Trends in Preparative HPLC

Kuesters, Ernst

doi:10.1002/9780470087954.ch21

Cited by 1 publication

(1 citation statement)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As the decisive thermodynamic functions have to be determined experimentally, the boundary conditions of the chromatographic separation are important factors. The two methods most suitable to this separation, the elution by characteristic point (ECP) [34] and the minor disturbance method (MDM) [35], were selected. The component solubilities, the solvent composition and flow rate of the mobile phase are related to the maximum feed concentrations for a given stationary phase [36] and determine the efficiency of a simulated moving bed process.…”

Section: Introductionmentioning

confidence: 99%

Preparative-scale separation by simulated moving bed chromatography of biocatalytically produced regioisomeric lactones

Kaiser¹,

Ottolina

Carrea

et al. 2009

New Biotechnology

View full text Add to dashboard Cite

The separation of enantiomerically pure, but regioisomeric compounds by chromatography is attractive for the production of chiral compounds, if the process leads to high-purity products in high yield with excellent throughput and reduced solvent use. The biocatalytic Baeyer-Villiger oxidation of racemic ketones gives rapid access to an equimolar mixture of regioisomeric lactones with excellent enantiomeric purity. The existing separation methods have so far represented a bottleneck for preparative applications of this technology. Simulated moving bed chromatography is described in this work as an efficient and scalable separation technology able to meet the goals of both high efficiency as well as reduced solvent use. The main factors influencing method optimisation were identified and the parameters of temperature, pressure, weak adsorption, separation factor and robustness were examined with the aim to resolve bottlenecks. Under optimised conditions, the productivity of the process was 0.026g/(g(stationary)(phase) day) of pure regioisomers and the solvent consumption was 0.363L/g(feed).

show abstract

Section: Introductionmentioning

confidence: 99%