to increased demand from Asia, Africa, and Latin America, which account for 21% of total production [6].…”

Section: Of 15mentioning

confidence: 99%

Eco-Friendly Bioemulsifier Production by Mucor circinelloides UCP0001 Isolated from Mangrove Sediments Using Renewable Substrates for Environmental Applications

et al. 2020

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The successful production of a biosurfactant is dependent on the development of processes using low cost raw materials. In the present work, an economically attractive medium composed of corn steep liquor and waste cooking oil was formulated to maximize the production of bioemulsifier by Mucor circinelloides UCP0001. A central rotational composite design was applied to statistical validation of the production. The emulsifying properties, stability under extreme conditions, its toxicity character, and the characterization of the bioemulsifier were determined. The best condition for biomolecule synthesis occurred in the assay 2 containing 4% of corn steep liquor and 3% waste soybean oil and exhibited 100% emulsification index for canola oil and petroleum, as well as excellent emulsifying activity for canola oil and burned engine oil. The nutritional factors studied showed statistical relevance, since all linear, quadratic effects and their interactions were significant. The bioemulsifier showed 2.69 g/L yield and the chemical character of the molecule structure was identified by FT-IR (Fourier Transform Infrared) spectroscopy. The bioemulsifier showed no toxicity to Artemia salina and Chlorella vulgaris. Stable emulsions were obtained under extreme conditions of temperature, pH, and salinity. These findings contribute to understanding of the relationship between production, physical properties, chemical composition, and stability of bioemulsifier for their potential applications in biotechnology, such as bioremediation of hydrocarbon-contaminated soil and water.

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“…A few decades ago, the traditional one-factor-at-a-time (OFAT) technique dominated literature and still applies today in the early stages of bioprocess optimization (Nicolo et al 2017 ). This method, with its inherent limitations of tedium and inability to account for interaction effects between and among independent variables, has been largely replaced with more robust techniques like response surface methodology (RSM) and artificial neural network (ANN) (Bertrand et al 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Statistical and Artificial Neural Network Approaches to Modeling and Optimization of Fermentation Conditions for Production of a Surface/Bioactive Glyco-lipo-peptide

Ekpenyong

Asitok

Antai

et al. 2020

Int J Pept Res Ther

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A freshwater alkaliphilic strain of Pseudomonas aeruginosa, grown on waste frying oil-basal medium, produced a surface-active metabolite identified as glycolipopeptide. Bioprocess conditions namely temperature, pH, agitation and duration were comparatively modeled using statistical and artificial neural network (ANN) methods to predict and optimize product yield using the matrix of a central composite rotatable design (CCRD). Response surface methodology (RSM) was the statistical approach while a feed-forward neural network, trained with Levenberg–Marquardt back-propagation algorithm, was the neural network method. Glycolipopeptide model was predicted by a significant ( P < 0.001, R 2 of 0.9923) quadratic function of the RSM with a mean squared error (MSE) of 3.6661. The neural network model, on the other hand, returned an R 2 value of 0.9964 with an MSE of 1.7844. From all error metrics considered, ANN glycolipopeptide model significantly ( P < 0.01) outperformed RSM counterpart in predictive modeling capability. Optimization of factor levels for maximum glycolipopeptide concentration produced bioprocess conditions of 32 °C for temperature, 7.6 for pH, agitation speed of 130 rpm and a fermentation time of 66 h, at a combined desirability function of 0.872. The glycosylated lipid-tailed peptide demonstrated significant anti-bacterial activity (MIC = 8.125 µg/mL) against Proteus vulgaris , dose-dependent anti-biofilm activities against Escherichia coli (83%) and Candida dubliniensis (90%) in 24 h and an equally dose-dependent cytotoxic activity against human breast (MCF-7: IC50 = 65.12 µg/mL) and cervical (HeLa: IC50 = 16.44 µg/mL) cancer cell lines. The glycolipopeptide compound is recommended for further studies and trials for application in human cancer therapy.

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Statistical Design, a Powerful Tool for Optimizing Biosurfactant Production: A Review

Cited by 50 publications

References 50 publications

Vaginal microbiota and the potential of Lactobacillus derivatives in maintaining vaginal health

Vaginal microbiota and the potential of Lactobacillus derivatives in maintaining vaginal health

Eco-Friendly Bioemulsifier Production by Mucor circinelloides UCP0001 Isolated from Mangrove Sediments Using Renewable Substrates for Environmental Applications

Statistical and Artificial Neural Network Approaches to Modeling and Optimization of Fermentation Conditions for Production of a Surface/Bioactive Glyco-lipo-peptide

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