Optimization and comparison of different modes of preparative chromatography

Abstract

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Evaluation of cellulose-based biospecific adsorbents as a stationary phase for lectin affinity chromatography

“…The prediction of the behavior of affinity systems during the washing and desorption stage requires the knowledge of the effective dissociation constant (K d ) which reflects the strength of the ligand-sorbate binding. Thus, the kinetic data is the basis for the evaluation of a new affinity medium [7] as well as for the modelling and optimization of the sorptive bioseparation processes [8,9].…”

Trends in Preparative HPLC

“…A systematic investigation (theoretical simulation on the basis of determined adsorption isotherms and experimental verification) to compare the different chromatographic modes has recently been published by Seidel-Morgenstern for the separation of a binary mixture consisting of two isomers of a steroid [41,42]. The concentrations of component i in the liquid and in the solid phases, C i and q i , respectively, are related through the adsorption isotherms [equation ].…”

“…Several methods (e.g., frontal analysis, elution by characteristic point, minor disturbance method, adsorption-desorption, and chromatogram fitting) are available for the determination of the equilibrium data and have been reviewed by Nicoud and Seidel-Morgenstern [43] and very recently by Seidel-Morgenstern [44]. It is beyond the scope of this chapter to describe all methods with their benefits and drawbacks in detail, and the interested reader is referred to the literature [i.e., [39][40][41][42][43][44]. Nevertheless, three methods (given below) that we have used in our laboratories are briefly summarized to illustrated the underlying principles.…”

Chromatographic Models as Tools for Scale‐up of Isolation of Natural Products by Semi‐preparative HPLC

“…[16] Recently, computer programmes have been used for the economic and operational optimization of HPLC at large scale. [8][9][10][11] Experimental cases of scale-up for peptides and proteins-based samples using models have been published. [12][13][14] However, for low molecular weight compounds, computerassisted prediction has only been used for the separation of binary aromatic chemicals and chiral isomers ( Table 1).…”

“…Direct scale-up of semi-preparative separation of natural compounds, 180 Mazzei and d'Avila ©2002 Marcel Dekker, Inc. All rights reserved. This material may not be used or reproduced in any form without the express written permission of Marcel Dekker, Inc. Non-ideal rate [11] a Also using direct scale-up.…”