Chromatographic Models as Tools for Scale‐up of Isolation of Natural Products by Semi‐preparative HPLC

Mazzei, José Luiz; d’Avila, Luiz Antonio

doi:10.1081/jlc-120017162

Cited by 21 publications

(11 citation statements)

References 19 publications

(17 reference statements)

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“…Both column and thin layer chromatographic techniques are being used for the purification of carotenoids (Oliver and Palou 2000;Ladislav et al 2005). Preparative HPLC techniques permit rapid, reliable and reproducible isolation and purification of carotenoids (Tan 1988;Scalia and Francis 1989;Mazzei and d'Avila 2003). High speed counter current chromatography has also been demonstrated as a effective tool for the preparation and purification of carotenoids from plant sources (Aman et al 2005;Chen et al 2005;Tsao and Yang 2006).…”

Section: Separation Detection and Quantificationmentioning

confidence: 99%

Red pepper (Capsicum annuum) carotenoids as a source of natural food colors: analysis and stability—a review

et al. 2014

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Carotenoids are increasingly drawing the attention of researchers as a major natural food color due to their inherent nutritional characteristics and the implicated possible role in prevention and protection against degenerative diseases. In this report, we review the role of red pepper as a source for natural carotenoids. The composition of the carotenoids in red pepper and the application of different methodologies for their analysis were discussed in this report. The stability of red pepper carotenoids during post-harvest processing and storage is also reviewed. This review highlights the potential of red pepper carotenoids as a source of natural food colors and also discusses the need for a standardized approach for the analysis and reporting of composition of carotenoids in plant products and designing model systems for stability studies.

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Section: Separation Detection and Quantificationmentioning

confidence: 99%

Red pepper (Capsicum annuum) carotenoids as a source of natural food colors: analysis and stability—a review

et al. 2014

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“…The theory value of flow rate for prep‐HPLC was calculated according to the following formula based on analytical HPLC conditions .

F_{normalp} = F_{normala} \times {(D_{normalp} / D_{normala})}^{2}

where F p is the flow rate in prep‐HPLC; F a is the flow rate in analytical HPLC; D p is the diameter of prep‐HPLC column; and D a is the diameter of analytical HPLC column.…”

Section: Resultsmentioning

confidence: 99%

Separation of three anthraquinone glycosides including two isomers by preparative high‐performance liquid chromatography and high‐speed countercurrent chromatography fromRheum tanguticumMaxim. ex Balf

Chen

Zou

et al. 2016

J of Separation Science

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Anthraquinone glycosides, such as chrysophanol 1-O-β-d-glucoside, chrysophanol 8-O-β-d-glucoside, and physion 8-O-β-d-glucoside, are the accepted important active components of Rheum tanguticum Maxim. ex Balf. due to their pharmacological properties: antifungal, antimicrobial, cytotoxic, and antioxidant activities. However, an effective method for the separation of the above-mentioned anthraquinone glycosides from this herb is not currently available. Especially, greater difficulty existed in the separation of the two isomers chrysophanol 1-O-β-d-glucoside and chrysophanol 8-O-β-d-glucoside. This study demonstrated an efficient strategy based on preparative high-performance liquid chromatography and high-speed countercurrent chromatography for the separation of the above-mentioned anthraquinone glycosides from Rheum tanguticum Maxim.ex Balf.

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“…To solve this problem, semipreparative HPLC technique was used. Semipreparative HPLC can provide an on-line chromatogram for each peak in the herb extract [22,23]. In most cases, the purity of the isolated compound and the conveniences of the procedure are satisfactory [24,25].…”

Section: Purification Of Z-ligustilide Using Semipreparative Hplcmentioning

confidence: 99%

A new procedure for the preparative separation and isolation of Z‐ligustilide from the roots of Angelica sinensis

Zhang²,

Xie

et al. 2007

J of Separation Science

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A new procedure for the separation and isolation of Z-ligustilide from the roots of Angelica sinensis (AS) was developed, and the storage conditions for Z-ligustilide were optimized. Using the present procedure, Z-ligustilide was enriched by decomposing the Z-ligustilide dimers yielding Z-ligustilide and dissolving the polar impurities in hot water. Then, the crude Z-ligustilide was further purified by a semipreparative HPLC system. The spiked and nonspiked samples were used for the evaluation of the proposed procedure. Recoveries obtained varied from 86.2 to 90.7% and RSDs from 4.0 to 6.6%. The yield and purity of the isolated Z-ligustilide were found to be 4.57 mg/g and 99.6%, respectively. The results of stability tests have shown that the presence of oxidant contributes to Z-ligustilide degradation, therefore argon was chosen as a shielding gas for storage. The overall procedure is efficient and convenient which is considered suitable for the preparative separation of Z-ligustilide from AS.

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Chromatographic Models as Tools for Scale‐up of Isolation of Natural Products by Semi‐preparative HPLC

Cited by 21 publications

References 19 publications

Red pepper (Capsicum annuum) carotenoids as a source of natural food colors: analysis and stability—a review

Red pepper (Capsicum annuum) carotenoids as a source of natural food colors: analysis and stability—a review

Separation of three anthraquinone glycosides including two isomers by preparative high‐performance liquid chromatography and high‐speed countercurrent chromatography fromRheum tanguticumMaxim. ex Balf

A new procedure for the preparative separation and isolation of Z‐ligustilide from the roots of Angelica sinensis

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